International Journal of Plant Physiology and Biochemistry
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Article Number - EE2C33E64910


Vol.9(3), pp. 22-35 , June 2017
DOI: 10.5897/IJPPB2017.0266
ISSN: 2141-2162



Full Length Research Paper

Identification of Pisum sativum L. cytokinin and auxin metabolic and signaling genes, and an analysis of their role in symbiotic nodule development



Dolgikh, E. A.
  • Dolgikh, E. A.
  • All-Russia Research Institute for Agricultural Microbiology, Podbelsky chausse 3, St. Petersburg, Pushkin, 196608, Russia
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Shaposhnikov, A. I.
  • Shaposhnikov, A. I.
  • All-Russia Research Institute for Agricultural Microbiology, Podbelsky chausse 3, St. Petersburg, Pushkin, 196608, Russia
  • Google Scholar
Dolgikh, A. V.
  • Dolgikh, A. V.
  • All-Russia Research Institute for Agricultural Microbiology, Podbelsky chausse 3, St. Petersburg, Pushkin, 196608, Russia
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Gribchenko, E. S.
  • Gribchenko, E. S.
  • All-Russia Research Institute for Agricultural Microbiology, Podbelsky chausse 3, St. Petersburg, Pushkin, 196608, Russia
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Bodyagina, K. B.
  • Bodyagina, K. B.
  • All-Russia Research Institute for Agricultural Microbiology, Podbelsky chausse 3, St. Petersburg, Pushkin, 196608, Russia
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Yuzhikhin, O. S.
  • Yuzhikhin, O. S.
  • All-Russia Research Institute for Agricultural Microbiology, Podbelsky chausse 3, St. Petersburg, Pushkin, 196608, Russia
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Tikhonovich, I. A.
  • Tikhonovich, I. A.
  • All-Russia Research Institute for Agricultural Microbiology, Podbelsky chausse 3, St. Petersburg, Pushkin, 196608, Russia
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 Received: 25 March 2017  Accepted: 31 May 2017  Published: 30 June 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The present work was designed to study the role of the phytohormones, cytokinin and auxin, in the symbiosis development between pea Pisum sativum L. and Rhizobium leguminosarum bv. viciae. To achieve this aim, the temporal expression patterns of the genes related to the cytokinin and auxin biosynthesis, perception, and response were studied. It was found that most of these genes showed specific transcriptional regulations upon nodulation at various stages following inoculation. Once they were purified using chromatographic methods, the endogenous cytokinins were detected by liquid chromatography-electrospray tandem mass spectrometry (LC[+]ES–MS). Our findings showed that cytokinin and auxin accumulation is tightly regulated during nodulation in order to control nodule organogenesis, infection events, and nodule function.

Key words: Legume-Rhizobium symbiosis, cytokinin, auxin, gene expression.

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APA Dolgikh, E. A., Shaposhnikov, A. I., Dolgikh, A. V., Gribchenko, E. S., Bodyagina, K. B., Yuzhikhin, O. S., & Tikhonovich, I. A. (2017). Identification of Pisum sativum L. cytokinin and auxin metabolic and signaling genes, and an analysis of their role in symbiotic nodule development. International Journal of Plant Physiology and Biochemistry, 9(3), 22-35.
Chicago Dolgikh, E. A., Shaposhnikov, A. I., Dolgikh, A. V., Gribchenko, E. S., Bodyagina, K. B., Yuzhikhin, O. S. and Tikhonovich, I. A.. "Identification of Pisum sativum L. cytokinin and auxin metabolic and signaling genes, and an analysis of their role in symbiotic nodule development." International Journal of Plant Physiology and Biochemistry 9, no. 3 (2017): 22-35.
MLA Dolgikh, et al. "Identification of Pisum sativum L. cytokinin and auxin metabolic and signaling genes, and an analysis of their role in symbiotic nodule development." International Journal of Plant Physiology and Biochemistry 9.3 (2017): 22-35.
   
DOI 10.5897/IJPPB2017.0266
URL http://academicjournals.org/journal/IJPPB/article-abstract/EE2C33E64910

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