Vibrio cholera is a Gram negative contagious water borne bacteria which is responsible for cholera outbreak all over the world. The disease spread occurs much more during summers in tropical and endemic regions. Entering dangerous waste to surface waters is a serious environmental health problem in developing countries. The aim of this study is to investigate the surface waters contamination with V. cholera in Boyer-Ahmad area. This cross-sectional study was performed during six months 2013 in Boyer-Ahmad distinct. One hundred and twenty water samples were collected from 10 stations during 6 months, totally 120 samples which were collected and after centrifugation, sediments were collected and cultured in Tryptic Soy Broth and Thiosulphate Citrate Bile Sucrose agar (TCBS). Biochemical diagnostic tests performed and polymerase chain reaction (PCR) were used for more detection sensitivity. None of the suspected isolates in bacterial cultures have been confirmed as V. cholera. The results are concordance to health centers in the region where no cholera cases were reported during the year. However, periodic surveillance of water contamination by contagious agents has been highly recommended.

Keywords: Vibrio cholera, environmental surveillance, surface water, contamination.