Journal of
AIDS and HIV Research

  • Abbreviation: J. AIDS HIV Res.
  • Language: English
  • ISSN: 2141-2359
  • DOI: 10.5897/JAHR
  • Start Year: 2009
  • Published Articles: 258

Full Length Research Paper

Lysine and threonine plasma concentrations in Ivorian patients living with human immunodeficiency virus

Lydie Boyvin
  • Lydie Boyvin
  • Department of Clinical and Fundamental Biochemistry, Institut Pasteur of Côte d'Ivoire (IPCI) 01 BP 490, Abidjan, Côte d’Ivoire.
  • Google Scholar
Kipré Laurent Séri
  • Kipré Laurent Séri
  • Department of Clinical and Fundamental Biochemistry, Institut Pasteur of Côte d'Ivoire (IPCI) 01 BP 490, Abidjan, Côte d’Ivoire.
  • Google Scholar
Jeanne Armande Aké
  • Jeanne Armande Aké
  • Department of Clinical and Fundamental Biochemistry, Institut Pasteur of Côte d'Ivoire (IPCI) 01 BP 490, Abidjan, Côte d’Ivoire.
  • Google Scholar
Joseph Allico Djaman
  • Joseph Allico Djaman
  • Department of Clinical and Fundamental Biochemistry, Institut Pasteur of Côte d'Ivoire (IPCI) 01 BP 490, Abidjan, Côte d’Ivoire.
  • Google Scholar


  •  Received: 23 June 2017
  •  Accepted: 17 August 2017
  •  Published: 30 September 2017

Abstract

Côte d'Ivoire is one of the most affected countries in West Africa with HIV/AIDS, with a prevalence of 3.4%. Essential amino acids are needed by the organism as they play key roles in the immune system and they are supplied through diet. The objective of this study was to determine the plasma lysine and threonine status for better medical and nutritional management of patients living with HIV. This study involved 254 individuals: 127 HIV positive and 127 HIV negative (serving as controls) after confirmation of their HIV status through an HIV test (test DETERMINE® and GENIE II). Lysine and threonine were assayed using high performance liquid chromatography (HPLC) on plasma and CD4 lymphocyte count by the method of flow cytometry (FacsCalibur) from whole blood containing EDTA. This study showed that deficiency of lysine was more observed in male HIV infected individuals (66.7%) and threonine deficiency in female HIV infected individuals (17.1%) as compared to the controls subject. The amino acid concentrations as a measure of the degree of immunosuppression was significant for lysine (P = 0.0006) and not significant for threonine (P = 0.8640). The deficiency observed in HIV infected patients taking antiretrovirals is therefore probably due to viral infection and insufficient lysine intake in diet. The threonine concentration depends on the health condition of the subject.

Key words: Amino acids, essential, Côte d'Ivoire, HIV-infected patients, lysine, threonine.