Journal of
Agricultural Biotechnology and Sustainable Development

  • Abbreviation: J. Agric. Biotech. Sustain. Dev.
  • Language: English
  • ISSN: 2141-2340
  • DOI: 10.5897/JABSD
  • Start Year: 2009
  • Published Articles: 112

Full Length Research Paper

Linkage mapping of lentil (Lens culinaris L.) genome using recombinant inbred lines revealed by AFLP, ISSR, RAPD and some morphologic markers

Bahattin Tanyolac1* Sehnaz Ozatay1 Abdullah Kahraman2 and Fred Muehlbauer3
  1Ege University, Department of Bioengineering, 35100 Bornova-Izmir Turkey. 2Harran University, Department of Field Crops, S. Urfa, Turkey. 3Department of Crop and Soil Sciences, USDA-ARS, Washington State University, 303 Johnson Hall, Pullman, WA99164-6434, USA.
Email: [email protected]

  • Article Number - 238348F549
  • Vol.2(1), pp. 1-6, January 2010
  •  Accepted: 09 December 2009
  •  Published: 31 January 2010

Abstract

 

We have constructed a molecular linkage map of lentil (Lens culinaris L.) in a RIL population (developed from the parents Precoz x WA8949041) of 94 plants with 166 markers. The resulting linkage map consists of 11 (varying between 50.9 – 436.5 cM) linkage groups covering 1396.3 cM with an average map distance between framework markers of 8.4 cM. Most of the RAPD and ISSR markers were evenly distributed across the genome. By using 40 primer combinations, we were able to generate 391 AFLP markers in the RIL population. The majority of the AFLP markers clustered in linkage group 1. In the segregation distortion analysis, 185 markers deviated from Mendelian segregation. Of the 185 markers with skewed segregation, 108 markers were unlinked and the rest of the distorted markers mapped to linkage groups. LG1 contained the most distorted marker among the linkage groups.

 

Key words: AFLP, RAPD, ISSR, lentil, segregation distortion, linkage map.

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