International Journal of Plant Physiology and Biochemistry
Subscribe to IJPPB
Full Name*
Email Address*

Article Number - 48E45A552094


Vol.7(2), pp. 12-22 , April 2015
DOI: 10.5897/IJPPB2015.0227
ISSN: 2141-2162



Full Length Research Paper

Effect of cassava brown streak disease (CBSD) on cassava (Manihot esculenta Crantz) root storage components, starch quantities and starch quality properties



Nuwamanya Ephraim
  • Nuwamanya Ephraim
  • National Crops Resources Research Institute (NaCRRI), Root crops program, Biosciences Section, Kampala-Uganda.
  • Google Scholar
Baguma Yona
  • Baguma Yona
  • National Crops Resources Research Institute (NaCRRI), Root crops program, Biosciences Section, Kampala-Uganda.
  • Google Scholar
Atwijukire Evans
  • Atwijukire Evans
  • National Crops Resources Research Institute (NaCRRI), Root crops program, Biosciences Section, Kampala-Uganda.
  • Google Scholar
Acheng Sharon
  • Acheng Sharon
  • National Crops Resources Research Institute (NaCRRI), Root crops program, Biosciences Section, Kampala-Uganda.
  • Google Scholar
Alicai Titus
  • Alicai Titus
  • National Crops Resources Research Institute (NaCRRI), Root crops program, Biosciences Section, Kampala-Uganda.
  • Google Scholar







 Received: 13 February 2015  Accepted: 19 March 2015  Published: 29 April 2015

Copyright © 2015 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Effect of cassava brown streak disease on cassava root storage components were studied on four Ugandan varieties with varying levels of tolerance. Significant differences (P<0.05) were observed with reductions of 30% in amylose content and 50% in amylopectin content of diseased compared to healthy plots. Average dry matter content of diseased plots was 25% higher as much as starch yield and starch content reduced by 40 and 15% respectively in diseased plots compared to healthy plots. Susceptible varieties had lower protein and higher cyanide contents in diseased state compared to tolerant varieties. On pasting, mixed reactions were observed but importantly there were significant differences (P<0.05) in the starch pasting properties of starch from diseased compared to healthy plots. Plants with similar reactions to viral attack at the phenotypic level had different reactions when the levels of particular metabolite components (especially cyanide and starch constituents) were quantified.  The results point to hijacking of plant carbohydrate and nitrogen metabolic processes for viral metabolic gains. In turn, this affects the use of cassava for food and other applications but also points to possible use of metabolite based selections for tolerant varieties rather than mere root and stem phenotypic observations. 
 
Key words: Brown streak disease, Cassava, metabolism, starch, plant virus.

Alabi OJ, Kumar PL, Naidu, RA (2011). Cassava mosaic disease: A curse to food security in Sub-Saharan Africa. Online. APSnet Features.
 
Alicai T, Omongo CA, Maruthi MN, Hillocks RJ, Baguma Y, Kawuki R, Bua A, Otim-Nape GW, Colvin J (2007). Re-emergence of cassava brown streak disease in Uganda. Plant Dis. 91:24-29.
Crossref
 
Baguma Y, Sun C, Ahlandsberg S, Mutisya J, Palmqvist S, Rubaihayo PR, Magambo MJ, Egwang TG, Larsson H, Jansson C (2003). Expression pattern of the gene encoding starch branching enzyme II in storage root of cassava (Manihot esculenta Crantz). Plant Sci. 164:833-839.
Crossref
 
Benesi IR (2005). Characterization of Malawian cassava germplasm for diversity, starch extraction and its native and modified properties. PhD Thesis, Department of Plant Sciences, University of the Free State, South Africa pp. 74-123
 
Bradbury JH, Bradbury MG, Egan SV (1994). Comparison of Methods of analysis of cyanogens in cassava. Cassava Safety. Acta Horticulturae. 375:87-96.
 
Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
Crossref
 
Calvert LA, Thresh JM (2002). Viruses and Virus Diseases of Cassava. In Cassava: Biology, Production and Utilization; Hillocks, R.J., Thresh, J.M., Bellotti, A.C., Eds.; CABI Publishing: Oxon, UK. pp. 237-260.
Crossref
 
Dubois M, Gilles K, Hamilton K, Rebers A, Smith F (1956). Colorimetric Method for Determination of Sugars and Related Substances. Analytical Chem. 28:350-356.
Crossref
 
Fondong VNF, Pita S, Rey MEC, de Kochko A, Beachy RN, Fauquet CM (2000). Evidence of synergism between African cassava mosaic virus and a new double-recombinant geminivirus infecting cassava in Cameroon. J. Gen. Virol. 81:287-297.
PMid:10640569
 
Fraser RSS (1987). Biochemistry of Virus-Infected Plants. (Research Studies Press, Letchworth, Hartfordshire, UK).
 
Fu LJ, Shi K, Gu M, Zhou YH, Dong DK, Liang WS, Song FM,and and Yu JQ (2010). Systemic induction and role of mitochondrial alternative oxidase and nitric oxide in a compatible tomato-Tobacco mosaic virus interaction. Mol Plant Microbe Interact. 23(1):39-48.
Crossref
 
Goodman RN, Kiraly Z, and Wood KR. 1986. The Biochemistry and Physiology of Plant Disease. (University of Missouri Press, Columbia).
 
Handford MG, Carr JP (2006). Plant metabolism associated with resistance and susceptibility. In Natural Resistance Mechanisms of Plants to Viruses, pp. 315-340. Dordrecht: Springer.
Crossref
 
Haritatos E, Ayre BG, Turgeon R (2000). Identification of phloem involved in assimilate loading in leaves by the activity of the galactinol synthase promoter. Plant Physiol. 123:929-937
Crossref
 
Mbanzibwa DR, Tian YP, Tugume AK, Mukasa SB, Tairo F, Kyamanywa S, Kullaya A, and Valkonen JP (2009). Genetically distinct strains of Cassava brown streak virus in the Lake Victoria basin and the Indian Ocean coastal area of East Africa. Arch. Virol. 6:353-359.
Crossref
 
Morrison I, MIM, Asiedu EA, Stuchburry T, Powell AA (1995), Determination of lignin and Tannin content of cowpea seed coats. Annals of botany 76:287-290.
Crossref
 
Nuwamanya E, Baguma Y, Emmambux N, Taylor J, Patrick R (2010). Physicochemical and functional characteristics of cassava starch in Ugandan varieties and their progenies. J. Plant Breed. Crop Sci. 2:1-11
 
Odpio J, Ogwok E, Taylor NJ, Halsey M, Bua A, Fauquet CM and Alicai T (2013). Transgenic RNA interference (RNAi)-derived field resistance to cassava brown streak disease. Mol Plant Pathol 13:1019-31.
 
Ogwok E, Patil BL, Alicai T, Fauquet CM (2010). Transmission studies with Cassava brown streak Uganda virus (Potyviridae: Ipomovirus) and its interaction with abiotic and biotic factors in Nicotiana benthamiana. J. Virol. Methods. 6:296-304.
Crossref
 
Olesinski AA, Lucas WJ, Galun E, Wolf S (1995). Pleiotropic effects of tobacco-mosaic-virus movement protein on carbon metabolism in transgenic tobacco plants. Planta 197:118-126.
Crossref
 
Sajnani C, Zurita JZ, Roncel M, Ortega JM, Barón M, Ducruet JM (2007). Changes in photosynthetic metabolism induced by tobamovirus infection in Nicotiana benthamiana studied in vivo by chlorophyll thermoluminescence. New Phytologist, 175:120-130.
Crossref
 
Selman IW and SA Grant (2008). Some effects of nitrogen supply on the infection of tomato plants with tomato spotted wilt virus. Ann. Applied Biol., 45:448-455.
Crossref
 
Shalitin D and Wolf S (2000). Cucumber Mosaic Virus Infection Affects Sugar Transport in Melon Plants. Plant Physiol. 123(2):597-604
Crossref
 
Singh V, Shukla K (2009). Effect of PRSV infection on pigment content and assimilation of carbohydrate in Carica papaya L. Ann. Plant Prot. Sci. 17.
 
Sserubombwe WS, Briddon RW, Baguma YK, Ssemakula GN,Bull SE, Bua A, Stanley J (2008).Diversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Müll. Arg.) in Uganda. J. Gen. Virol. 89(7):1759-1769
Crossref
 
Tecsi LI, Maule AJ, Smith AM, Leegood RC (1996). A spatial analysis of physiological changes associated with infection of cotyledons of marrow plants with cucumber mosaic virus. Plant Physiol. 111:975-985.
PMid:12226342 PMCid:PMC160966
 
Watson MA, Watson DJ (1951). The effect of infection with beet yellows and beet mosaic viruses on the carbohydrate content of sugar-beet leaves, and on translocation. Annals Appl. Biol. 38(1):276-288.
Crossref

 


APA Nuwamanya E., Baguma Y., Atwijukire E., Acheng S., & Alicai T. (2015). Effect of cassava brown streak disease (CBSD) on cassava (Manihot esculenta Crantz) root storage components, starch quantities and starch quality properties. International Journal of Plant Physiology and Biochemistry, 7(2), 12-22.
Chicago Nuwamanya Ephraim, Baguma Yona, Atwijukire Evans, Acheng Sharon and Alicai Titus. "Effect of cassava brown streak disease (CBSD) on cassava (Manihot esculenta Crantz) root storage components, starch quantities and starch quality properties." International Journal of Plant Physiology and Biochemistry 7, no. 2 (2015): 12-22.
MLA Nuwamanya Ephraim, et al. "Effect of cassava brown streak disease (CBSD) on cassava (Manihot esculenta Crantz) root storage components, starch quantities and starch quality properties." International Journal of Plant Physiology and Biochemistry 7.2 (2015): 12-22.
   
DOI 10.5897/IJPPB2015.0227
URL http://academicjournals.org/journal/IJPPB/article-abstract/48E45A552094

Subscription Form