Journal of Developmental Biology and Tissue Engineering
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Article Number - B8A57C010632

Vol.5(2), pp. 18-25 , May 2013
ISSN: 2141-2251

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Full Length Research Paper

Markers are shared between adipogenic and osteogenic differentiated mesenchymal stem cells

Melanie Köllmer1, Jason S. Buhrman1, Yu Zhang1 and Richard A. Gemeinhart1,2,3*

1Department of Biopharmaceutical Sciences, University of Illinois, Chicago, IL 60612-7231, USA.2Department of Bioengineering, University of Illinois, Chicago, IL 60607-7052, USA.

3Department of Ophthalmology and Visual Sciences, University of Illinois, Chicago, IL 60612-4319, USA.

Email: [email protected]

 Accepted: 15 May 2013  Published: 31 May 2013

Copyright © 2013 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0

The stem cell differentiation paradigm is based on the progression of cells through generations of daughter cells that eventually become restricted and committed to one lineage resulting in fully differentiated cells. Herein, we report on the differentiation of adult human mesenchymal stem cells (hMSCs) towards adipogenic and osteogenic lineages using established protocols. Lineage specific genes were evaluated by quantitative real-time PCR relative to two reference genes. The expression of osteoblast-associated genes (alkaline phosphatase, osteopontin, and osteocalcin) was detected in hMSCs that underwent adipogenesis. The expression of adipocyte marker genes (adiponectin, fatty acid binding protein P4, and leptin) increased in a time-dependent manner during adipogenic induction. Adiponectin and leptin were also detected in osteoblast-induced cells. Lipid vacuoles that represent the adipocyte phenotype were only present in the adipogenic induction group. Conforming to the heterogeneous nature of hMSCs and the known plasticity between osteogenic and adipogenic lineages, these data indicate a marker overlap between MSC-derived adipocytes and osteoblasts. We propose a careful consideration of experimental conditions such as investigated time points, selected housekeeping genes and the evidence indicating lack of differentiation into other lineageswhen evaluating hMSC differentiation.


Key words: Mesenchymal stem cell, differentiation markers, cell plasticity, differentiation.

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APA (2013). Markers are shared between adipogenic and osteogenic differentiated mesenchymal stem cells. Journal of Developmental Biology and Tissue Engineering, 5(2), 18-25.
Chicago Melanie Köllmer, Jason S. Buhrman, Yu Zhang and Richard A. Gemeinhart,. "Markers are shared between adipogenic and osteogenic differentiated mesenchymal stem cells." Journal of Developmental Biology and Tissue Engineering 5, no. 2 (2013): 18-25.
MLA Melanie Kouml;llmer, et al. "Markers are shared between adipogenic and osteogenic differentiated mesenchymal stem cells." Journal of Developmental Biology and Tissue Engineering 5.2 (2013): 18-25.

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