Journal of Cancer Research and Experimental Oncology
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Article Number - 3FFE5EC58494


Vol.8(1), pp. 1-14 , April 2016
DOI: 10.5897/JCREO2016.0127
ISSN: 2141-2243



Review

Diallyl disulfide protects against rectal cancer in vivo model of male rabbits: II-Analysis of histological and cytogenetic variations



Tito N. Habib*
  • Tito N. Habib*
  • Molecular Genetics Laboratory, Zoology Department, Faculty of Science, Sohag University, Sohag, Egypt.
  • Google Scholar
Mohammed O. Altonsy
  • Mohammed O. Altonsy
  • Molecular Genetics Laboratory, Zoology Department, Faculty of Science, Sohag University, Sohag, Egypt.
  • Google Scholar
Soheir A. Abd El-Raheem
  • Soheir A. Abd El-Raheem
  • Animal Physiology Laboratory, Zoology Department, Faculty of Science, Sohag University, Sohag, Egypt.
  • Google Scholar
Yassmin R. Bakeer
  • Yassmin R. Bakeer
  • Animal Physiology Laboratory, Zoology Department, Faculty of Science, Sohag University, Sohag, Egypt.
  • Google Scholar







 Received: 10 February 2016  Accepted: 21 April 2016  Published: 30 April 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The present work was conducted to perform a short-term comparative analysis and evaluate the anti-neoplastic effects of diallyl disulfide on rectal carcinogenicity via histopathological changes, chromosomal aberrations, and mitotic index induced by 1,2-dimethylhydrazine on male rabbits (Orectolagus cuniculus). The histological changes that can be seen microscopically showed that 1,2-dimethylhydrazine at the suggested dose (20 mg/kg) produced significant alterations in rectal mucosa of 1,2-dimethylhydrazine group. The presence of dysplasia was regarded as an early histopathological changes in the precursor lesions of rectal cancer. Three varieties of intrachromosomal instability were detected, deletions (1p12, 15q23, 21q14), duplications (5q14; 13q23, 14q21) and ring (X) chromosome with a highly significant increase (P<0.05) in comparison with control. Such aberrations were markedly inclined in 1,2- dimethylhydrazine group after treatment by diallyl disulfide and the pretreated group  that received diallyl disulfide prior to 1,2-dimethylhydrazine injection with a significant decrease (P<0.01). Mitotic index ranged from 46, 22, 17, and 18% to 20% in 1,2-dimethylhydrazine, 1,2-dimethylhydrazine +diallyl disulfide, diallyl disulfide, control, and pretreated diallyl disulfide +1,2-dimethylhydrazine groups, respectively. Examination of 1,2-dimethylhydrazine group showed that it caused neoplastic changes with cytogenetic abnormality identified by hematoxylin and eosin staining and G-banding analysis, respectively. Such changes were similar to those seen in human sporadic colorectal carcinogenesis.

Key words: Rectal cancer, diallyl disulfide, dimethyl hydrazine, chromosomal aberrations, intrachromosomal instability, mitotic index.

Abbreviation:

CRC, Colorectal cancer ; RCC, rectal cancer; BP, cytoplasmic basophilia; Del, deletion; Dup, duplication; RC, rectal crypt; Rch, ring chromosome; H&E, hematoxylin and eosin; CAs, chromosomal aberrations; ICIN, intrachromosomal instability; CIN, chromosomal instability.

 

 


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APA Habib, T. N., Altonsy, M. O., Abd El-Raheem, S. A., & Bakeer, Y. R. (2016). Diallyl disulfide protects against rectal cancer in vivo model of male rabbits: II-Analysis of histological and cytogenetic variations. Journal of Cancer Research and Experimental Oncology, 8(1), 1-14.
Chicago Tito N. Habib, Mohammed O. Altonsy, Soheir A. Abd El-Raheem and Yassmin R. Bakeer. "Diallyl disulfide protects against rectal cancer in vivo model of male rabbits: II-Analysis of histological and cytogenetic variations." Journal of Cancer Research and Experimental Oncology 8, no. 1 (2016): 1-14.
MLA Tito N. Habib, et al. "Diallyl disulfide protects against rectal cancer in vivo model of male rabbits: II-Analysis of histological and cytogenetic variations." Journal of Cancer Research and Experimental Oncology 8.1 (2016): 1-14.
   
DOI 10.5897/JCREO2016.0127
URL http://academicjournals.org/journal/JCREO/article-abstract/3FFE5EC58494

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