Journal of General and Molecular Virology
Subscribe to JGMV
Full Name*
Email Address*

Article Number - 1D5662345295


Vol.6(2), pp. 19-27 , June 2014
DOI: 10.5897/JGMV2014.0057
ISSN: 2141-6648



Full Length Research Paper

Molecular characterization of influenza A (H7N9) virus from the first imported H7N9 infection case in Malaysia



Jeyanthi S.*
  • Jeyanthi S.*
  • Virology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
  • Google Scholar
Tengku Rogayah T. A. R.
  • Tengku Rogayah T. A. R.
  • Virology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
  • Google Scholar
Thayan R.
  • Thayan R.
  • Virology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
  • Google Scholar
Az-UlHusna A.
  • Az-UlHusna A.
  • Virology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
  • Google Scholar
Aruna A.
  • Aruna A.
  • Virology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
  • Google Scholar
Khebir B. V.
  • Khebir B. V.
  • Disease Control Division, Ministry of Health Malaysia, Putrajaya. Malaysia
  • Google Scholar
Thevarajah B.
  • Thevarajah B.
  • Kumpulan Perubatan Johor Hospital,Kota Kinabalu, Sabah, Malaysia
  • Google Scholar
Maria S.
  • Maria S.
  • Sabah Health Department, Kota Kinabalu, Sabah Malaysia
  • Google Scholar
Zainah S.
  • Zainah S.
  • Virology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
  • Google Scholar







 Received: 24 April 2014  Accepted: 23 May 2014  Published: 30 June 2014

Copyright © 2014 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


H7N9 is an avian strain of the species Influenza virus that circulates among avian populations. Occasionally, some variants of this strain were known to infect humans. On March 30, 2013, a novel avian influenza A H7N9 virus that infects human beings was identified in China. In February 2014, the first case of H7N9 infection outside China was reported in Malaysia involving a Chinese tourist. This study was aimed to characterize the first case of H7N9 in Malaysia by means of molecular identification, sequencing of hemagglutinin (HA) and neuraminidase (NA) genes, and phylogenetic analysis. The patient was confirmed positive for H7N9 virus by real-time RT-PCR (rRT-PCR). Subsequently, the samples were sequenced and mutation analysis identified R65K, E122K, L186I and N285D mutations in HA gene and M26I, R78K and V345I mutations in NA gene. We reported the emergence of a new mutation L186I, not found in the current database of any H7N9 sequences. Mutations associated with drug resistance were not found in this patient. Phylogenetic analysis revealed that the HA gene is closely related to the group of strains from Guangzhou, whereas NA gene is closely related to the group of strains from Guangdong. The present study provides crucial information on the first case of H7N9 outside China and the diversity of this strain from other reported H7N9 strains by molecular analysis.

Key words: H7N9, avian strain, China, molecular analysis, influenza virus.

Centre for Disease Control (CDC) (2014). H7N9 Case Detected in Malaysia. 12 February 2014.
 
Cheepsattayakorn A, Cheepsattayakorn R (2013). Novel Avian Flu A (H7N9): Clinical and Epidemiological Aspects, and Management. Virol Mycol. 2(121). doi:10.4172/2161-0517.1000121.
Crossref
 
Drosten C, Seilmaier M, Corman VM, Hartmann W, Scheible G, Sack S, et al. (2013). Clinical features and virological analysis of a case of Middle East respiratory syndrome coronavirus infection. Lancet Infect Dis. 13(9):745-751.
Crossref
 
Gao R, Cao B, Hu Y, Feng Z, Wang D, Hu W, Chen J et al. (2013). Human Infection with a Novel Avian-Origin Influenza A (H7N9) Virus. N Engl J Med. 368(20):1888-1897.
Crossref
 
Gubareva LV (2004). Molecular mechanisms of influenza virus resistance to neuraminidase inhibitors. Virus Res. 103: 199 -203.
Crossref
 
Hoffmann E, Stech J, Guan Y, Webster RG, Perez DR (2001). Universal primer set for the full-length amplification of all influenza A viruses. Arch Virol. 146(12):2275-89.
Crossref
 
Lam TT, Wang J, Shen Y, Zhou B, Duan L, Cheung CL, et al (2013). The genesis and source of the H7N9 influenza viruses causing human infections in China Nature. 502:241-246.
 
Li Q, Zhou L, Zhou M, Chen Z, Li F, Wu H, Xiang N, et al. (2014). Epidemiology of Human Infections with Avian Influenza A(H7N9) Virus in China. N Engl J Med. 370:520-532.
Crossref
 
Mok CK, Chen GW, Shih KC, Gong TN, Lin SJ, Horng JT (2013). Evolutionary conserved residues at an oligomerization of the influenza A virus neuraminidase are essential for viral survival. Virol. 447(1-2):32-44.
Crossref
 
Shuihua L, Yufang Z, Tao L, Yunwen H, Xinian L, Xiuhong X, Qingguo C, et al. (2013). Clinical Findings for Early Human Cases of Influenza A (H7N9) Virus Infection, Shanghai, China. Emerging Inf Dis. 19(7):1142-1146.
 
Taylor NR, von Itzstein M (1994). Molecular modeling studies on ligand binding to sialidase from influenza virus and the mechanism of catalysis. J Med Chem. 37(5):616-624.
Crossref
 
Whitley RJ, Boucher CA, Lina B, Nguyen-Van-Tam JS, Osterhaus A, Schutten M, Monto AS. 2013. Global assessment of resistance to neuraminidase inhibitors, 2008-2011: the influenza resistance information study (IRIS). Clin. Infect Dis. 56:1197-1205.
Crossref
 
WHO. Global Alert and Response: Human infection with avian influenza A(H7N9) virus-update (2013). Accessed: 3rd March 2014.
 
WPRO. Human Infection with Avian Influenza A(H7N9)- update (2014). Accessed: 16 May 2014.

 


APA Jeyanthi, S., Tengku Rogayah, T. A. R., Thayan, R., Az-UlHusna, A., Aruna, A., Khebir, B. V., Thevarajah, B., Maria, S., & Zainah, S. (2014). Molecular characterization of influenza A (H7N9) virus from the first imported H7N9 infection case in Malaysia. Journal of General and Molecular Virology, 6(2), 19-27.
Chicago Jeyanthi S., Tengku Rogayah T. A. R., Thayan R., Az-UlHusna A., Aruna A., Khebir B. V., Thevarajah B., Maria S. and Zainah S.. "Molecular characterization of influenza A (H7N9) virus from the first imported H7N9 infection case in Malaysia." Journal of General and Molecular Virology 6, no. 2 (2014): 19-27.
MLA Jeyanthi S., et al. "Molecular characterization of influenza A (H7N9) virus from the first imported H7N9 infection case in Malaysia." Journal of General and Molecular Virology 6.2 (2014): 19-27.
   
DOI 10.5897/JGMV2014.0057
URL http://academicjournals.org/journal/JGMV/article-abstract/1D5662345295

Subscription Form