Journal of Agricultural Biotechnology and Sustainable Development
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Article Number - 238348F549


Vol.2(1), pp. 1-6 , January 2010

ISSN: 2141-2340



Full Length Research Paper

Linkage mapping of lentil (Lens culinaris L.) genome using recombinant inbred lines revealed by AFLP, ISSR, RAPD and some morphologic markers


Bahattin Tanyolac1* Sehnaz Ozatay1 Abdullah Kahraman2 and Fred Muehlbauer3




 

1Ege University, Department of Bioengineering, 35100 Bornova-Izmir Turkey.

2Harran University, Department of Field Crops, S. Urfa, Turkey.

3Department of Crop and Soil Sciences, USDA-ARS, Washington State University, 303 Johnson Hall, Pullman, WA99164-6434, USA.


Email: bahattin.tanyolac@ege.edu.tr






 Accepted: 09 December 2009  Published: 31 January 2010

Copyright © 2010 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


 

We have constructed a molecular linkage map of lentil (Lens culinaris L.) in a RIL population (developed from the parents Precoz x WA8949041) of 94 plants with 166 markers. The resulting linkage map consists of 11 (varying between 50.9 – 436.5 cM) linkage groups covering 1396.3 cM with an average map distance between framework markers of 8.4 cM. Most of the RAPD and ISSR markers were evenly distributed across the genome. By using 40 primer combinations, we were able to generate 391 AFLP markers in the RIL population. The majority of the AFLP markers clustered in linkage group 1. In the segregation distortion analysis, 185 markers deviated from Mendelian segregation. Of the 185 markers with skewed segregation, 108 markers were unlinked and the rest of the distorted markers mapped to linkage groups. LG1 contained the most distorted marker among the linkage groups.

 

Key words: AFLP, RAPD, ISSR, lentil, segregation distortion, linkage map.


APA (2010). Linkage mapping of lentil (Lens culinaris L.) genome using recombinant inbred lines revealed by AFLP, ISSR, RAPD and some morphologic markers. Journal of Agricultural Biotechnology and Sustainable Development, 2(1), 1-6.
Chicago Bahattin Tanyolac Sehnaz Ozatay Abdullah Kahraman and Fred Muehlbauer. "Linkage mapping of lentil (Lens culinaris L.) genome using recombinant inbred lines revealed by AFLP, ISSR, RAPD and some morphologic markers." Journal of Agricultural Biotechnology and Sustainable Development 2, no. 1 (2010): 1-6.
MLA Bahattin Tanyolac Sehnaz Ozatay Abdullah Kahraman and Fred Muehlbauer. "Linkage mapping of lentil (Lens culinaris L.) genome using recombinant inbred lines revealed by AFLP, ISSR, RAPD and some morphologic markers." Journal of Agricultural Biotechnology and Sustainable Development 2.1 (2010): 1-6.
   
DOI
URL http://academicjournals.org/journal/JABSD/article-abstract/238348F549

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