Triglyceride lowering by chromium picolinate in type 2 diabetic people

In some studies in diabetic people, chromium picolinate supplementation has lowered readings for both plasma triglycerides and blood sugar. In the present study, relatively low dose supplementation (200 μg chromium) did not lower blood sugar readings, but did lower triglyceride values in people with the following characteristics: type 2 diabetes, moderately elevated blood sugar, and not using insulin therapy. These results support the supposition that chromium picolinate can affect triglyceride concentrations independently of effects on carbohydrate metabolism.


INTRODUCTION
Chromium (Cr) is a trace element that can affect carbohydrate, lipid, and protein metabolism (Anderson, 1998a;Evans, 1989). Cr supplementation can affect blood glucose, total cholesterol, HDL and triglycerides in some circumstances (Abdollahi et al., 2013;Anderson et al., 1997;Cefalu and Hu, 2004;Heimbach and Anderson, 2005;Morris et al., 1999). This has led to classifying Cr as an essential nutrient (Anderson, 1998b), though others have suggested that Cr acts only as a drug in some unhealthy situations (Vincent, 2013). One argument against the essential nutrient role has been a lack of consistent positive effects of Cr supplementation in healthy people (Masharani et al., 2012). A counter argument states that Cr supplementation will not have such effects in all healthy people, but only in people with at least a moderate Cr deficiency. However, no accurate method for evaluating Cr status has been verified (DiSilvestro, 2005).
In people with diabetes, Cr supplementation has produced a decrease in fasting blood glucose values (Abdollahi et al., 2013;Bahijiri et al., 2000;Geohas et al., 2007;Pei et al., 2006;Rabinovitz et al., 2004). However, in other studies, Cr fails to affect blood glucose values (Abdollahi et al., 2013;Lee and Reasner, 1994;Preuss et al., 2000;Uusitupa et al., 1983). If Cr does indeed function as an essential nutrient, these variations could arise due to variations in the subjects' Cr status. If Cr acts solely as a drug, then the variations would depend on other factors. There have also been studies examining the effect of Cr on triglyceride levels in humans. A number of studies show a decrease in triglyceride values while also showing improved glucose control (Bahijiri et Geohas et al., 2007;Rabinovitz et al., 2004). These results reinforce an earlier proposed concept that Cr effects on lipid metabolism are mediated by effects on carbohydrate metabolism (Mertz, 1993). In contrast to this concept, in one human study, blood glucose readings are unchanged, but triglyceride values decrease (Lee and Reasner, 1994). Such a result suggests that Cr could affect lipid metabolism through either drug or nutritional mechanisms that go beyond modulation of carbohydrate metabolism. In this study lowering triglyceride but not glucose values, the study subjects had the following characteristics: a) Mainly a US Hispanic population, b) Diabetic people with fairly high fasting glucose values, c) People using insulin injections and/or oral glucoselowering drugs.
It needs to be determined if in other types of subjects, Cr supplementation can lower triglyceride concentrations without lowering glucose levels. Moreover, such additional studies should modify one protocol detail of the previous study. That study only compared post supplement values to post placebo values. New studies should also look at changes pre-to post-treatment with Cr or placebo. The present study did this for type 2 diabetic subjects who had the following characteristics: a) Live in the central Ohio area, b) Have just moderately elevated glucose levels, c) Do not take insulin injections.
This new study examined the same dose, intervention time, and Cr complex as the previous work where Cr affected readings for triglycerides, but not blood sugar (Lee and Reasner, 1994).

Subjects
The protocol was approved by The Ohio State University Human Conformance to these criteria was based on answers to an eligibility questionnaire.

Research design
Subjects were randomly assigned to one of two groups: Neither the subjects nor the person giving the supplements knew the group assignment. Subjects were instructed to maintain their previous dietary and exercise practices during participation. Subjects consumed the assigned product for 8 weeks and had blood samples taken before and after the supplementation period. Each sampling followed an overnight fast.

Blood analysis
Blood was collected by venipuncture into heparinized tubes. The tubes were centrifuged for approximately 10 min at 3000 rpm. Plasma was removed and stored at -70°C. Plasma glucose and triglycerides was assessed using the Roche Cobas C111 Clinical Chemistry Analyzer (Indianapolis, Indiana, USA). Table 1. Neither Cr supplementation nor placebo affected plasma glucose readings (Table 1). Cr supplementation, but not placebo, lowered plasma triglyceride values (Figure 1). The mean change in triglyceride values for the Cr group was significantly different than the change in the placebo group ( Figure 2

DISCUSSION
A number of studies in diabetic subjects have reported that Cr supplementation improves glucose status and decreases plasma triglycerides (Bahijiri et al., 2000;Geohas et al., 2007;Rabinovitz et al., 2004). However, not all studies on Cr supplementation in diabetic subjects show an effect on glucose status. Most of these studies do not look at triglyceride readings. On the other hand, one study has shown a decrease in triglyceride values without a change in blood glucose values (Lee and Reasner, 1994). This study was done in a US Hispanic type 2 diabetic population with fairly high fasting glucose values. Some of the subjects in the study had enough problem with controlling blood sugar that they were using insulin injections to try to treat the problem. The present study demonstrated that in subjects with moderately elevated blood glucose, and who were not using insulin injections, Cr supplementation can lower triglyceride concentrations without lowering glucose levels. The subjects studied here were recruited from a typical USA mid-western diabetic population. Thus, the results of this study expand on the previous study. Taken together, the two studies indicate that Cr supplementation can impact lipid metabolism via mechanisms not directly related to carbohydrate metabolism. Since plasma cholesterol concentrations were not changed by Cr, Cr would seem to affect this aspect of lipid metabolism via different mechanisms than the effects of triglyceride metabolism. Any Cr effects on cholesterol may be tied more directly to the effects on carbohydrate metabolism, which appeared to not occur in the present study. It is not yet certain why Cr supplementation impacts blood glucose related parameters in some studies but not others (DiSilvestro, 2005). Cr dose does not appear to be the only factor, though the dose used in the present study falls in the lower end of what has been used in diabetes studies (DiSilvestro, 2005). In the present study, the mean percent decrease in triglyceride values in the Cr picolinate group was not especially large (15%). However, in this study group, the mean initial triglyceride value was not extremely high. A larger decrease might occur when diabetics have a high starting blood triglyceride level. It is also possible that a higher Cr dose than used here might produce a bigger response.
It is not yet possible to determine whether the Cr dose used here worked by correcting some degree of deficiency or via a pharmacological effect. Unfortunately, a good means of assessing Cr status has not yet emerged (DiSilvestro, 2005). The present study's dose, 200 µg/day, is the upper end of what was established in 1989 as the estimated safe and adequate daily dietary intake range for Cr (National Research Council, Food and Nutrition Board, 1989 , 2001). Two, the possibility that diabetes raises Cr needs has not been ruled in or out yet. Third, if some degree of Cr deficiency does commonly exist in people with type 2 diabetes, a corrective action may temporarily require giving a dose above the normal requirement. Four, Cr has been suggested to not even be an essential nutrient for humans (Vincent, 2013). Lipidemia in diabetic populations presents a major risk for heart disease (Vijayaraghavan, 2010). Controlling triglyceride level with an inexpensive Cr supplement could decrease this risk. The current study showed that a Cr effect on triglycerides can occur without affecting blood glucose values, and without extreme conditions such as very high triglyceride readings or extremely poor glucose control.