International Journal of Plant Physiology and Biochemistry
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Article Number - 72774CB309


Vol.2(1), pp. 6-10 , April 2010

ISSN: 2141-2162



Full Length Research Paper

Changes of some phenolic compounds and enzyme activities on infected pearl millet caused by Sclerospora graminicola


Kumar Arun*, P. C. Mali and V. K. Manga




Central Arid Zone Research Institute, Jodhpur-342003, India.


Email: arpurster@gmail.com






 Accepted: 30 November 2009  Published: 30 April 2010

Copyright © 2010 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


 

Purification of low abundance enzymes for biochemical characterization is frequently labor and cost-intensive. The existence of co-expressing multigene families increases the complexity of the procedure even further as it occurs with plant peroxidases where only the most abundant species have been studied. In this paper we present an optimized purification method for Zo peroxidase, a low abundance isoenzyme with unusual tolerance to hydrogen peroxide. This protocol is straightforward, allowing the purification of the enzyme at milligram levels in 10 days. Furthermore, the protocol may be easily adapted for the direct purification of other non abundant peroxidase isoenzymes from plant tissues.

 

Key words: Peroxidase, hydrogen peroxide, desactivation, Japanese radish.


APA (2010). Changes of some phenolic compounds and enzyme activities on infected pearl millet caused by Sclerospora graminicola. International Journal of Plant Physiology and Biochemistry, 2(1), 6-10.
Chicago Kumar Arun, P. C. Mali and V. K. Manga. "Changes of some phenolic compounds and enzyme activities on infected pearl millet caused by Sclerospora graminicola." International Journal of Plant Physiology and Biochemistry 2, no. 1 (2010): 6-10.
MLA Kumar Arun, P. C. Mali and V. K. Manga. "Changes of some phenolic compounds and enzyme activities on infected pearl millet caused by Sclerospora graminicola." International Journal of Plant Physiology and Biochemistry 2.1 (2010): 6-10.
   
DOI
URL http://academicjournals.org/journal/IJPPB/article-abstract/72774CB309

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