Journal of Evolutionary Biology Research
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Article Number - 93CA06048098


Vol.6(3), pp. 9-12 , October 2014
DOI: 10.5897/JEBR2013.0054
ISSN: 2141-6583



Full Length Research Paper

Somatic embryogenesis in two Nigerian cassava cultivars (Sandpaper and TMS 60444)



Alfred O. Ubalua*
  • Alfred O. Ubalua*
  • Plant Tissue Culture Laboratory, Biotechnology Research and Development Center, National Root Crops Research Institute (NRCRI), Umudike, PMB 7006 Umuahia, Abia State, Nigeria.
  • Google Scholar
Ena Mbanaso
  • Ena Mbanaso
  • Iltab Laboratory, Donald Danforth Plant Science Center, 975 N, Warson Road, St. Louis, Mo 63132, USA.
  • Google Scholar







 Received: 23 April 2013  Accepted: 07 October 2014  Published: 30 October 2014

Copyright © 2014 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The embryogenic ability of two cassava cultivars (Sandpaper and TMS 60444) was investigated by culturing their young leaf lobes on an induction medium (DKW2 50P). The explants formed organized embryogenic structures regardless of the concentration of picloram in the medium. Organized embryogenic structures induction was enhanced by increasing the concentration of picloram in the induction medium for both cultivars. The optimum level of picloram at which maximum stable frequencies (53.1±17.6 and 51.5±14.6 for Sandpaper and TMS 60444 respectively) of organized embryogenic structures were obtained was 5 mg/l. Friable embryogenic callus were further produced in GD2 50P medium supplemented with 500 µM tyrosine. The produced friable embryogenic calluses are prime target tissues for genetic transformation and plantlet regeneration.

 

Key words: Cassava cultivars, explants, organized embryogenic structures, friable embryogenic callus, picloram, tyrosine.

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APA Ubalua, A. O., & Mbanaso, E. (2014). Somatic embryogenesis in two Nigerian cassava cultivars (Sandpaper and TMS 60444). Journal of Evolutionary Biology Research, 6(3), 9-12.
Chicago Alfred O. Ubalua, and Ena Mbanaso. "Somatic embryogenesis in two Nigerian cassava cultivars (Sandpaper and TMS 60444)." Journal of Evolutionary Biology Research 6, no. 3 (2014): 9-12.
MLA Alfred O. Ubalua, and Ena Mbanaso. "Somatic embryogenesis in two Nigerian cassava cultivars (Sandpaper and TMS 60444)." Journal of Evolutionary Biology Research 6.3 (2014): 9-12.
   
DOI 10.5897/JEBR2013.0054
URL http://academicjournals.org/journal/JEBR/article-abstract/93CA06048098

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