September 2011

Investigation of the role of calcium in prolonging the shelf life of fluted pumpkin (Telefairia occidentalis Hook F.)

        A 16-week study using 2 certified land races of Telfairia occidentalis Hook F plants, to ascertain the effectiveness of calcium in prolonging the shelf life of the leaves and identify the pathogens that cause leaf rot of the plant was carried out. The two varieties were subjected to 5 levels of calcium carbonate treatments (0.25, 0.50, 0.75, and 1 g and control (0 g) in 20 kg...

Author(s): Akalazu J. N., Onyike, N. B., Offong, A. U. and Nwachukwu C. U.

September 2011

Protein engineering of yellow fluorescent protein insertion in outer membrane protein OmpF from Salmonella typhi

        Each monomer of the trimeric outer membrane porin OmpF of Salmonella typhi folds as a 16-stranded β-barrel with eight short turns at the periplasmic side and eight loops on the cell surface side. Yellow fluorescent protein (YFP) has been engineered into Loop 7 of OmpF. HindIII and EcoRV restriction sites were introduced in the region corresponding to Loop 7 of ompF gene....

Author(s): T. Madhuranayaki and S. Krishnaswamy