International Journal of Fisheries and Aquaculture
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Article Number - C72434655695


Vol.10(1), pp. 1-7 , January 2018
https://doi.org/10.5897/IJFA2016.0611
ISSN: 2006-9839


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Full Length Research Paper

Effects of Lead Nitrate on catalase production levels in post juvenile Clarias gariepinus (Burchell, 1822)



Okoye C. Loveline
  • Okoye C. Loveline
  • Hydrobiology and Fisheries Unit, Department of Biological Sciences, Federal University of Technology, Minna, Niger State, Nigeria.
  • Google Scholar
Patrick Ozovehe Samuel
  • Patrick Ozovehe Samuel
  • Hydrobiology and Fisheries Unit, Department of Biological Sciences, Federal University of Technology, Minna, Niger State, Nigeria.
  • Google Scholar
Arimoro, F. O.,
  • Arimoro, F. O.,
  • Hydrobiology and Fisheries Unit, Department of Biological Sciences, Federal University of Technology, Minna, Niger State, Nigeria.
  • Google Scholar
Ayanwale, A. V.
  • Ayanwale, A. V.
  • Hydrobiology and Fisheries Unit, Department of Biological Sciences, Federal University of Technology, Minna, Niger State, Nigeria.
  • Google Scholar
Auta, Y. I.
  • Auta, Y. I.
  • Hydrobiology and Fisheries Unit, Department of Biological Sciences, Federal University of Technology, Minna, Niger State, Nigeria.
  • Google Scholar
Muhammed, A. Z.
  • Muhammed, A. Z.
  • Hydrobiology and Fisheries Unit, Department of Biological Sciences, Federal University of Technology, Minna, Niger State, Nigeria.
  • Google Scholar







 Received: 19 December 2016  Accepted: 04 April 2017  Published: 31 January 2018

Copyright © 2018 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The effects of Lead Nitrate on catalase (CAT) production levels in post juvenile Clarias gariepinus were investigated. A total of 250 samples of the fish were acclimatized for two week. Six samples of the fish were exposed to the sub-lethal concentrations of 00, 28, 43 and 57 mg/L with replicate in each case for 28 days. Two samples of the fish were randomly selected from the treatments and control for the bioassay of catalase on the 14th and 28th day respectively. The fish organs (kidney, liver and gill) were excised from the fish and homogenized in sodium phosphate buffer (0.2 M, pH 8.0). The data generated were subjected to one-way analysis of variance followed by Duncan Multiple Range test where significant. The results showed that 96 h LC50 of C. gariepinus exposed to lethal concentrations of Lead Nitrate was 284.189 mg/L. The CAT production levels were significantly higher in the kidney of the fish exposed in 28 mg/L on day 14; 43 and 57 mg/L on day 28, respectively. The CAT production levels were significantly higher in the gill of the fish exposed in 57 mg/L on day 14; 43 and 57 mg/L on day 28 respectively. The CAT production levels were significantly higher in the liver of the fish exposed in 28 and 57 mg/L on day 14 respectively. The highest production mean values of 149.55±43.65 and 152.80±40.40 U/mgprotein were obtained in the kidney of the fish exposed in 57 and 43 mg/L, respectively. Therefore, the kidney of the fish exhibited a better control of the toxicant and as such, catalase production level in this organ should be used in assessing the level of physiological changes in the fish.

 

Key words: Catalase, Lead Nitrate, oxidative stress, Clarias gariepinus, LC50.

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APA Okoye, C. L., Patrick, O. S., Arimoro, F. O., Ayanwale, A. V., Auta, Y. I., & Muhammed, A. Z. (2018). Effects of Lead Nitrate on catalase production levels in post juvenile Clarias gariepinus (Burchell, 1822). International Journal of Fisheries and Aquaculture, 10(1), 1-7.
Chicago Okoye C. Loveline, Patrick Ozovehe Samuel, Arimoro, F. O., Ayanwale, A. V., Auta, Y. I. and Muhammed, A. Z.. "Effects of Lead Nitrate on catalase production levels in post juvenile Clarias gariepinus (Burchell, 1822)." International Journal of Fisheries and Aquaculture 10, no. 1 (2018): 1-7.
MLA Okoye C. Loveline, et al. "Effects of Lead Nitrate on catalase production levels in post juvenile Clarias gariepinus (Burchell, 1822)." International Journal of Fisheries and Aquaculture 10.1 (2018): 1-7.
   
DOI https://doi.org/10.5897/IJFA2016.0611
URL http://academicjournals.org/journal/IJFA/article-abstract/C72434655695

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