ISABB Journal of Biotechnology and Bioinformatics
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Article Number - 9FB714744866


Vol.2(2), pp. 11-17 , October 2012

ISSN: 2163-9758



Effect of ascorbic acid on blackening and sprouting of Musa spp shoot tips


Chikezie U. N. Y.




Department of Biotechnology, Federal University of Technology, Owerri, P.M.B. 1526, Owerri, Imo State, Nigeria.


Email: uchikezie@yahoo.com






 Received: 06 January 2012  Accepted: 16 March 2012  Published: 30 October 2012

Copyright © 2012 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


 

      The effect of ascorbic acid (0, 5, 10, 20, 30, 40 and 150 mgl-1) on blackening and sprouting of Musa spp genotypes was investigated using shoot tip culture. Ascorbic acid did not minimize blackening, but rather markedly reduced the sprouting of cultured shoot tip explants. Ascorbic acid, genotype and genotype x ascorbic acid interactions were significant for shoot tip blackening and spouting, though genotype was a substantial source of variation to blackening response. ‘TMBX 5295 -1’ (cooking banana hybrid) shoot tips exhibited maximum blackening response, while ‘Agbagba’ (false-horn plantain) shoot tips showed highest sprouting. An inverse / negative relationship (r = - 0.27., P < 0.001) was obtained between shoot tip blackening and sprouting. Data obtained suggest that supplementation of medium supplemented (MS) medium with ascorbic acid was not beneficial for shoot tip culture of Musa spp. Other techniques for effective minimization of explant blackening are proposed.

 

Key words: Ascorbic acid, blackening, sprouting, Musa spp (banana and plantain) shoot tip culture.


APA Chikezie, U. N. Y. (2012). Effect of ascorbic acid on blackening and sprouting of Musa spp shoot tips. ISABB Journal of Biotechnology and Bioinformatics, 2(2), 11-17.
Chicago Chikezie U. N. Y.. "Effect of ascorbic acid on blackening and sprouting of Musa spp shoot tips." ISABB Journal of Biotechnology and Bioinformatics 2, no. 2 (2012): 11-17.
MLA Chikezie U. N. Y.. "Effect of ascorbic acid on blackening and sprouting of Musa spp shoot tips." ISABB Journal of Biotechnology and Bioinformatics 2.2 (2012): 11-17.
   
DOI
URL http://academicjournals.org/journal/ISABB-JBB/article-abstract/9FB714744866

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