African Journal of Bacteriology Research
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Article Number - 9C967B357963


Vol.8(2), pp. 8-13 , February 2016
DOI: 10.5897/JBR2015.0162
ISSN: 2006-9871



Full Length Research Paper

Phenotypic and genotypic analysis of vancomycin-resistant Enterococci strains isolated from different water sources



Jie Zhang
  • Jie Zhang
  • College of Animal Science, Fujian Agriculture and Forestry University, Fu Zhou, Fujian Province 350002, China
  • Google Scholar
Yan-Qiu Wang
  • Yan-Qiu Wang
  • College of Animal Science, Fujian Agriculture and Forestry University, Fu Zhou, Fujian Province 350002, China
  • Google Scholar
Jian Li
  • Jian Li
  • College of Animal Science, Fujian Agriculture and Forestry University, Fu Zhou, Fujian Province 350002, China
  • Google Scholar
Yu-Fang Ma
  • Yu-Fang Ma
  • College of Animal Science, Fujian Agriculture and Forestry University, Fu Zhou, Fujian Province 350002, China
  • Google Scholar
Jin Lin
  • Jin Lin
  • College of Animal Science, Fujian Agriculture and Forestry University, Fu Zhou, Fujian Province 350002, China
  • Google Scholar
Zhen-Run Xiao
  • Zhen-Run Xiao
  • College of Animal Science, Fujian Agriculture and Forestry University, Fu Zhou, Fujian Province 350002, China
  • Google Scholar
Dao-Jin Yu
  • Dao-Jin Yu
  • College of Animal Science, Fujian Agriculture and Forestry University, Fu Zhou, Fujian Province 350002, China
  • Google Scholar







 Received: 04 April 2015  Accepted: 16 October 2015  Published: 29 February 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The purpose of this study was to investigate the phenotype, genotype and relationships of vancomycin-resistant Enterococcib (VRE) strains isolated from Minjiang River of Fujian province, residence district pond, university fish farm, cesspool and sewer. PCR was used to confirm Enterococci and amplify antibiotic resistance genes. Relationships between different strains were determined by repetitive extragenic palindromic elements-PCR (REP-PCR). A total of 17 VRE strains were isolated from water environment, among which 9 strains were isolated from cesspool, 6 strains were isolated from sewer and 2 strains were isolated from university fish farm. VRE was not isolated from Minjiang River or residence district pond. Analysis on the antibiotic resistance gene showed that 3 strains belong to genotype VanB, 9 strains belong to VanC1 and 5 strains belong to genotype VanC2/3. Genotype VanA was not detected. REP-PCR analysis showed that 17 strains were divided into type A1 (n=6), type A2 (n=1), type A3 (n=2), type B (n=3), type C (n=1), type D (n=3) and type E (n=1). These results demonstrated that the antibiotic resistant phenotype was consistent with the genotype of 17 VRE strains. VRE strains isolated from water sources were resistant to multiple antibiotics. The majority of the strains isolated in this study shared high similarity.

 

Key words: Enterococci, antibiotic resistance, vancomycin, repetitive extragenic palindromic elements-PCR

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APA Zhang, J., Wang, Y., Li, J., Ma, Y., Lin, J., Xiao, Z., & Yu, D. (2016). Phenotypic and genotypic analysis of vancomycin-resistant Enterococci strains isolated from different water sources. African Journal of Bacteriology Research, 8(2), 8-13.
Chicago Jie Zhang, Yan-Qiu Wang, Jian Li, Yu-Fang Ma, Jin Lin, Zhen-Run Xiao and Dao-Jin Yu. "Phenotypic and genotypic analysis of vancomycin-resistant Enterococci strains isolated from different water sources." African Journal of Bacteriology Research 8, no. 2 (2016): 8-13.
MLA Jie Zhang, et al. "Phenotypic and genotypic analysis of vancomycin-resistant Enterococci strains isolated from different water sources." African Journal of Bacteriology Research 8.2 (2016): 8-13.
   
DOI 10.5897/JBR2015.0162
URL http://academicjournals.org/journal/JBR/article-abstract/9C967B357963

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