Journal of Cell and Animal Biology
Subscribe to JCAB
Full Name*
Email Address*

Article Number - 0A7D89058852


Vol.10(2), pp. 9-15 , May 2016
DOI: 10.5897/JCAB2016.0444
ISSN: 1996-0867



Full Length Research Paper

Characterization and anti-tumor effect of shark liver extraction



Yuqin Xi
  • Yuqin Xi
  • College of Life Sciences, Northwest Normal University, Lanzhou, 730070. Gansu, China.
  • Google Scholar
Yunwang Zhao
  • Yunwang Zhao
  • College of Life Sciences, Northwest Normal University, Lanzhou, 730070. Gansu, China.
  • Google Scholar
Chunyan Ren
  • Chunyan Ren
  • College of Life Sciences, Northwest Normal University, Lanzhou, 730070. Gansu, China.
  • Google Scholar







 Received: 06 March 2016  Accepted: 19 May 2016  Published: 31 May 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Shark liver oil (SLO) has been widely used as a food supplement to boost human immunity. The extraction method of shark liver oil is immature. In this study, four different extraction methods of shark liver oil were tried, the effect of the solid-liquid ratio, quality of the enzyme, reaction pH and temperature on SLO extraction rate have been discussed. Infrared spectroscopy, gas chromatography and high performance liquid chromatography was used to detect the main composition of SLO, and U14 tumor bearing mice were used to explore anti-tumor effect of SLO. Our results show that alkaline protease extraction method was the best with an extraction rate of 30.15%, and the solid-liquid ratio, quality of the enzyme, reaction pH and temperature was 1:2, 2% of the liver quality, 9, 3.5 h, 48°C respectively. Characteristic peak of alkoxy glycerol, squalene and vitamin A was presented in the IR spectra of SLO, the proportion of alkoxy glycerol, squalene content and vitamin was 35.3, 40.4 and 7.8% determined by gas chromatography (GC) and high performance liquid chromatography (HPLC). The inhibition rate was 54.56.

Key words: Shark liver oil, extraction, characterization, gas chromatography, high performance liquid chromatography (HPLC).

Bao D, Tao NP, Liu M (2006). Analysis gem fish oil extraction, refining and fatty acid composition. Food Sci. 27:169-172.

 

Chen N, Yang Y (2014). Study on the method of detecting the glycerol in shark liver alcohol by infrared spectroscopy. North Pharmaceut. 11(12):23.

 
 

Dai Y (2009). Research on Technology of Separation and Purification of Squalene from Olive Oil. Tianjin University.

 
 

Han Y, Shui X, Jiang X (2003). Supercritical CO2 extraction of shark liver oil. Chin. Oil 10:33-35.

 
 

Hu A, Qiu T (2002). Ultrasound technology in the food industry, Acoust. Technol. (2):192-194.

 
 

Huang ZR, Lin YK, Fang JY (2009). Biological and pharmacological activities of squalene and related compounds: Potential uses in cosmetic dermatology. Mol. 14:540-554.
Crossref

 
 

Joon-Soo S, A-Rang I, Seung MC, Hae JJ, Jin HJ, Yeong SK (2007). Evaluation of chondroitin sulfate in shark cartilage powder as a dietary supplement: Raw materials and finished products. Food Chem. 101(2):532-539.
Crossref

 
 

Laurence E, John C, Angus MN (2002). Potential of squalene as a functional lipid in foods and cosmetics. Lipid Technol. 12:104-107.

 
 

Ma YJ, Yang B (2010). World Marine fish resources utilization situation and development trend, Chin. Oil 35:1-3.

 
 

Qi D (2010). Research on Separation and Extraction of Squalene from SODD, "College of chemical engineering, Tianjin University".

 
 

Romain M, Cheminade C, Allaume P, Legrand P, Legrand AB (2004).Oral intake of shark liver oil modifies lipid composition and improves motility and velocity of boar sperm. Theriogenol. 62(8):1557-1566.
Crossref

 
 

Tian X (2011). Establishment and application of analutical methods of the squalene and alkylglycerols in shark liver oil. South China University.

 
 

Wang L (2003). Analysis of Alkoxyglycerols Components in Shark Liver Oil. J. Wuxi Univ. Light Ind. 23(2):90-93.

 
 

Wang C (2003). Progress oncogenes, tumor suppressor genes, proliferating cell nuclear antigen and ovarian cancer. Prognosi. Med. Summ. 9(28):30.

 
 

Wang F (2006). Enrichment of fish oil polyunsaturated fatty acids. Tianjin University.

 
 

Zi Y, Bai S (2000). Shark liver oil - the patron saint of modern health. Food Health 5:10-11.

 

 


APA Xi, Y., Zhao, Y., & Ren, C. (2016). Characterization and anti-tumor effect of shark liver extraction. Journal of Cell and Animal Biology, 10(2), 9-15.
Chicago Yuqin Xi, Yunwang Zhao and Chunyan Ren. "Characterization and anti-tumor effect of shark liver extraction." Journal of Cell and Animal Biology 10, no. 2 (2016): 9-15.
MLA Yuqin Xi, Yunwang Zhao and Chunyan Ren. "Characterization and anti-tumor effect of shark liver extraction." Journal of Cell and Animal Biology 10.2 (2016): 9-15.
   
DOI 10.5897/JCAB2016.0444
URL http://academicjournals.org/journal/JCAB/article-abstract/0A7D89058852

Subscription Form