Journal of Infectious Diseases and Immunity
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Article Number - 0E212933425


Vol.3(1), pp. 6-13 , January 2011

ISSN: 2141-2375



Full Length Research Paper

siRNA-mediated silencing of translationally controlled tumor protein induces apoptosis and inhibits cell growth


Zeng-Chun Ma, Qian Hong, Yu-Guang Wang, Hong-Ling Tan, Cheng-Rong Xiao, Qian-De Liang, Bei-bei Lu and Yue Gao*




Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing, 100850, P. R. China.


Email: gaoyue@yahoo.com, mazengchun2008@gmail.com






 Accepted: 19 December 2010  Published: 31 January 2011

Copyright © 2011 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Translationally controlled tumor protein (TCTP) is an evolutionally highly conserved protein which has been implicated in many cellular functions that are related to cell growth, death and tumorigenesis. We used a DNA vector-based RNA interference approach by expressing shRNA to knockdown TCTP in MCF7 cells for evaluating its antitumor effect and its molecular mechanism. TCTP shRNA treatment MCF7 cells suppressed cell growth and proliferation. In addition, cell cycle arrest and apoptotic cell death were observed from TCTP shRNA-treated MCF7 cells. Myeloid cell leukemia 1 (MCL-1) was identified to be related with TCTP in cell growth and apoptosis cell death. These results suggested that vector-based TCTP RNA interference could be an efficient molecular therapeutic method to kill MCF7 cells, and TCTP played pivotal roles in cell growth and apoptosis cell death.

 

Key words: TCTP, RNA interference, apoptosis, cell cycle.


APA (2011). siRNA-mediated silencing of translationally controlled tumor protein induces apoptosis and inhibits cell growth. Journal of Infectious Diseases and Immunity, 3(1), 6-13.
Chicago Zeng-Chun Ma, Qian Hong, Yu-Guang Wang, Hong-Ling Tan, Cheng-Rong Xiao, Qian-De Liang, Bei-bei Lu and Yue Gao. "siRNA-mediated silencing of translationally controlled tumor protein induces apoptosis and inhibits cell growth." Journal of Infectious Diseases and Immunity 3, no. 1 (2011): 6-13.
MLA Zeng-Chun Ma, et al. "siRNA-mediated silencing of translationally controlled tumor protein induces apoptosis and inhibits cell growth." Journal of Infectious Diseases and Immunity 3.1 (2011): 6-13.
   
DOI
URL http://academicjournals.org/journal/JIDI/article-abstract/0E212933425

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