Six species of Sesbania were fingerprinted using RAPD and ISSR markers. Both the markers yielded a total of 249 bands out of which 243 were polymorphic in nature indicating high degrees of genetic diversity in the genus. Cluster analysis using the combined data revealed segregation of the lone species Sesbania grandiflora from the rest of the species. High boot strap values in the dendrograms show the accuracy and authenticity of the result. All other five species got separated to a distinct cluster. This supports the taxonomic division of the genus Sesbania into Agati Desv. comprising the only species S. grandiflora (Linn.) Poir. and sub-genus Eusesbania Baker containing all other species.
Key words: Sesbania, RAPD, ISSR, phenogram.
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