International Journal of Nutrition and Metabolism
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Article Number - 0F100FD67202


Vol.10(9), pp. 75-81 , December 2017
https://doi.org/10.5897/IJNAM2016.0203
ISSN: 2141-2332


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Full Length Research Paper

Potential probiotics from traditional fermented milk, Mursik of Kenya



Digo C. A.
  • Digo C. A.
  • Department of Human Nutrition, Egerton University, South Africa.
  • Google Scholar
Kamau-Mbuthia E.
  • Kamau-Mbuthia E.
  • Department of Human Nutrition, Egerton University, South Africa.
  • Google Scholar
Matofari J. W.
  • Matofari J. W.
  • Department of Dairy, Food Science and Technology, Egerton University, South Africa.
  • Google Scholar
Ng’etich W. K
  • Ng’etich W. K
  • College of Science, Engineering, and Agriculture, University of Kwa Zulu Natal, South Africa.
  • Google Scholar







 Received: 20 July 2016  Accepted: 27 September 2016  Published: 31 December 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Commercial fermented milks have health benefit potential attributed to pure probiotics used as starter culture. Lactic acid bacteria (LABs) are the probiotics and promote stabilization of the gastrointestinal microecology of humans by producing secondary metabolites like lactic, acetic and propionic acid, hydrogen peroxide and bacteriocins (bactericidal proteins produced by the lactic acid bacteria). These metabolites are health benefiting. Mursik, an indigenous fermented milk product is consumed at households by many Kenyan communities, for example Kalenjin. The fermentation is not based on pure cultures, instead it is spontaneous. Whether the type and concentration of the probiotics in this traditionally fermented milk have the same probiotic potential as those of commercial fermented milk is not documented. This study aimed at isolating mursik probiotics and determining their probiotic potential. Mursik was obtained from informal women groups and individuals involved in small scale production and marketing in Bomet County, Kenya. Forty one (41) samples of mursik were collected. Probiotic isolation was done by pour plate method and the probiotic potential was done by using disc diffusion method against Salmonella enteritica ATCC 13076, Escherichia coli ATCC 25922 and Staphylococcus aureus isolate which were used as standard strains of public health concern. Data was analyzed using analysis of variance (ANOVA) and chi-square tests at α = 0.05. The main probiotics isolated from mursik include Lactobacillus plantarum, Lactobacillus fermentum, Lactobacillus brevis and Lactobacillus casei. The metabolites produced by the isolated probiotics in broth demonstrated significant (P<0.05) antibacterial effect against the standard strains used. The results obtained from this study confirm that the probiotics in mursik, a traditionally fermented milk product have significant potential against enteric and environmental pathogens that are of public health concern.

Key words:  Mursik, probiotic, lactic acid bacteria, antibacterial activity, Lactobacillus.

Ashton QA (2013). Lactobacillus – Adv. in Res. and App. Atlanta, Georgia: Scholarly Editions.

 

Barakat OS, Ibrahim GA, Tawfik NF, El-kholy WI, Gad el-rab DA (2011). Identification and probiotic characteristics of Lactobacillus Strains isolated from traditional domiati cheese. Int. J. Microbiol. Res. 3:59-66.
Crossref

 
 

Bastos MCF, Coelho MLV, Santos OCS (2015). Resistance to bacteriocins produced by Gram-positive bacteria. Microbiology 161:683-700.
Crossref

 
 

Bauer AW, Kirby WM, Sherris JC (1966). Antibiotic susceptibility testing by a single disc method. Am. J. Pathol. 45:493-496.

 
 

Bergey DH, Harrison FC, Breed RS, Hammer BW, Huntoon FM (1989). Bergey's Manual of Determinative Bacteriology (Vol. 9). London: William and Wilkins Company.

 
 

Bezkorvainy A (2001). Probiotics: Determinants of survival and growth in the gut. Am. J. Clin. Nutr. 73:399-405.

 
 

Buntin N, Chanthachum S, Hongpattarakere T (2008). Screening of lactic acid bacteria from gastrointestinaltracts of marine fish for their potential use as pro-biotics. Songklanakarin J. Sci. Technol. 30:141-148.

 
 

Chuayana EL, Ponce C, Rivera RB, Cabrera E (2003). Antimicrobial activity of probiotics from milk products. Phil. J. Microbiol. Infect. Dis. 32(2):71-74.

 
 

Coelho MLV, Coutinho BG, Santos OCS, Nes IF, Bastos MCF (2014). Immunity to the Staphylococcus aureus leaderless four-peptide bacteriocin aureocin A70 is conferred by AurI, an integral membrane protein. Res. Microbiol. 165:50-59.
Crossref

 
 

Dardir HA (2012). In vitro evaluation of probiotic activities of lactic acid bacteria strains isolated from novel probiotic dairy products. Glob. Vet. 8(2):190-196.

 
 

De Vuyst L, Leroy F (2007). Bacteriocins from lactic acid bacteria: production, purification and food application. J. Mol. Microbiol. Biotechnol. 13(4):1-6.
Crossref

 
 

Elaine M, Danilo M (2012). Diversification booklet number 21: Traditional fermented food and beverage for improved livelihoods. Rome: Food and Agriculture Organization (FAO) of the UN.

 
 

Fernandez MF, Boris S, Barbes C (2003). Probiotic properties of human lactobacilli strains to be used in the gastrointestinal tract. J. Appl. Microbiol. 94:449-455.
Crossref

 
 

Hammes WP, Vogel RF (1995). The genus Lactobacillus. The Genera of Lactic Acid Bacteria. UK.: B.J.B. Wood Blackie Academic and Professional.

 
 

Hansen JJ, Sartor RB (2015). Therapeutic manipulation of the microbiome in IBD: current results and future approaches. Curr. Treat. Options Gastroenterol. 13(1):105-20.
Crossref

 
 

Holzapfel WH (2002). Apt starter culture technologies for small-scale fermentation in developing countries. Int. J. Food Microbiol. 75(3):197-212.
Crossref

 
 

Huttenhower C, Kostic AD, Xavier RJ (2014). Inflammatory bowel disease as a model for translating the microbiome. Immuniy 40:843-854.
Crossref

 
 

Jafari B, Rezaie A, Siamak A (2011). Isolation and Identification of potentially probiotic bacteria from Traditional Dairy Products of Ardabil region in Iran. Ann. Biol. Res. 2(6):311-317.

 
 

Klaenhammer TR, Kullen MJ (1999). Selection and design probiotics. Int. J. Food Microbiol. 50(1):45-57.
Crossref

 
 

Maragkoudakis PA, Zoumpopoulou G, Miaris C, Kalantzopoulos G, Pot B, Tsakalidou E (2005). Probiotic potential of Lactobacillus strains isolated from dairy products. Int. Dairy J. 16:189-199.
Crossref

 
 

Mohnl M, Acosta AY, Ojeda AA, Rodriguez SB, Pasteiner S (2007). Effect of synbiotic feed additive in comparison to antibiotic growth promoter on performance and health status of broilers. Poult. Sci. 86(1):217.

 
 

Muigei SC, Shitandi A, Muliro P, Ogata RB (2013). Production of Exopolysaccharides in the Kenyan Fermented Milk, Mursik. Int. J. Sci. Res. ISSN (Online):2319-7064.

 
 

Muinde A (2011). Popular milk, mursik now in shops. Retrieved June 14, 2016, from Farmbiz Africa: 

View

 
 

Ogunbanwo S, Sanni A, Onilude A (2003). Influence of cultural conditions on the production of bacteriocins by Lactobacillus brevis OG1. Afr. J. Biotechnol. 2(7):179-184.
Crossref

 
 

Ouwenhand AC, Tuomola EM, Tolkko S, Salminen S (2001). Assessment of adhesion properties of novel probiotic strains to human intestinal mucus. Int. J. Food Microbiol. 64:119-126.
Crossref

 
 

Quwehand A, Salminen S, Isolauri E (2002). Probiotics: an overview of beneficial effects. Antonie Van Leeuwenhoek 82(1-4):279-289.
Crossref

 
 

Rana AS, Mehta S, Pandhi ND (2011). Isolation and Characterization of Intestinal Bactria as Potential Probiotics. Functional food - A new concept for sustainable livelihood. Junagardh: Dept. of Biotechnol. Agric. Uni. Junagardh. pp. 117-126.

 
 

Roos S, Engstrand L, Jonsson H (2005). Lactobacillus gastricus sp. nov., Lactobacillus antri sp. nov., Lactobacillus kalixensis sp. nov., and Lactobacillus ultinesiss sp. nov. Isolated from human stomach mucosa. Int. J. Syst. Evol. Microbiol. 55:77-78.
Crossref

 
 

Shukla G, Sharma G, Goyal N (2010). Probiotic Characterization of Lactobacilli and Yeast Strains Isolated from Whey Beverage and Therapeutic Potential of Lactobacillus Yoghurt in Murine Giardiasis. Am. J. Biomed. Sci. 2:248.
Crossref

 
 

Srinu B, Madhava RT, Kondal RK, Venkata MR (2012). Evaluation of probiotic characteristics in certain lactic acid bacterial strains by in vitro techniques. Acta Biol. Ind. 1(2):149-154.

 
 

Sullivan LO, Ross RP, Hill C (2002). Potential of bacteriocin-producing lactic acid bacteria for improvements in food safety and quality. Biochemie 84(5-6):593-604.
Crossref

 
 

Tambekar DH, Bhutada SA (2010). An evaluation of probiotic potential of Lactobacillus Sp. from milk of domestic animals and commercial available probiotic preparations in prevention of enteric bacterial infections. Recent Res. Sci. Technol. 2(10):82-88.

 
 

Tanaka H, Doesburg K, Iwasaki T, Mireau I (1999). Screening of lactic acid bacteria for bile salt hydrolase activity. J. Dairy Sci. 82:2530-2535.
Crossref

 
 

Zoumpopoulou G, Foligne B, Christodoulou K, Grangette C, Pot B, Tsakalidou E (2008). Lactobacillus fermentum ACA-AD 179 displays probiotic potential in vitro and protects against trinitrobenzene sulfonic acid (TNBS)-induced colitis and salmonella infection in murine models. Int. J. Food Microbiol. 121:18-26.
Crossref

 

 


APA Digo, C. A., Kamau-Mbuthia, E., Matofari, J. W., & Ng’etich, W. K. (2017). Potential probiotics from traditional fermented milk, Mursik of Kenya. International Journal of Nutrition and Metabolism, 10(9), 75-81.
Chicago Digo C. A., Kamau-Mbuthia E., Matofari J. W. and Ng’etich W. K.. "Potential probiotics from traditional fermented milk, Mursik of Kenya." International Journal of Nutrition and Metabolism 10, no. 9 (2017): 75-81.
MLA Digo C. A., et al. "Potential probiotics from traditional fermented milk, Mursik of Kenya." International Journal of Nutrition and Metabolism 10.9 (2017): 75-81.
   
DOI https://doi.org/10.5897/IJNAM2016.0203
URL http://academicjournals.org/journal/IJNAM/article-abstract/0F100FD67202

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