Journal of
Cell Biology and Genetics

  • Abbreviation: J. Cell Biol. Genet.
  • Language: English
  • ISSN: 2141-6516
  • DOI: 10.5897/JCBG
  • Start Year: 2010
  • Published Articles: 13

Full Length Research Paper

Constitutive activation of AMP-activated protein kinase (AMPK) propel mitochondrial biogenesis

Paul B. Bokko*
  • Paul B. Bokko*
  • School of Life Sciences, La Trobe University, Bundoora, VIC 3086, Australia. Department of Veterinary Surgery and Theriogenology, University of Maiduguri, PMB 1069, Nigeria.
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Afsar U. Ahmed
  • Afsar U. Ahmed
  • School of Life Sciences, La Trobe University, Bundoora, VIC 3086, Australia. Monash Medical Research Institute, Monash University, Clayton, VIC 3800 Australia.
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Paul R. Fisher
  • Paul R. Fisher
  • School of Life Sciences, La Trobe University, Bundoora, VIC 3086, Australia.
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  •  Received: 03 August 2014
  •  Accepted: 19 September 2014
  •  Published: 30 September 2014

Abstract

Mitochondrial biogenesis is primarily a critical adaptation aimed to balance an increased workload in an attempt to maintain appropriate body perfusion. Until recently, the signaling mechanisms responsible for this response are poorly understood. To examine the role of AMP-activated protein kinase (AMPK), an evolutionarily conserved fuel sensor, in mitochondrial biogenesis, we used constitutively active and antisense inhibition genetic tools in Dictyostelium discoideum. Constitutive activation of AMPK culminated in mitochondrial proliferation and elevated ATP generation; this became marked with higher plasmid copies. Antisense inhibition of AMPK yielded non–significant decrease in the mitochondrial content at low levels. However, the more severe the antisense inhibition, the more significant the diminution of AMPK function, resulting in the more apparent decrease in the Advanced Technology Program (ATP) and mitotracker fluorescence. This finding provides direct genetic evidence that AMPK plays a significant role in ameliorating the effects of cellular energy deficit through mitochondrial proliferation. Thus, the constitutive activation of AMPK initiates signalling to downstream targets. The result perturbation of these pathways would culminate in the mitochondrial biogenesis. Taken together, these findings show the constitutive activation of AMPK propels in vivo mitochondrial biogenesis and ATP generation in D. discoideum as in other organisms.

 

Key words: Antisense, constitutive activation, Dictyostelium discoideum, mitochondrial mass, plasmid copies.