Journal of Cell Biology and Genetics
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Article Number - 797C9B1839


Vol.1(1), pp. 12 - 19 , September 2010

ISSN: 2141-6516



Full Length Research Paper

Proliferation of Juglans regia L. by in vitro embryo culture



S. Toosi
  • S. Toosi
  • Marand Islamic Azad University, Graduate studies, Mirand, Iran.
  • Google Scholar
K. Dilmagani
  • K. Dilmagani
  • Islamic Azad University of Marand, Marand, Iran.
  • Google Scholar







 Accepted: 23 June 2010  Published: 30 September 2010

Copyright © 2010 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


One of the new and important ways of plant propagation is micropropagation or tissue culture technique. Using this technique is preferred due to its high propagation of plants in short periods. This research tried to find the optimal culture media for the in vitro embryo growth and proliferation of Persian walnut (Juglans regia L.). In the first stage of this study, the effects of MS, DKW, NGE, WPM culture media were studied and compared, all without growth regulators. In second stage, five concentrations of BAP (0, 0.1, 0.5, 1, 2 mgl-1) and five concentrations of IBA (0, 0.1, 0.2, 0.4, 0.5 mgl-1) were applied. Experiments were carried out under conditions of 25°C, light/dark period of 16 and 8 h and a light intensity of 3000 lux. Results showed that the best growth percentage was obtained in NGE (61.1%) and the optimal treatment was (0.5 mg/l BAP, 0 mg/l IBA).

 

Key words: Proliferation, embryo culture, in vitro, walnut, Juglans.

 

Amiri ME (2004) Effect of mineral concentration on tissue cultured walnut (Juglans nigra var. zeiabadi) growth. International Society for Horticultural Science, p. 705.
 
Bonga JM, Aderkas PV (1992). In vitro culture of trees. 2(4): 35-38
 
Bosela MJ, Michler CH (2008a) Media effects on black walnut (Juglans nigra L.) shootculture growth in vitro: evaluation of multiplenutrient formulations and cytokinin types. In vitro Cell. Dev. Biol.-Plant 44:316–329.
http://dx.doi.org/10.1007/s11627-008-9114-5
 
Bosela MJ, Michler CH (2008b) Media effects on black walnut (Juglans regia L.) shoot culture growth in vitro: evaluation of multiple nutrient formulation and cytokinin types. In vitro cell Dev. Biol. Plant., 44:116-121
http://dx.doi.org/10.1007/s11627-008-9114-5
 
Bourrain L (2000). In vitro walnut micropropagation (Juglans nigra L.) application. CTIFL, pp. 30-127.
 
Cossio F, Minotta G (1983). Prove preliminary di coltura in vitro di embrioni isolati di noce (Juglans regia L.) e confronto tra differenti combinaciondi sali minerali. Riv. Ortoflorofrutt. It., 67: 287-298.
 
Driver, JA, Kuniyuki AH (1984) In vitro propagation of paradox walnut rootstock, Hotr. Sci., 19: 507-709
 
Jay-Allemand CH (1982) Culture in vitro du noger (Juglans sp.) In: Etude experimentale sur l'ensemencement d'embryons isoles et de bourgeons,Mem D, Univ Sci Tchn Languedoc, Mompelier, Fr.
 
Kaur R, Kumar K, Sharma DR, Sharma SD, Sharma N (2006). In vitro germination of walnut (Juglans regia L.) embryos, Scientia Horticulturae, 109: 385–388.
http://dx.doi.org/10.1016/j.scienta.2006.05.012
 
Leslie C, Mcgranahan G (1992). Micropropagation of Persian walnut (Juglans regia L.). Biotechnology in agriculture and forestry, p. 136-150.
 
McGranahan G, Leslie CA, Driver JA (1988). In vitro propagation of mature walnut cultivars. HortSci. 23: 220.
 
Revilla MA, Majada J, Rodriguez R (1989). Walnut (Juglans regia L.) micropropagation Ann. Sci. For. (Paris) 46: 149-151.
 
Rodriguez R (1982). Stimulation of multiple shoot-bud formation in walnuts seeds. HortScience 17(4): 592.
 
Rodriguez R, Lopez C, Diaz-Sala C, Berros B (1993). Simultaneous shoot bud development on walnut tissues of different ages: macro morphological and histological analyses. Acta Hort., 311: 141-152.
 
Rodriguez R, Revill A, Albuerne M, Perez C (1989). Walnut (Juglansspp.) In: Bajaj, Y. P. S.(Ed.), Biotechnology in Agriculture and Forestry, TreesII, 5: 99–125.
 
Saadat YA, Hennerty MJ (2002). Factors affecting shoot multiplication of Persian walnut (Juglans regia L.). Scientia Horticulturae 95: 257-260.
http://dx.doi.org/10.1016/S0304-4238(02)00003-1
 
Sanchez-Zamora MA, Cos - terrer J, Frutos-Tomas D, Garcia-Lopez, R. (2006). Embryo germination and proliferation in vitro of Juglans regia L., Scientia Horticulturae. 108:317- 321.
http://dx.doi.org/10.1016/j.scienta.2006.01.041
 
Scaltsoyiannes A, Tsoulpha P, Panetsos KP, Moulalis D (1997). Effect of Genotype on Micropropagation of Walnut Trees (Juglans regia). Silvae Genetica: 46: 6.
 
Tessitura T, Tsukuda K, Kawase K (2002). Micropropagation of shinano walnut (Juglans regia L.). J. Japan. Soc. Hort. Sci., 17:661-666.

 


APA (2010). Proliferation of Juglans regia L. by in vitro embryo culture. Journal of Cell Biology and Genetics, 1(1), 12 - 19.
Chicago S. Toosi and K. Dilmagani. "Proliferation of Juglans regia L. by in vitro embryo culture." Journal of Cell Biology and Genetics 1, no. 1 (2010): 12 - 19.
MLA S. Toosi and K. Dilmagani. "Proliferation of Juglans regia L. by in vitro embryo culture." Journal of Cell Biology and Genetics 1.1 (2010): 12 - 19.
   
DOI
URL http://academicjournals.org/journal/JCBG/article-abstract/797C9B1839

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