Journal of Dentistry and Oral Hygiene
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Article Number - 1F1AF5242841


Vol.6(1), pp. 10-21 , January 2014
DOI: 10.5897/JDOH2013.0105
ISSN: 2141-2472



Full Length Research Paper

In vitro assessments of white-spot lesions treated with NaF plus tricalcium phosphate (TCP) toothpastes using synchrotron radiation micro computed tomography (SR micro-CT)



Makoto Asaizumi
  • Makoto Asaizumi
  • Dr. Makoto Asaizumi Orthodontic Practice, Ban Bldg., 3F., 1-8 Chiyodacho, Mobara, Chiba 297-0023, Japan.
  • Google Scholar
Kentaro Uesugi
  • Kentaro Uesugi
  • SPring-8/JASRI, 1-1-1 Kouto, Sayocho, Sayogun, Hyogo 679-5198, Japan.
  • Google Scholar
Masato Hoshino
  • Masato Hoshino
  • SPring-8/JASRI, 1-1-1 Kouto, Sayocho, Sayogun, Hyogo 679-5198, Japan.
  • Google Scholar
Tomoaki Kato
  • Tomoaki Kato
  • Dr. Tetsuya Kuga Dental Practice, 924 Kamiichiba, Mutsuzawamachi, Chouseigun, Chiba 299-4403, Japan.
  • Google Scholar
Allen C. Mackey
  • Allen C. Mackey
  • University of Michigan, 2122 Lurie Biomedical Engineering, 1101 Beal Avenue Ann Arbor, MI 48109-2110, USA.
  • Google Scholar
Robert L. Karlinsey
  • Robert L. Karlinsey
  • Indiana Nanotech, 14 East Washington Street, Suite 6A, Indianapolis, IN 46204, USA.
  • Google Scholar







 Accepted: 22 January 2014  Published: 31 January 2014

Copyright © 2014 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Synchrotron radiation micro-computed tomography (SR micro-CT), considered superior to standard polychromatic micro-CT techniques, was used to assess the densities of bovine enamel white-spot lesions (WSL) treated in a 10-day pH cycling model with either: (A) Clinpro Tooth Crème (0.21% NaF plus TCP), (B) Clinpro 5000 (1.1% NaF plus TCP) or (C) Tom’s of Maine (0% NaF) dentifrice. Each day consisted of four 2 min treatments, one 4 h acid challenge (pH=5.0), and immersion in artificial saliva (pH=7.0) between these events. After 10 days, WSL specimens were evaluated for lesion depth using confocal microscopy and lesion density using SR micro-CT with depths ranging from 2.76 to 113.16 μm, in 2.76 μm slice increments. Statistical analyses (Student’s t-test) were performed at the 95% confidence level. SR micro-CT analyses revealed the NaF plus TCP dentifrices improved WSL densities relative to the fluoride-free toothpaste, and is consistent with an earlier study utilizing polychromatic micro-CT. In contrast to previous findings, SR micro-CT analyses also revealed significant differences in WSL densities treated with the two NaF dentifrices at enamel depths of 13.80, 16.56, and 19.32 μm. These findings suggest SR micro-CT may be especially suited for detecting density differences in lesions sensitive to fluoride-driven remineralization processes.

Key words: Toothpaste, synchrotron radiation micro computed tomography (SR micro-CT), density, remineralization, fluoride, monochromatized X-ray beams, X-ray linear attenuation coefficient (X-ray LAC).

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APA Asaizumi, M., Uesugi, K., Hoshino, M., Kato, T., Mackey, A.C., & Karlinsey, R.L. (2014). In vitro assessments of white-spot lesions treated with NaF plus tricalcium phosphate (TCP) toothpastes using synchrotron radiation micro computed tomography (SR micro-CT). Journal of Dentistry and Oral Hygiene , 6(1), 10-21.
Chicago Makoto Asaizumi, Kentaro Uesugi, Masato Hoshino, Tomoaki Kato, Allen C. Mackey and Robert L. Karlinsey. "In vitro assessments of white-spot lesions treated with NaF plus tricalcium phosphate (TCP) toothpastes using synchrotron radiation micro computed tomography (SR micro-CT)." Journal of Dentistry and Oral Hygiene 6, no. 1 (2014): 10-21.
MLA Makoto Asaizumi, et al. "In vitro assessments of white-spot lesions treated with NaF plus tricalcium phosphate (TCP) toothpastes using synchrotron radiation micro computed tomography (SR micro-CT)." Journal of Dentistry and Oral Hygiene 6.1 (2014): 10-21.
   
DOI 10.5897/JDOH2013.0105
URL http://academicjournals.org/journal/JDOH/article-abstract/1F1AF5242841

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