International Journal of Plant Physiology and Biochemistry
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Article Number - 3F6AE37314


Vol.2(2), pp. 11-17 , May 2010

ISSN: 2141-2162



Full Length Research Paper

Study of inter-specific relationship in six species of Sesbania Scop. (Leguminosae) through RAPD and ISSR markers


Manoj Kumar Bisoyi1, Laxmikanta Acharya2, Arup Kumar Mukherjee3,4* and Chandra Panda Pratap2




 

1Department of Botany and Biotechnology, Khallikote Autonomous College, Berhampur, Orissa, India.

2Division of Taxonomy and Conservation, Regional Plant Resource Centre, Nayapalli, Bhubaneswar, Orissa, India.

3DNA Fingerprinting Laboratory, Division of Plant Biotechnology, Regional Plant Resource Centre, Nayapalli, Bhubaneswar, Orissa, India.

4Central Institute for Cotton Research, Shankar Nagar PO, Nagpur-440010, Maharashtra, India.

 

 


Email: titirtua@gmail.com






 Accepted: 08 March 2010  Published: 31 May 2010

Copyright © 2010 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


 

Six species of Sesbania were fingerprinted using RAPD and ISSR markers. Both the markers yielded a total of 249 bands out of which 243 were polymorphic in nature indicating high degrees of genetic diversity in the genus. Cluster analysis using the combined data revealed segregation of the lone species Sesbania grandiflora from the rest of the species. High boot strap values in the dendrograms show the accuracy and authenticity of the result. All other five species got separated to a distinct cluster. This supports the taxonomic division of the genus Sesbania into Agati Desv. comprising the only species S. grandiflora (Linn.) Poir. and sub-genus Eusesbania Baker containing all other species.

 

Key words: Sesbania, RAPD, ISSR, phenogram.


APA (2010). Study of inter-specific relationship in six species of Sesbania Scop. (Leguminosae) through RAPD and ISSR markers. International Journal of Plant Physiology and Biochemistry, 2(2), 11-17.
Chicago Manoj Kumar Bisoyi, Laxmikanta Acharya, Arup Kumar Mukherjee, and Chandra Panda Pratap. "Study of inter-specific relationship in six species of Sesbania Scop. (Leguminosae) through RAPD and ISSR markers." International Journal of Plant Physiology and Biochemistry 2, no. 2 (2010): 11-17.
MLA Manoj Kumar Bisoyi, et al. "Study of inter-specific relationship in six species of Sesbania Scop. (Leguminosae) through RAPD and ISSR markers." International Journal of Plant Physiology and Biochemistry 2.2 (2010): 11-17.
   
DOI
URL http://academicjournals.org/journal/IJPPB/article-abstract/3F6AE37314

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