International Journal of Genetics and Molecular Biology
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Article Number - A2E80622565


Vol.4(1), pp. 10-15 , November 2012
DOI: 10.5897/IJGMB12.003
ISSN: 2006-9863



Full Length Research Paper

Evaluation of genetic heterogeneity in glutamate carboxypeptidase II (H475Y) and reduced folate carrier (SLC19A1) gene variants increased risk factor for the development of neural tube defects in eastern region of India


Ajit Kumar Saxena1*, S. Pandey1 and L. K. Pandey2




1Human Molecular Cytogenetic Laboratory, Centre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, India.

2Department of Obstetrics and Gynecology, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, India


Email: draksaxena1@rediffmail.com






 Accepted: 03 May 2012  Published: 30 November 2012

Copyright © 2012 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


In humans, neural tube failure to close during the 4th week of gestation leads to the development of severe congenital malformations of the central nervous system because of an error in maternal folate metabolism associated gene variants. The frequency of genotypic variants of GCP II (H475Y) and folate carrier RFCI (SLC19A1) gene polymorphism (80 G →A) were evaluated as potential candidate gene(s) and also assess their clinical association to increase “risk” in neural tube defects (NTDs). In the present study, blood samples (0.5 ml) were collected from NTD cases, mother and their respective controls and genomic DNA was isolated to evaluate the impact of GCP II and RFCI genotypic variants as risk factor using polymerase chain reaction (PCR) based restriction fragment length polymorphism (RFLP) analysis. Significant differences (p<0.05) were observed between case mothers and control for GCP II genotype using Fischer’s exact two tailed probability test. The odd ratio was calculated to determine the risk factors at 95% C.I. (1.56-87.60), which seems to be very high, suggesting significant involvement of GCP II gene in the development of NTDs. The significant (p = 0.03) risk factor was also calculated (OR=4.85: 95%, C.I. 1.33-17.36) forRFCI gene between heterozygote (GA) and homozygote (AA) mothers having NTDs child. The present finding strongly suggests that genotype variants of GCPII and RFCI gene, in heterozygous condition, are responsible for increasing as independent risk factor for the development of NTDs like meningomyelocele (MMC) susceptibility in this region.

 

Key words: Glutamate carboxy peptidase, reduced folate carrier, gene polymorphism, neural tube defects, meningomyelocele.

 

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APA (2012). Evaluation of genetic heterogeneity in glutamate carboxypeptidase II (H475Y) and reduced folate carrier (SLC19A1) gene variants increased risk factor for the development of neural tube defects in eastern region of India. International Journal of Genetics and Molecular Biology, 4(1), 10-15.
Chicago Ajit Kumar Saxena, S. Pandey and L. K. Pandey. "Evaluation of genetic heterogeneity in glutamate carboxypeptidase II (H475Y) and reduced folate carrier (SLC19A1) gene variants increased risk factor for the development of neural tube defects in eastern region of India." International Journal of Genetics and Molecular Biology 4, no. 1 (2012): 10-15.
MLA Ajit Kumar Saxena, et al. "Evaluation of genetic heterogeneity in glutamate carboxypeptidase II (H475Y) and reduced folate carrier (SLC19A1) gene variants increased risk factor for the development of neural tube defects in eastern region of India." International Journal of Genetics and Molecular Biology 4.1 (2012): 10-15.
   
DOI 10.5897/IJGMB12.003
URL http://academicjournals.org/journal/IJGMB/article-abstract/A2E80622565

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