Journal of Clinical Immunology and Immunopathology Research
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Article Number - 297111760090


Vol.7(1), pp. 1-6 , July 2016
DOI: 10.5897/JCIIR2016.0076
ISSN: 1996-0816



Full Length Research Paper

Interferon-gamma (IFN-γ) and Interleukin-2 (IL2) as immunological markers in pulmonary tuberculosis



Amr Mohamed El-Sabbagh
  • Amr Mohamed El-Sabbagh
  • Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Egypt.
  • Google Scholar
Samah Sabry El-Kazzaz
  • Samah Sabry El-Kazzaz
  • Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Egypt.
  • Google Scholar
Ghada El-Saeed Mashaly
  • Ghada El-Saeed Mashaly
  • Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Egypt.
  • Google Scholar
Wael Alkhiary
  • Wael Alkhiary
  • Department of Clinical Pathology and Immunology, Faculty of Medicine, Mansoura University, Egypt.
  • Google Scholar
Tarek Fouad Sheta
  • Tarek Fouad Sheta
  • Department of Internal Medicine, Faculty of Medicine, Mansoura University, Egypt.
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 Received: 18 May 2016  Accepted: 27 June 2016  Published: 31 July 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Early diagnosis and treatment are important in prevention of tuberculosis (TB) infection. World commonly diagnose pulmonary TB using ZN stain, culture and TST. These tests are either low sensitive or required a long time. Recently intracellular interferon gamma flow cytometry assay has been available for diagnosis of pulmonary TB. We assess this diagnostic method in the diagnosis of pulmonary TB with special concern on its role and interleukin-2 (IL2) as tools to observe the effectiveness of anti-tuberculosis therapy. In our study we aimed to evaluate the diagnostic potential of flow cytometry assay for diagnosis of active pulmonary TB, assess the levels of intracellular Interferon-gamma (IFN-γ) and IL2 in patients with pulmonary TB as tools to observe the effectiveness of anti-tuberculosis therapy and correlate between the levels of IFN-γ and IL2 in the patients with the clinical and radiological findings. In our study intracellular interferon gamma flow cytometry assay and IL2 release were evaluated for pulmonary TB patients, LTBI persons and healthy control persons. Flow cytometry were done twice for the pulmonary TB patients, first at the start of treatment and second after 3 months of treatment. We confirmed that the intracellular interferon gamma flow cytometry assay after M. tuberculosis-specific stimulation is sufficient to recognize active TB. IL-2 were more frequently observed in latent TB infected individuals in contrast to active TB patients, and declined with advanced stage of TB. Intracellular interferon gamma flow cytometry assay could function as a powerful immunodiagnostic test to explore pulmonary TB and to predict the efficacy of antituberculosis treatment after 3 months of treatment in cases of pulmonary TB while, IL2 cannot be used to monitor the efficacy of antituberculosis treatment but it declined with advanced stage (stage III) of pulmonary TB in compare to stages I and II.

Key words: Intracellular Interferon-gamma (IFN-γ), flow cytometry, pulmonary tuberculosis (TB), interleukin-2 (IL2).

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APA El-Sabbagh, A. M., El-Kazzaz, S. S., Mashaly, G. E., Alkhiary, W., & Sheta, T. F. (2016). Interferon-gamma (IFN-γ) and Interleukin-2 (IL2) as immunological markers in pulmonary tuberculosis. Journal of Clinical Immunology and Immunopathology Research, 7(1), 1-6.
Chicago Amr Mohamed El-Sabbagh, Samah Sabry El-Kazzaz, Ghada El-Saeed Mashaly, Wael Alkhiary and Tarek Fouad Sheta. "Interferon-gamma (IFN-γ) and Interleukin-2 (IL2) as immunological markers in pulmonary tuberculosis." Journal of Clinical Immunology and Immunopathology Research 7, no. 1 (2016): 1-6.
MLA Amr Mohamed El-Sabbagh, et al. "Interferon-gamma (IFN-γ) and Interleukin-2 (IL2) as immunological markers in pulmonary tuberculosis." Journal of Clinical Immunology and Immunopathology Research 7.1 (2016): 1-6.
   
DOI 10.5897/JCIIR2016.0076
URL http://academicjournals.org/journal/JCIIR/article-abstract/297111760090

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