Journal of Developmental Biology and Tissue Engineering
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Article Number - B82C1FA40378


Vol.5(1), pp. 7-12 , April 2013
https://doi.org/10.5897/JDBTE12.003
ISSN: 2141-2251


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Full Length Research Paper

3-D osteoblast culture for biomaterials testing


Jörg Neunzehn*, Sascha Heinemann and Hans Peter Wiesmann1




Max Bergmann Center of Biomaterials and Institute of Material Science, Technische Universität Dresden, Budapester Str. 27, D-01069 Dresden, Germany.              


Email: [email protected]






 Accepted: 19 September 2012  Published: 30 April 2013

Copyright © 2013 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Micromass cell cultures, osteomicrospheres, were formed by aggregation of primary osteoblasts. Cell differentiation during sphere formation and the integrity of the osteomicrospheres was evaluated by analyzing the immunohistochemical expression of osteonectin, osteocalcin, and collagen type I. Transmission electron microscopy facilitated the proof of the tissue-like microstructure inside the osteomicrospheres and the arrangement of collagen fibers similar to early stages of natural bone formation. The in vivo situation of osteomicrospheres transferred to host tissue was simulated by embedding mature osteomicrospheres in a fibrin matrix. Additionally, cell spreading out of the compartments was investigated by electron microscopy of microspheres cultivated on the surface of three-dimensional fiber-like scaffolds. Concluding, the properties of the osteomicrospheres represented in this work demonstrate the potential as a tissue-specific in vitro test method to replace early small animal tests in the future.

Key words: Biomaterials testing, micromass culture, osteoblast, osteomicrospheres, ultrastructure.

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APA (2013). 3-D osteoblast culture for biomaterials testing. Journal of Developmental Biology and Tissue Engineering, 5(1), 7-12.
Chicago Jörg Neunzehn, Sascha Heinemann and Hans Peter Wiesmann. "3-D osteoblast culture for biomaterials testing." Journal of Developmental Biology and Tissue Engineering 5, no. 1 (2013): 7-12.
MLA Jörg Neunzehn, Sascha Heinemann and Hans Peter Wiesmann. "3-D osteoblast culture for biomaterials testing." Journal of Developmental Biology and Tissue Engineering 5.1 (2013): 7-12.
   
DOI https://doi.org/10.5897/JDBTE12.003
URL http://academicjournals.org/journal/JDBTE/article-abstract/B82C1FA40378

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