International Journal of
Physical Sciences

  • Abbreviation: Int. J. Phys. Sci.
  • Language: English
  • ISSN: 1992-1950
  • DOI: 10.5897/IJPS
  • Start Year: 2006
  • Published Articles: 2464

Full Length Research Paper

Silver nanoparticles biogenic synthesized using an orange peel extract and their use as an anti-bacterial agent

Manal A. Awad
  • Manal A. Awad
  • King Abdullah Institute for Nanotechnology, King Saud University, Saudi Arabia.
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Awatif A. Hendi
  • Awatif A. Hendi
  • Department of Physics, Faculty of Science, King Saud University, Saudi Arabia.
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Khalid M. O. Ortashi
  • Khalid M. O. Ortashi
  • Department of Chemical Engineering, King Saud University, Saudi Arabia.
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Dalia F. A. Elradi
  • Dalia F. A. Elradi
  • Department of Microbiology, Faculty of Medicine, Princess Nora Bint Abdulrahman University. Saudi Arabia.
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Nada E. Eisa
  • Nada E. Eisa
  • Department of Physics, Al -Dammam University Kingdom of Saudi Arabia. Department of Physics, Omdurman Ahlia University, Sudan.
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Lamia. A. Al-lahieb
  • Lamia. A. Al-lahieb
  • Department of Chemistry Organic, Faculty of Science, Nature Product, Al Qassim University, Saudi Arabia.
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Shorog. M. Al-Otiby
  • Shorog. M. Al-Otiby
  • Department of Chemistry Organic, Faculty of Science, Nature Product, Princess Nora Bint Abdulrahman University, Saudi Arabia.
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Nada M. Merghani
  • Nada M. Merghani
  • Central Laboratory, College of Science, King Saud University, Saudi Arabia.
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Abdelelah A. G. Awad
  • Abdelelah A. G. Awad
  • Faculty of Animal Production, University of Khartoum, Sudan.
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  •  Accepted: 29 January 2014
  •  Published: 09 February 2014

Abstract

Synthesis of nanoparticles by green methods with antibacterial properties is of great researchers’ concern in the explored of new pharmaceutical and biomedical products. In this study, we synthesized a new product of nanosized particles of silver, non-toxic economy, clean, and conservator for energy. An environmentally friendly route is used for synthesizing silver nanoparticles (AgNPs) using an orange peel extract as both reducing and stabilizing agent at room temperature. The synthesized NPs were characterized using ultraviolet (UV)-Vis spectrophotometer, Zitasizer which measures the average size of the particles at about 91 nm, Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM) equipped with the energy dispersive spectroscopy (EDS) and characterization using Transmission electron microscopy (TEM). The results confirmed that the orange peel extract is a very good bioreductant for the synthesis of Ag NPs and we investigated the synthesized nanoparticles as an antibacterial which showed that the biogenic synthesized AgNPs exhibit inhibition, and had significant antibacterial against both gram-positive and gram-negative bacterial strains.

Key words: Silver nanoparticles, biogenic synthesis, orange peel, anti bacterial, gram-positive, gram-negative bacteria streamers.