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Article Number - 381F7A49804


Vol.5(1), pp. 1-5 , April 2013
DOI: 10.5897/JCBBR12.012
ISSN: 2141-2227



Full Length Research Paper

MLH1 gene: An in silico analysis


Amitha Joy*, Jubil, C. A, Syama, P. S and Rohini Menon




Department of Biotechnology, Sahrdaya college of Engineering and Technology, Kodakara-680684, Thrissur, India.


Email: amithajoy@sahrdaya.ac.in






 Accepted: 07 February 2013  Published: 30 April 2013

Copyright © 2013 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The MLH1 gene responsible for colon cancer has been examined to identify functional consequences of single-nucleotide polymorphisms (SNPs). 16 SNPs have been identified in the MLH1 gene in which all are found to be nonsynonymous. Non synymous SNPs are relevant in many of the human inherited diseases since they change the aminoacid sequence of the protein. 56% of the identified nsSNPs have been reported as damaging. In the analysis of SNPs using SIFT, UTRscan, FastSNP and PolyPhen-2, it was recognized that rs41295284 and rs35001569 were responsible for the alteration in levels of expression. It has been concluded that among all SNPs of MLH1 gene, the mutation in rs41295284 and rs35001569 have the most significant effect on functional variation.

 

Key words: Single nucleotide polymorphism, non-synonymous, colon cancer.

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APA (2013). MLH1 gene: An in silico analysis. Journal of Computational Biology and Bioinformatics Research, 5(1), 1-5.
Chicago Amitha Joy, Jubil, C. A, Syama, P. S and Rohini Menon. "MLH1 gene: An in silico analysis." Journal of Computational Biology and Bioinformatics Research 5, no. 1 (2013): 1-5.
MLA Amitha Joy, et al. "MLH1 gene: An in silico analysis." Journal of Computational Biology and Bioinformatics Research 5.1 (2013): 1-5.
   
DOI 10.5897/JCBBR12.012
URL http://academicjournals.org/journal/JCBBR/article-abstract/381F7A49804

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