Journal of Agricultural Biotechnology and Sustainable Development
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Article Number - CD7DFD156070

Vol.10(3), pp. 34-44 , March 2018
ISSN: 2141-2340

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Full Length Research Paper

Hydroponic screening and characterization of aluminium tolerance on finger millet (Eleusine coracana (L.) Gaertn) accessions

Haftom Brhane
  • Haftom Brhane
  • Department of Biology, College of Natural and Computational Science, Haramaya University, Ethiopia.
  • Google Scholar
Edossa Fikru
  • Edossa Fikru
  • School of Agriculture, Medawelabu University, Ethiopia.
  • Google Scholar
Teklehaimanot Haileselassie
  • Teklehaimanot Haileselassie
  • Institite of Biotechnology, AAU, Addis Ababa, Ethiopia.
  • Google Scholar
Kassahun Tesfaye
  • Kassahun Tesfaye
  • Institite of Biotechnology, AAU, Addis Ababa, Ethiopia.
  • Google Scholar

 Received: 03 May 2017  Accepted: 10 January 2018  Published: 31 March 2018

Copyright © 2018 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0

Biotic and abiotic stress combined with the use of less productive local cultivars cause low production of finger millet in Ethiopia. This research was conducted to investigate acidity tolerance of finger millet accessions. Preliminary screening was done on 288 accessions and six improved national cultivars of finger millet. Twenty randomly selected and surface-sterilized seeds of each germplasm were wrapped and germinated in a tissue paper in Petri dishes. Thirty six hours-old seedlings of uniform size were transferred to the nutrient solutions having 500 µM KNO3, 500 µM CaCl2, 500 µM NH4NO3, 150 µM MgSO4.7H2O, 10 µM KH2PO4, 2 µM FeCl3 (III) and 112.5 µM Al2 (SO4)3.18H2O and allowed to grow for a further 8 days along with tolerant and susceptible references. Characterization with (112.5 µM) and without (0 µM) Al conditions was also done on 80 accessions. After eight days root and shoot length of seedlings were measured using a ruler, while fresh weight of these seedlings was taken using a digital balance. Mean separation and analysis of variance on each treatment was conducted using SPSS software. Relative total root length (RTRL) and root growth inhibition (RGI) were also estimated. From screening of 288 accessions, 75 (26.04%) of them were Al tolerant, while 213 (73.95%) of them were medium to susceptible. From characterization, 63 (78.75%) showed significant Al stress in root length, 23 (28.75%) in fresh weight, while no distinct and visible symptom were observed in shoot growth. The study clearly showed the possibility of developing lines and genotypes that can tolerate acidity in Ethiopian context and support agricultural development in acidic soils in the country and in the world.


Key words: Acid soils, finger millet, hydroponics, screening, plant roots.

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APA Brhane, H., Fikru, E., Haileselassie, T., & Tesfaye, K. (2018). Hydroponic screening and characterization of aluminium tolerance on finger millet (Eleusine coracana (L.) Gaertn) accessions. Journal of Agricultural Biotechnology and Sustainable Development, 10(3), 34-44.
Chicago Haftom Brhane, Edossa Fikru, Teklehaimanot Haileselassie and Kassahun Tesfaye  . "Hydroponic screening and characterization of aluminium tolerance on finger millet (Eleusine coracana (L.) Gaertn) accessions." Journal of Agricultural Biotechnology and Sustainable Development 10, no. 3 (2018): 34-44.
MLA Haftom Brhane, et al. "Hydroponic screening and characterization of aluminium tolerance on finger millet (Eleusine coracana (L.) Gaertn) accessions." Journal of Agricultural Biotechnology and Sustainable Development 10.3 (2018): 34-44.

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