Journal of Clinical Immunology and Immunopathology Research
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Article Number - 573DB3D5877


Vol.5(1), pp. 1-8 , April 2013
DOI: 10.5897/JCIIR2012.0058
ISSN: 1996-0816



Full Length Research Paper

Cytokine levels associated with experimental malaria pathology during Plasmodium berghei ANKA infection in a mouse model



Mugweru Julius
  • Mugweru Julius
  • Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000-00200, Nairobi, Kenya.Kenya Medical Research Institute, Kenya.
  • Google Scholar
Waihenya Rebecca
  • Waihenya Rebecca
  • Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000-00200, Nairobi, Kenya.
  • Google Scholar
Kimani Francis
  • Kimani Francis
  • Kenya Medical Research Institute, Kenya.
  • Google Scholar
Ng’ang’a Zipporah
  • Ng’ang’a Zipporah
  • Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000-00200, Nairobi, Kenya.
  • Google Scholar
Matiru Viviene
  • Matiru Viviene
  • Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000-00200, Nairobi, Kenya.
  • Google Scholar
Francis W. Muregi
  • Francis W. Muregi
  • Kenya Medical Research Institute, Kenya.Mount Kenya University, Kenya.
  • Google Scholar







 Accepted: 05 February 2013  Published: 30 April 2013

Copyright © 2013 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Successful recovery from malaria involves striking a balance between counteracting cytokines. The cytokine imbalance contributes to pathological features, but their exact levels have not been elucidated. This study aimed at investigating the role played by circulating cytokines in pathophysiology of cerebral malaria using experimental cerebral malaria (ECM) model by profiling four serum cytokines using cytometric bead array. 72 BALB/c mice were intraperitoneally inoculated with 0.2 ml 1×104 parasitized red blood cells at day 0 and randomized into six groups (six mice/group). The mice were sacrificed at day 4, 6, 8, 11 and 20 post-infection. Significantly higher systemic levels (P<0.05) of interferon-gamma (IFN-γ) were observed between day 8 and 20 post inoculation (p.i), while tumor necrosis factor-alpha (TNF-α) levels were significant at days 4, 8 11, 14 and 20, respectively in BALB/c mice that survived until day 20 post-infection with a higher parasitemia (up to 52.6%±0.8). Significant concentrations (P<0.05) of interleukin-4 (IL-4) were observed between day 4 and 8. IL-5 levels had significant differences at days 11 and 20 p.i. T-cell pathology was revealed by fragmentation of whole genomic DNA during the infection which coincided with elevated IFN-γ and TNF-α responses further accelerating the severity of cerebral malaria (CM). This study has demonstrated the correlation between T-lymphocyte pathology and elevated levels of T-helper 1 (Th1) cytokines concentrations in the brain and spleen.

 

Key words: Experimental malaria, Plasmodium berghei, pathology, cytokine levels, fragmentation.

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APA Mugweru, J., Waihenya, R., Kimani, F., Ng’ang’a, Z., Matiru, V., & Muregi, F. W. (2013). Cytokine levels associated with experimental malaria pathology during Plasmodium berghei ANKA infection in a mouse model. Journal of Clinical Immunology and Immunopathology Research, 5(1), 1-8.
Chicago Mugweru Julius, Waihenya Rebecca, Kimani Francis, Ng’ang’a Zipporah, Matiru Viviene and Francis W. Muregi,. "Cytokine levels associated with experimental malaria pathology during Plasmodium berghei ANKA infection in a mouse model." Journal of Clinical Immunology and Immunopathology Research 5, no. 1 (2013): 1-8.
MLA Mugweru Julius, et al. "Cytokine levels associated with experimental malaria pathology during Plasmodium berghei ANKA infection in a mouse model." Journal of Clinical Immunology and Immunopathology Research 5.1 (2013): 1-8.
   
DOI 10.5897/JCIIR2012.0058
URL http://academicjournals.org/journal/JCIIR/article-abstract/573DB3D5877

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