International Journal of Genetics and Molecular Biology
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Article Number - C2F08DC58961


Vol.8(2), pp. 7-10 , May 2016
DOI: 10.5897/IJGMB2013.0093
ISSN: 2006-9863



Full Length Research Paper

Genotypic restriction patterns of finger millet (Eleusine coracana (l.) Gaertn) landraces in northern Nigeria



Umar I. D.
  • Umar I. D.
  • Department of Biological Sciences, University of Abuja, Abuja Nigeria.
  • Google Scholar
Kwon-Ndung E. H.
  • Kwon-Ndung E. H.
  • Department of Biological Sciences, Federal University, Lafia, Nasarawa State, Nigeria.
  • Google Scholar
Mailafia S.
  • Mailafia S.
  • Department of Veterinary Microbiology, University of Abuja, Abuja Nigeria.
  • Google Scholar







 Received: 09 December 2013  Accepted: 01 July 2015  Published: 31 May 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The present study was conducted to characterize the genetic diversity of 10 germplasm accessions of finger millet (Eleusine coracana (L) Gaertn) collected from diverse locations spread across the geographical zone of Northern Nigeria.  The digest was carried out using the molecular marker Restriction Fragment Digest (RFD on agarose gel while EcoR1 and Hind III restriction enzymes were used to cut the genomic DNA at specific sites. The results of our findings using RFD generated four clear DNA bands of molecular weights ranging from 10000, 8500, 1000 and 200 KDA. This clearly suggests the existence of polymorphism among the plant accessions.  These results demonstrate the high variability that exists amongst the genetic traits for these germplasm accessions. Information on this plant is very useful in unraveling the pedigree genetic relationships, as well as, in designing breeding and selection experiments for improvement of this crop. It will further stimulate growing interest on the genetic diversity and classification of this crop.

 

Key words: Genotypic, restriction digestion, finger millet, Eco R1, Hind 111, northern Nigeria.

Das, B., Jena, R. and Samal, K. (2008). Optimisation of DNA Isolation and PCR Protocol for RAPD analyses of Banana/Plantain (Musa spp). Int. J. Agric Sci. 1:21-25.

 

Gupta R, Krishan V, Dinesh Y, Munna S (2010). Assessment of Genetic Relatedness among Three Varieties of Finger Millet with Variable Seed Coat Color Using RAPD and ISSR Markers. Genet. Eng. Biotechnol. J. pp. 1-9.

 
 

Hibbett DS, Nilson RH, Snyder M, Fonseca M, Constanz C, Shonfeld M (2005). Automated Phylogenetic taxonomy: An example in Homobasidiomycetes (mushroom-forming fungi). Syst. Biol. 54(4):660-668.
Crossref

 
 

Hoefer Inc. (2008). DNA electrophoresis. 

 
 

Kanchan K, Pande A (2010). Study of genetic diversity in finger millet (Eleusine coracana L. Gaertn) using RAPD markers. Afr. J. Biotechnol. 9(29):4542-4549.

 
 

Murray S, Sharma A, Rooney W, Klein P (2008). Genetic improvement of sorghum as a biofuel feedstock. Crop Sci. 48:2165-2179.
Crossref

 
 

Padmalatha K, Prasad MNV (2006). Optimisation of DNA Isolation and PCR protocol for RAPD Analysis of selected medicinal and aromatic plants of conservation concern from Peninsular, Indian. Afr. J. Biotechnol. 5(3):230-234.

 
 

Rocha LS, Falqueto CBS, Grimaldi GJ, Polillo E (2006). Genetic structure of Lutzomia (Nyssomyia) intermedia populations from two ecologic regions in Brazil where transmission of Leishmania reflects distinct eco-epidemiologic features. Am. J. Trop. Med. Hyg. 76(3):559-565.

 
 

Sakallah AS, Lanning RW, Cooper DL (1995). DNA Fingerprinting of Crude Bacterial Lysates using degenerate RAPD primers. Genome Res. 4:265-268.
Crossref

 
 

Salimath SS, De Oliveira AC, Godwin ID, Bennetzen JL (1995). Assessment of genome origins and genetic diversity in the genus Eleusine with DNA markers. Genome 38:757-763.
Crossref

 

 


APA Umar, I. D., Kwon-Ndung, E. H., & Mailafia, S. (2016). Genotypic restriction patterns of finger millet (Eleusine coracana (l.) Gaertn) landraces in northern Nigeria. International Journal of Genetics and Molecular Biology, 8(2), 7-10.
Chicago Umar I. D., Kwon-Ndung E. H. and Mailafia S.. "Genotypic restriction patterns of finger millet (Eleusine coracana (l.) Gaertn) landraces in northern Nigeria." International Journal of Genetics and Molecular Biology 8, no. 2 (2016): 7-10.
MLA Umar I. D., Kwon-Ndung E. H. and Mailafia S.. "Genotypic restriction patterns of finger millet (Eleusine coracana (l.) Gaertn) landraces in northern Nigeria." International Journal of Genetics and Molecular Biology 8.2 (2016): 7-10.
   
DOI 10.5897/IJGMB2013.0093
URL http://academicjournals.org/journal/IJGMB/article-abstract/C2F08DC58961

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