Journal of
Infectious Diseases and Immunity

  • Abbreviation: J. Infect. Dis. Immun.
  • Language: English
  • ISSN: 2141-2375
  • DOI: 10.5897/JIDI
  • Start Year: 2009
  • Published Articles: 75

Full Length Research Paper

Analysis of Beta-lactamase production and Antibiotics resistance in Staphylococcus aureus strains

Torimiro N
  • Torimiro N
  • Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria.
  • Google Scholar
Moshood AA
  • Moshood AA
  • Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria.
  • Google Scholar
Eyiolawi SA
  • Eyiolawi SA
  • Department of Botany and Microbiology, University of Ibadan, Ibadan, Nigeria.
  • Google Scholar


  •  Published: 30 September 2013

Abstract

The antibiotic susceptibility profile and the association of β-lactamase production to antibiotic resistance in Staphylococcus aureus strains were analysed. The disk diffusion antibiotic susceptibility pattern was conducted on S. aureus strains cultured from clinical specimens using standard bacteriological methods. The β-lactamase production was assayed using the modified Perret’s iodometric assay and data were statistically analysed. Of the 107 S. aureus isolates identified, 77 (72%) of the isolates were multi-drug resistant and 75 (70.1%) produced β-lactamase.  β-lactamase production and resistance to  amoxicillin, amoxicillin/clavulanic acid (augmentin) and ceftriaxone resistance was significant (p-value< 0.05). The study suggests strict infection control measures and encouragement of prudent antibiotic use.

 

Key words: Staphylococcus aureus, Antibiotics, Beta-lactamase, Resistance