Journal of Cell and Animal Biology
Subscribe to JCAB
Full Name*
Email Address*

Article Number - 29701AF62093

Vol.10(4), pp. 22-29 , December 2016
DOI: 10.5897/JCAB2016.0449
ISSN: 1996-0867

Full Length Research Paper

Sheep and goats pasteurellosis: Isolation, identification, biochemical characterization and prevalence determination in Fogera Woreda, Ethiopia

Tewodros Alemneh*
  • Tewodros Alemneh*
  • Woreta City Office of Agriculture and Environmental Protection, S/Gondar, Woreta, Ethiopia.
  • Google Scholar
Annania Tewodros
  • Annania Tewodros
  • Faculty of Veterinary Medicine, University of Gondar, P.O. Box: 196, Gondar, Ethiopia.
  • Google Scholar

 Received: 24 June 2016  Accepted: 18 October 2016  Published: 31 December 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0

A cross sectional study was carried out on pasteurellosis of small ruminants in Fogera Woreda, Ethiopia. The objectives of this research were isolation and characterization of the bacterial species by culture and biochemical testes, and determining the prevalence in apparently pneumonic small ruminants. Out of the total 988 samples examined, 322 were detected positive for pasteurella with an overall infection rate of 32.6%. Of which, 180 (55.9%) were from nasal swabs and 142 (44.1%) were from blood samples. Accordingly, 79.5% of the isolates were Mannheimia haemolytica and 20.5% were Pasteurella multocida. Significantly (p<0.05) higher prevalence was detected in sheep (37.1%), females (36.42%), young (52.97%), and extensively managed ruminants (38.15%) than those in goats (21.9%), males (25.29%), adults (21.26%) and semi-intensive production systems (17.18%), respectively. Similarly, the frequency of infection was significantly (p<0.05) higher in winter (48.6%) and spring (32.85%) as compared to autumn (23.79%) and summer (19.67%). In conclusion, this finding showed that the disease is highly prevalent in the study area. Thus, an integrated application of vaccination and overall management measures should be implemented to prevent and control the disease in animals.

Key words: Pasteurellosis, Isolation, Mannheimia haemolytica, Pasteurella multocida, Sheep and Goats, Identification, Fogera, Ethiopia.

Abera D, Sisay T, Birhanu T (2014). Isolation and Identification of Mannheimia and Pasteurella species from pneumonic and apparently healthy cattle and their antibiogram susceptibility pattern in Bedelle District, Western Ethiopia. Afr. J. Bacteriol. Res. 6(5):32-41.


Aiello SE, May A (1998). The Merck Veterinary Manual. 8th edition. New Jersey. USA: Merck and Co, Inc; pp. 1053-1057.


Alemu S, Esayas GL, Gelagay G, Aschalew Z (2006). Study on Small ruminant lungworm in Northeastern Ethiopia. Vet. Parasitol. 142(3):330-335.


Amhara Regional State Bureau of Agriculture and Rural Development (ARSBARD) (2006). Annual Regional Agricultural Report.


Aschalew Z (1998). A study on Pneumonic Pasteurellosis in North Shewa. Debre Zeit: DVM Thesis. AAU, FVM. pp. 1-59.


Asefa DA, Lubke B, Laikemariam Y, Yilikal A, Shimmel DH, Terefe G (2001). Epidemiology and strain characterization of ovine Pasteurellosis in selected sites of Ethiopia. Sheno Agricultural Research Center, Debre Berhan, Ethiopia.


Baundreaux CM (2004). A novel strategy of controlling bovine pneumonic pasteurellosis transfecting the upper respiratory tract of cattle with a gene coding for the antimicrobial peptide cecropin. MSc Thesis, Louisiana state University, USA.


Biberstin L, Hirsh C (1999). Pasteurella. In: Veterinary Microbiology. Black science Inc., USA. pp. 135-139.


Carter GR (1984a). Diagnostic procedures in Veterinary Bacteriology and Mycology. Charles. 4th edition. Thomas, USA. Pp. 3-19.


Carter GR (1984b). Isolation and identification of bacteria from clinical specimens. In Diagnostic Procedures in Veterinary Bacteriology and Mycology. 4th ed. Edited by Charles C. USA: Thomas; pp.19-30.


Center for Statistical Agency (CSA) (2008). Federal Democratic Republic of Ethiopia, Center for Statistical Agency, agricultural sample survey 2008/09, statistical bulletin 446 p.


Central Statistical Agency (CSA) (2009). The Federal Democratic Republic of Ethiopia central statistical agency, agriculture in figure key findings of the 2008/9-2010/11 agricultural sample surveys for all sectors and seasons country summary, FDRECSA Addis Ababa, Ethiopia. pp. 14-18.


Craig TM (1994). Epidemiology of internal parasite, effect of climate and host on reproductive cycle in parasite survival. In: small ruminants for the mixed animal practitioner, western veterinary conference, Las Vegas, Nevada.


Daniel JA, Held JE, Boake DG, Wulf DM, Epperson WB (2006). Evaluation of the prevalence and onset of lung lesions and their impact on growth of lambs. Am. J. Vet. Res. 67:890-894.


Eshetu M (1991). Pneumonic Pasteurellosis in sheep slaughtered at Addis Ababa abattoir. Debre Zeit: DVM Thesis, FVM, AAU.


Fogera Woreda Office of Agriculture and Rural Development (FWOARD) (2004). Animal Population Estimates in Fogera Woreda, Amhara Regional State, Ethiopia.


Gatenby RM (1999). Sheep In: The tropical agriculturalist. Basin stock, London.


Gilmour NJ Gilmour JS (1989). Pasteurellosis of sheep. London: Adlam and Rutter; pp. 223-262.


Jones TC, Hunt RD, King MW (1997). Pasteurella multocida pneumonia seminar in Respiratory infections. 12:54-56.


Lobago F, Bekana B, Gustafsso H, Kindahl H (2006). Reproductive performance of dairy cows in small holder production system in Selalle, central Ethiopia. Trop. Anim. Prod. Health 38:333-342.


MacFadinn JF (2000). Biochemical tests for identification of medical bacteria. 3rd ed. New York: Williams and Wilkins Lippincott; ISBN: 0683,05318-05183.


Maru H, Takele, Adem H (2013). A study on Ovine pneumonic Pasteurellosis: Isolation and Identification of Pasteurellae and their antibiogram susceptibility pattern in Haramaya District, Eastern Hararghe, Ethiopia. BMC Vet. Res. 9:239.


Mohamed RA, Abdelsalam EB (2008). A review on pneumonic pasteurellosis (respiratory mannhaeimiosis) with emphasis on pathogenesis, virulent mechanisms and pre-disposing factors. Bulg. J. Vet. Med. 11(3):139-160.


Mohammed R (1999). Bacteriological and histological examination of pneumonic lungs of small ruminants slaughtered at HashimNure export abattoir. Debre Zeit: DVM Thesis, FVM, AAU. P 21.


Quinn PJ, Carter ME, Markey B, Carter GR (1994). Bacterial pathogens: microscopy, culture and identification. In Clinical Veterinary Microbiology. London, England: Wolfe publishing; pp. 20-60.


Quninn PJ, Markey BK, Carter ME, Donnelly WJ, Leonard FC (2002). Veterinary Microbiology and Microbial Diseases. 1st ed. Blackwell Science, USA. pp. 137-143.


Radostits OM, Gay C, Blood DC, Hinchclift KW (2000). Diseases the Respiratory system. In: Veterinary Medicine, a text book of the disease of cattle, sheep, pigs, goats and horse. 9th ed. Saunders, London. pp. 1550-1600.


Rasha M, Othman H, Ibraheim K, Moaed SH (2014). Conventional and Molecular Detection of Pasteurella multocida in Outbreak of Respiratory Tract Infection of Sheep and Goats in Basrah Province, Iraq.Bas.J. Vet. Res. 1:1.


Sharma SN, Adlakha SC (1996). Text book of veterinary Microbiology. Masjid: Vikas publishing house PVT LTD.


Tesfaye S (1997). Serological and bacteriological investigation of P. haemolytica serotypes in sheep in the high lands of Wollo, North Eastern Ethiopia. Debre Zeit: DVM Thesis, AAU, FVM; 65 p.


Tewodros AE, Dawit AA (2015). Sero-Prevalence of Small Ruminant Brucellosis in and around Kombolcha, Amhara Regional State, North-Eastern Ethiopia. J. Vet. Sci. Med. Diagn. 4:5.


Thrusfield M (2005). Veterinary Epidemiology. 3rd ed. Black well science, Ltd., London pp. 228-246.


Tilaye D (2010). Pathological, bacteriological and serological study on pneumonic sheep and goats with special emphasis on Mannheimia, Babersteinia, Pasteurella and Mycoplasma species. MSc Thesis. AAU, FVM, Debre Zeit, Ethiopia. pp. 1-97.


Wilsmore T (2006). Diseases of small ruminants in Ethiopia, the veterinary epidemiology and economics research unit school of agriculture's policy and development the university of read, UK. Pp. 67-72.


Yeshwas F, Shigdaf M, Hailu M, Agraw A (2013). Serotyping and Evaluation of the Level of Protective Antibody Titer in North-West Ethiopian Sheep Before and After Ovine Pasteurellosis Vaccination. Int. J. Pharma Med. Biol. Sci. pp. 1-8.


Zewdie S (2004). Current status of veterinary services in Ethiopia. In: Proceedings of a national work shop on managing animal health constraint to export marketing of meat and livestock.


APA Alemneh, T., & Tewodros, A. (2016). Sheep and goats pasteurellosis: Isolation, identification, biochemical characterization and prevalence determination in Fogera Woreda, Ethiopia. Journal of Cell and Animal Biology, 10(4), 22-29.
Chicago Tewodros Alemneh and Annania Tewodros. "Sheep and goats pasteurellosis: Isolation, identification, biochemical characterization and prevalence determination in Fogera Woreda, Ethiopia." Journal of Cell and Animal Biology 10, no. 4 (2016): 22-29.
MLA Tewodros Alemneh and Annania Tewodros. "Sheep and goats pasteurellosis: Isolation, identification, biochemical characterization and prevalence determination in Fogera Woreda, Ethiopia." Journal of Cell and Animal Biology 10.4 (2016): 22-29.
DOI 10.5897/JCAB2016.0449

Subscription Form