African Journal of Bacteriology Research
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Article Number - 58BA6DC61451


Vol.8(4), pp. 22-28 , November 2016
DOI: 10.5897/JBR2016.0218
ISSN: 2006-9871



Full Length Research Paper

Antibiogram and the efficacy of leaf extract of Chromolena odorota (L.) R. M. King and H. Robinson on bacteria isolated from some swimming pools within Akure metropolis



Omoya, F. O.
  • Omoya, F. O.
  • Microbiology Department, Federal University of Technology, Akure, P. M. B. 704, Ondo State, Nigeria.
  • Google Scholar
Olukitibi, T. A.
  • Olukitibi, T. A.
  • Microbiology Department, Federal University of Technology, Akure, P. M. B. 704, Ondo State, Nigeria.
  • Google Scholar







 Received: 20 July 2016  Accepted: 29 August 2016  Published: 30 November 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Microorganisms being cosmopolitan are widely distributed in nature and can as well be found in swimming pools. These microorganisms contaminate swimming pools and other recreational water through indiscriminate defecation, contamination from rodents and birds etc. making these sources of water threat to human life. Concurrently, the resistant to conventional antibiotics has also increased the danger on people that are exposed to swimming pools, thus there is the need for alternative therapy. In view of this, study revealed the antibiogram of microorganisms isolated from swimming pools within Akure metropolis and the efficacy of Chromolena odorata as an alternative therapy to conventional antibiotics. The isolation, identification and antibiotic sensitivity of bacteria from selected swimming pools in Akure was carried out. Ethanol extract of the leaf of C. odorata was further tested on the isolates for comparative purpose with the commercial antibiotics using standard methods. The results of the experiment showed that the highest bacterial load of 7.10×103 cfu/ml was obtained for Swan hotel, while the least bacterial load was recorded for Sun view hotel with a bacterial load of 2.83×103 cfu/ml. Bacteria that were isolated and identified are Staphylococcus aureus, Proteus vulgaris, Staphylococcus epidermidis, Bacillus subtilis and Pseudomonas aeruginosa. The antibiotic sensitivity of the isolates showed that ofloxacin and ciprofloxacin were able to exert inhibitory effect on all the isolates. Most of the antibiotics were however resisted by P. aeruginosa, except ofloxacin. The inhibitory evaluation of ethanol extract of C. odorata on the bacterial isolates showed that the extract had its highest inhibitory effect on Proteus vulgaris with a zone diameter of 7.9 mm. The extract was however resisted by P. aeruginosa. These results showed that these swimming pools house a variety of microorganisms, some of which are pathogenic and should therefore be disinfected on regular basis to prevent dissemination of these bacteria by swimmers. C. odorata is also is a good alternative antimicrobial agent especially to P. vulgaris.

 

Key words: Chromolena odorata, Pseudomonas aeruginosa, Staphylococcus aureus, Proteus vulgaris, swimming pools.

Aho R, Hirn H (2001). A survey of fungi and some indicator bacteria in chlorinated water of indoor public swimming pools. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene B 173:242-249.

 

Ammara H, Salma R, Farah D, Shahid M (2009)Antimicrobial activity of some plant extracts having hepatoprotective effects J. Med. Plants Res. 3(1):20-23

 
 

Bartlett EC, Levison WB, Munday PE (2013). Pelvic inflammatory disease. BMJ 346:f3189.
Crossref

 
 

Beerepoot MA, TerRiet G, Nys S (2012). Lactobacilli vs antibiotics to prevent urinary tract infections: a randomized, double-blind, noninferiority trial in postmenopausal women. Arch. Intern. Med. 172(9):704-712.
Crossref

 
 

Bunyapraphatsara N, Chokechaijaroenporn O (2000). Thai medicinal plants. Faculty of Pharmacy, Mahidol University and National Center for Genetic Engineering and Biotechnology, Bangkok. 4:622-626.

 
 

Centre for Disease Control (2000). Pseudomonas dermatitis/folliculitis associated with pools and hot tubs – Colorado and Maine, 1999-2000. Morb. Mortal. Wkly. Rep. 49:1087-1091.

 
 

Centre for Disease Control (2001) Prevalence of parasites in fecal material from chlorinated swimming pools-United States. Morb. Mortal. Wkly. Rep. 50:410-412.

 
 

Donlan R (2002).Biofilms: microbial life on surfaces. Emerg. Infect. Dis. 8(9):881-890.
Crossref

 
 

GSRWE-Guidelines for Safe Recreational-water Environments (2000).Swimming Pools, Spas and Similar Recreational-water Environments. Final Draft for Consultation. 2:1-28.

 
 

Holt JG, Krieg NR, Sneath PH, Stanley JJ, Williams ST (1994) Bergey'sMannual of determinative Bacteriology. Wilkins Publishers. Baltimore.

 
 

Iwu MM, Duncan AR, Okunji CO (1999).New Antimicrobials of plant origin. In. Janick, Journal. (edition) Perspectives in New crops and New uses. ASHS Press, Alexandria, V.A. pp. 457-462.

 
 

Jayasingh BATD, Parkinson D (2008).Actinomycetes as antagonists of litter decomposer fungi. Appl. Soil Ecol. 38:109-118.
Crossref

 
 

Kumar Vinay, Abbas Abul K, Fausto Nelson, Mitchell Richard N. (2007).Robbins Basic Pathology (8th ed.). Saunders Elsevier. P 843.

 
 

Mbajiuka CS, Obeagu EI, Chude CN, Ihezie OE (2014). Antimirobial effects of Chromolaenaodorataon some human pathogens Int. J. Curr. Microbiol. Appl. Sci. 3(3):1006-1012

 
 

Nascimento GGF, Locatelli J, Freitas PC, Silva GL (2000).Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Braz. J. Microbiol. 31:247-256.
Crossref

 
 

Nostro A, Germano MP, D'Angelo A, Marino A, Cannatelli MA (2000). Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett. Appl. Microrobiol. 30(5):379-385.
Crossref

 
 

Rojas JJ, Ochoa VJ, Ocampo SA, Munoz JF (2006). Screening for antimicrobial activity of ten medicinal plants used in Colombian folkloric medicine: A possible alternative in the treatment of non-nosocomial infections. BMC Complement. Altern. Med. 6:2.
Crossref

 
 

Srisuda H, Suchada T, Piyaporn W, Niwat K, Sukhumaporn K (2016). Antimicrobial Activity of Chromolaenaodorata Extracts against Bacterial Human Skin Infections. Mod. Appl. Sci. 10(2):1-13.

 
 

Valarmathi S, Pandian RM, Senthilkumar B (2013). Incidence and screening of wound infection causing microorganisms. J. Acad. Ind. Res.1(8):508-510.

 
 

Vos T (2012). Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990-2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet 380(9859):2163-2196.
Crossref

 
 

World Health Organization (2008). Traditional medicine (Online).

 
 

Yoder J, Blackburn B, Craun G, Hill V, Levy D, Chen N (2004). Surveillance for waterborne-disease outbreaks associated with recreational water-United States, 2001-2002. MMWR Surveill. Summ. 53(SS-8):1-22.

 

 


APA Omoya, F. O., & Olukitibi, T. A. (2016). Antibiogram and the efficacy of leaf extract of Chromolena odorota (L.) R. M. King and H. Robinson on bacteria isolated from some swimming pools within Akure metropolis. African Journal of Bacteriology Research, 8(4), 22-28.
Chicago Omoya, F. O. and Olukitibi, T. A.. "Antibiogram and the efficacy of leaf extract of Chromolena odorota (L.) R. M. King and H. Robinson on bacteria isolated from some swimming pools within Akure metropolis." African Journal of Bacteriology Research 8, no. 4 (2016): 22-28.
MLA Omoya, et al. "Antibiogram and the efficacy of leaf extract of Chromolena odorota (L.) R. M. King and H. Robinson on bacteria isolated from some swimming pools within Akure metropolis." African Journal of Bacteriology Research 8.4 (2016): 22-28.
   
DOI 10.5897/JBR2016.0218
URL http://academicjournals.org/journal/JBR/article-abstract/58BA6DC61451

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