Journal of Cell Biology and Genetics
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Article Number - 3F6D6CD909


Vol.2(1), pp. 1 - 7 , March 2011

ISSN: 2141-6516



Full Length Research Paper

Overexpression of Arabidopsis PP2CA2 gene shows phenotypes related to abscisic acid responses



Guo Xinhong
  • Guo Xinhong
  • College of Life Sciences, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, P. R. China.
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Cui Yi
  • Cui Yi
  • College of Life Sciences, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, P. R. China.
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Wang Jie
  • Wang Jie
  • College of Life Sciences, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, P. R. China.
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Zhao Qiong
  • Zhao Qiong
  • College of Life Sciences, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, P. R. China.
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Hu Shuai
  • Hu Shuai
  • College of Life Sciences, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, P. R. China.
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Tang Dongying
  • Tang Dongying
  • College of Life Sciences, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, P. R. China.
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Liu Xuanming
  • Liu Xuanming
  • College of Life Sciences, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, P. R. China.
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 Accepted: 03 September 2010  Published: 30 March 2011

Copyright © 2011 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


In this study we overexpressed PP2CA2 gene in Arabidopsis to provide genetic evidence on this gene function in seed germination and other plant responses. The average seed germination percentage of constitutive expression of AtPP2CA2 under a cauliflower mosaic virus 35S promoter (35S::AtPP2CA2) was lower than that of wild type under 0.3 and 1 uM exogenous abscisic acid (ABA), indicating an increased sensitivity of 35S::AtPP2CA2 seeds to ABA with respect to wild type. The35S::AtPP2CA2 seedlings showed an increased inhibition of root growth compared with wild-type plants. In the absence of exogenous ABA, the water loss in35S::AtPP2CA2 plants was lower than that in wild type. There is no obvious difference of water loss between 35S::AtPP2CA2 and wild type plants under 10 uM ABA treatment. The 35S::AtPP2CA2 plants had smaller stomatal apertures than did wild-type plants under ABA treatment. Furthermore, the 35S::AtPP2CA2 seedlings showed a reduced inhibition of hypocotyl growth compared with wild-type plants under the 0, 10, 20, 30 and 40 uM exogenous ABA. The study on overexpression of AtPP2CA2gene could provide an approach for improving crop performance under stress conditions.

 

Key words: Arabidopsis, AtPP2CA2 gene, phenotype, abscisic acid signal transduction.

 

Camilleri C, Azimzadeh J, Pastuglia M, Bellini C, Grandjean O, Bouchez D (2002). The Arabidopsis TONNEAU2 gene encodes a putative novel protein phosphatase 2A regulatory subunit essential for the control of the cortical cytoskeleton. Plant Cell, 14: 833-845.
http://dx.doi.org/10.1105/tpc.010402
PMid:11971138 PMCid:PMC150685
 
Clough SJ, Bent AF (1998). Floral dip: a simplified method for Agrobacterium- mediated transformation of Arabidopsis thaliana. Plant J., 16: 735-743.
http://dx.doi.org/10.1046/j.1365-313x.1998.00343.x
PMid:10069079
 
Cohen PT (1997) Novel protein serine/threonine phosphatases: variety is the spice of life. Trends Biochem. Sci., 22: 245-251.
http://dx.doi.org/10.1016/S0968-0004(97)01060-8
 
Cutler SR, Rodriguez PL, Finkelstein RR, Abrams SR (2010). Abscisic acid: emergence of a core signaling network. Annu. Rev. Plant Biol., 61: 651-679.
http://dx.doi.org/10.1146/annurev-arplant-042809-112122
PMid:20192755
 
DeLong A (2006) Switching the flip: protein phosphatase roles in signaling pathways. Curr. Opin. Plant Biol., 9: 470-477.
http://dx.doi.org/10.1016/j.pbi.2006.07.015
PMid:16890477
 
Fujii H, Chinnusamy V, Rodrigues A, Rubio S, Antoni R, Park SY, Cutler SR, Sheen J, Rodriguez PL, Zhu JK (2010). In vitro reconstitution of an abscisic acid signalling pathway. Nature, 462(7273):660-664.
http://dx.doi.org/10.1038/nature08599
PMid:19924127 PMCid:PMC2803041
 
Gonzalez-Garcia MP, Rodriguez D, Nicolas C, Rodriguez PL, Nicolas G, Lorenzo O (2003). Negative regulation of abscisic acid signaling by the Fagus sylvatica FsPP2C1 plays a role in seed dormancy regulation and promotion of seed germination. Plant Physiol., 133: 135-144.
http://dx.doi.org/10.1104/pp.103.025569
PMid:12970481 PMCid:PMC196589
 
Hashimoto M, Negi J, Young J, Israelsson M, Schroeder JI, Iba K (2006). Arabidopsis HT1 kinase controls stomatal movements in response to CO2. Nat. Cell Biol., 8: 391-397.
http://dx.doi.org/10.1038/ncb1387
PMid:16518390
 
Hubbard KE, Nishimura N, Hitomi K, Getzoff ED, Schroeder JI (2010). Early abscisic acid signal transduction mechanisms: newly discovered components and newly emerging questions. Genes Dev., 24(16): 1695-1708.
http://dx.doi.org/10.1101/gad.1953910
PMid:20713515 PMCid:PMC2922499
 
Kerk D, Conley TR, Rodriguez FA, Tran HT, Nimick M, Muench DG, Moorhead GB (2006). A chloroplast-localized dual-specificity protein phosphatase in Arabidopsis contains a phylogenetically dispersed and ancient carbohydrate-binding domain, which binds the polysaccharide starch. Plant J., 46: 400-413.
http://dx.doi.org/10.1111/j.1365-313X.2006.02704.x
PMid:16623901
 
Kuhn JM, Boisson-Dernier A, Dizon MB, Maktabi MH, Schroeder JI (2006). The protein phosphatase AtPP2CA negatively regulates abscisic acid signal transduction in Arabidopsis, and effects of abh1 on AtPP2CA Mrna. Plant Physiol., 140: 127-139.
http://dx.doi.org/10.1104/pp.105.070318
PMid:16361522 PMCid:PMC1326037
 
Lee JS, Ellis BE (2007). Arabidopsis MAPK phosphatase 2 (MKP2) positively regulates oxidative stress tolerance and inactivates the MPK3 and MPK6 MAPKs. J. Biol. Chem., 282: 25020–25029.
http://dx.doi.org/10.1074/jbc.M701888200
PMid:17586809
 
Lee SC, Lan W, Buchanan BB, Luan S (2009). A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells. Proc. Natl. Acad. Sci. U S A., 106(50): 21419-21424.
http://dx.doi.org/10.1073/pnas.0910601106
PMid:19955427 PMCid:PMC2795491
 
Leonhardt N, Kwak JM, Robert N, Waner D, Leonhardt G, Schroeder JI (2004). Microarray expression analyses of Arabidopsis guard cells and isolation of a recessive abscisic acid hypersensitive protein phosphatase 2C mutant. Plant cell, 16: 596-615.
http://dx.doi.org/10.1105/tpc.019000
PMid:14973164 PMCid:PMC385275
 
Liang YK, Dubos C, Dodd IC, Holroyd GH, Hetherington AM, Campbell MM (2005). AtMYB61, an R2R3-MYB transcription factor controlling stomatal aperture in Arabidopsis thaliana. Curr. Biol., 15: 1201-1206.
http://dx.doi.org/10.1016/j.cub.2005.06.041
PMid:16005292
 
Ma Y, Szostkiewicz I, Korte A, Moes D, Yang Y, Christmann A, Grill E. (2009). Regulators of PP2C phosphatase activity function as abscisic acid sensors. Sciences, 324: 1064-1068.
 
Merlot S, Gosti F, Guerrier D, Vavasseur A, Giraudat J (2001). The ABI1 and ABI2 protein phosphatases 2C act in a negative feedback regulatory loop of the abscisic acid signalling pathway. Plant J., 25: 295-303.
http://dx.doi.org/10.1046/j.1365-313x.2001.00965.x
PMid:11208021
 
Pei ZM, Kuchitsu K, Ward JM, Schwarz M, Schroeder JI (1997). Differential abscisic acid regulation of guard cell slow anion channels in Arabidopsis wild-type and abi1 and abi2 mutants. Plant Cell, 9: 409-423.
http://dx.doi.org/10.2307/3870491
http://dx.doi.org/10.1105/tpc.9.3.409
PMid:9090884 PMCid:PMC156927
 
Reyes D, Rodriguez D, Gonzalez-Garcia MP, Lorenzo O, Nicolas G, Garcia-Martinez JL, Nicolas C (2006). Overexpression of a protein phosphatase 2C from beech seeds in Arabidopsis shows phenotypes related to abscisic acid responses and gibberellin biosynthesis. Plant Physiol., 141: 1414-1424.
http://dx.doi.org/10.1104/pp.106.084681
PMid:16815952 PMCid:PMC1533961
 
Rubio S, Rodrigues A, Saez A, Dizon MB, Galle A, Kim TH, Santiago J, Flexas J, Schroeder JI, Rodriguez PL (2009). Triple loss of function of protein phosphatases type 2C leads to partial constitutive response to endogenous abscisic acid. Plant Physiol., 150: 1345-1355.
http://dx.doi.org/10.1104/pp.109.137174
PMid:19458118 PMCid:PMC2705020
 
Saez A, Apostolova N, Gonzalez-Guzman M, Gonzalez-Garcia MP, Nicolas C, Lorenzo O, Rodriguez PL (2004). Gain-of-function and loss of-function phenotypes of the protein phosphatase 2C HAB1reveal its role as a negative regulator of abscisic acid signaling. Plant J., 37: 354-369.
http://dx.doi.org/10.1046/j.1365-313X.2003.01966.x
PMid:14731256
 
Saez A, Robert N, Maktabi MH, Schroeder JI, Serrano R, Rodriguez PL (2006). Enhancement of abscisic acid sensitivity and reduction of water consumption in Arabidopsis by combined inactivation of the protein phosphatases type 2C ABI1 and HAB1. Plant Physiol., 141: 1389-1399.
http://dx.doi.org/10.1104/pp.106.081018
PMid:16798945 PMCid:PMC1533955
 
Sheen J (1998). Mutational analysis of protein phosphatase 2C involved in abscisic acid signal transduction in higher plants. Proc. Natl. Acad. Sci. U.S.A., 95: 975-980.
http://dx.doi.org/10.1073/pnas.95.3.975
PMid:9448270 PMCid:PMC18643
 
Shin H, Shin HS, Guo Z, Blancaflor EB, Masson PH, Chen R (2005). Complex regulation of Arabidopsis AGR1/PIN2-mediated root gravitropic response and basipetal auxin transport by cantharidin-sensitive protein phosphatases. Plant J., 42: 188-200.
http://dx.doi.org/10.1111/j.1365-313X.2005.02369.x
PMid:15807782
 
Sokolov LN, Dominguez-Solis JR, Allary AL, Buchanan BB, Luan S (2006). A redox-regulated chloroplast protein phosphatase binds to starch diurnally and functions in its accumulation. Proc. Natl. Acad. Sci. U.S.A., 103: 9732-9737.
http://dx.doi.org/10.1073/pnas.0603329103
PMid:16772378 PMCid:PMC1480475
 
Tahtiharju S, Palva T (2001). Antisense inhibition of protein phosphatase 2C accelerates cold acclimation in Arabidopsis thaliana. Plant J., 26: 461-470.
http://dx.doi.org/10.1046/j.1365-313X.2001.2641048.x
http://dx.doi.org/10.1046/j.1365-313X.2001.01048.x
PMid:11439132
 
Ulm R, Ichimura K, Mizoguchi T, Peck SC, Zhu T, Wang X, Shinozaki K, Paszkowski J (2002). Distinct regulation of salinity and genotoxic stress responses by Arabidopsis MAP kinase phosphatase 1. EMBO J., 21: 6483-6493.
http://dx.doi.org/10.1093/emboj/cdf646
PMid:12456655 PMCid:PMC136950
 
Umezawa T, Sugiyama N, Mizoguchi M, Hayashi S, Myouga F, Yamaguchi-Shinozaki K, Ishihama Y, Hirayama T, Shinozaki K (2009). Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A., 106: 17588-17593.
http://dx.doi.org/10.1073/pnas.0907095106
PMid:19805022 PMCid:PMC2754379
 
Vlad F, Rubio S, Rodrigues A, Sirichandra C, Belin C, Robert N, Leung J, Rodriguez PL, Laurière C, Merlot S (2009). Protein phosphatases 2C regulate the activation of the Snf1-related kinase OST1 by abscisic acid in Arabidopsis. Plant Cell, 21: 3170-3184.
http://dx.doi.org/10.1105/tpc.109.069179
PMid:19855047 PMCid:PMC2782292
 
Xie X, Wang Y, Williamson L, Holroyd GH, Tagliavia C, Murchie E, Theobald J, Knight MR, Davies WJ, Leyser HM, Hetherington AM (2006). The identification of genes involved in the stomatal response to reduced atmospheric relative humidity. Curr. Biol., 16: 882–887.
http://dx.doi.org/10.1016/j.cub.2006.03.028
PMid:16682349
 
Xue T, Wang D, Zhang S, Ehlting J, Ni F, Jakab S, Zheng C, Zhong Y (2008). Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis. BMC Genomics, 9: 550.
http://dx.doi.org/10.1186/1471-2164-9-550
PMid:19021904 PMCid:PMC2612031
 
Yoshida T, Nishimura N, Kitahata N, Kuromori T, Ito T, Asami T, Shinozaki K, Hirayama T (2006). ABA-hypersensitive germination3 encodes a protein phosphatase 2C (AtPP2CA) that strongly regulates abscisic acid signaling during germination among Arabidopsis protein phosphatase 2Cs. Plant Physiol., 140: 115-126
http://dx.doi.org/10.1104/pp.105.070128
PMid:16339800 PMCid:PMC1326036

 


APA (2011). Overexpression of Arabidopsis PP2CA2 gene shows phenotypes related to abscisic acid responses. Journal of Cell Biology and Genetics, 2(1), 1 - 7.
Chicago Guo Xinhong, Cui Yi, Wang Jie, Zhao Qiong, Hu Shuai, Tang Dongying and Liu Xuanming. "Overexpression of Arabidopsis PP2CA2 gene shows phenotypes related to abscisic acid responses." Journal of Cell Biology and Genetics 2, no. 1 (2011): 1 - 7.
MLA Guo Xinhong, et al. "Overexpression of Arabidopsis PP2CA2 gene shows phenotypes related to abscisic acid responses." Journal of Cell Biology and Genetics 2.1 (2011): 1 - 7.
   
DOI
URL http://academicjournals.org/journal/JCBG/article-abstract/3F6D6CD909

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