Journal of Developmental Biology and Tissue Engineering
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Article Number - B40B1AE40395


Vol.5(2), pp. 12-22 , May 2012
https://doi.org/10.5897/JDBTE11.025
ISSN: 2141-2251


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Full Length Research Paper

Round-bottomed Honeycomb Microwells: Embryoid body shape correlates with stem cell fate


Silin Sa1, Diep T. Nguyen3, Jonathan D. Pegan2, Michelle Khine3 and Kara E. McCloskey1,2* 1Graduate Group in 




1Biological Engineering and Small-scale Technologies, University of California, Merced, USA.

2School of Engineering, University of California, Merced, USA. 3Department of Biomedical Engineering, University of California, Irvine, USA.


Email: [email protected]






 Accepted: 26 November 2011  Published: 31 May 2012

Copyright © 2012 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The differentiation of embryonic stem cells (ESC) into tissue-specific cells utilizes either monolayer cultures or three-dimensional cell aggregates called embryoid bodies (EB). However, the generation of a large number of EB of controlled sizes can be challenging and labor intensive. Our laboratories have developed a simple, robust, ultra-rapid, and inexpensive design of Honeycomb Microwells for generation of EB. Here, we compare EB generated using (1) Honeycomb Microwells, (2) the commercially available AggreWell™400, and (3) the more traditional Hanging Drop method. We compared the efficiency, viability, quality, and control of EB sizes. Results indicate that the Honeycomb Microwell and AggreWell™400 efficiently generate small EB at approximately 500 cells per EB. However, the cone-bottomed AggreWell plate generates cone-shaped EB at 1000-2000 cells per EB. Moreover, the cone-shape correlates with a reduction in the formation of the primitive endoderm GATA-4+ cells (1% compared with 6-8% in spherical EB), but does not significantly affect mesoderm or ectoderm development. We conclude that the non-spherical EB shape correlates with a reduction in the development of primitive endoderm, and that use of these AggreWell plates should be avoided in deriving endoderm tissue products. Key words: Embryonic stem cells, embryoid bodies, Microwell, AggreWell, hanging drop, primitive endoderm, GATA-4.


APA (2012). Round-bottomed Honeycomb Microwells: Embryoid body shape correlates with stem cell fate. Journal of Developmental Biology and Tissue Engineering, 5(2), 12-22.
Chicago Silin Sa, Diep T. Nguyen, Jonathan D. Pegan, Michelle Khine and Kara E. McCloskey, Graduate Group in . "Round-bottomed Honeycomb Microwells: Embryoid body shape correlates with stem cell fate." Journal of Developmental Biology and Tissue Engineering 5, no. 2 (2012): 12-22.
MLA Silin Sa, et al. "Round-bottomed Honeycomb Microwells: Embryoid body shape correlates with stem cell fate." Journal of Developmental Biology and Tissue Engineering 5.2 (2012): 12-22.
   
DOI https://doi.org/10.5897/JDBTE11.025
URL http://academicjournals.org/journal/JDBTE/article-abstract/B40B1AE40395

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