Journal of Microbiology and Antimicrobials
Subscribe to JMA
Full Name*
Email Address*

Article Number - 45083DB59795


Vol.8(5), pp. 28-33 , July 2016
DOI: 10.5897/JMA2016.0361
ISSN: 2141-2308



Full Length Research Paper

Phytochemical and antibacterial investigations of moringa (Moringa oleifera) leaf extract on selected bacterial pathogens



Abubakar Idris
  • Abubakar Idris
  • School of Applied and Natural Science, Microbiology Unit, Niger State Polytechnic, Zungeru, Niger State, Nigeria.
  • Google Scholar
Usman Abubakar
  • Usman Abubakar
  • School of Physical Science, Department of Statistics, Federal University of Technology, Minna, Niger State, Nigeria.
  • Google Scholar







 Received: 09 January 2016  Accepted: 11 May 2016  Published: 31 July 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


This study aimed at evaluating the health benefits of popular Moringa oleifera leaf. The aqueous and methanolic extracts of the leaf at two different concentrations (1:1 and 1:2) was used to determine the phytochemical screening and its antibacterial activity. Escherichia coli, Streptococcus pneumonia and Staphlococcus aureus were used in this study, applying agar diffusion methods. The phytochemical screening indicated presence of secondary metabolites such as alkaloid, flavonoids, anthraquinoines, tannins and phenol in both extracts making it to have antibacterial potentials. Both extract showed remarkable activity against the growth of the selected bacteria; nevertheless, the methanol extract had more antibacterial activity than the water extract, more so the extracts were discovered to be more active at higher concentration. The water extract was not active at low concentration, that is 1:1 but had diameter zone of inhibition of 10 mm each for 1:2 concentration. The minimum inhibition concentration (MIC) that inhibits these bacterial ranged between 1:4 and 1:16 and the minimum bactericidal concentration (MBC) that kills the growth of the bacterial isolates completely was 1:16. The result of this study showed that M. oleifera could be a valuable antibacterial drug in the treatment of infections caused by the test organisms.
 
 
Key words: Agar diffusion method, aqueous and methanol extracts, secondary metabolites, zone of inhibition, minimum inhibition concentration (MIC), minimum bactericidal concentration (MBC).
 

Abalaka ME, Daniyan SY, Oyeleke SB, Adeyemo SO (2012). The antibacterial evaluation of Moringa oleifera leaf extract on selected bacterial pathogens. J. Microbiol. Res. 2(2):1-4
Crossref

 

Afolabi CA, Ibukun EO, Afor E, Obuofor EM, Farombi EO (2007). Phytochemical constituent and antioxidant activity of extracts from the leaves of Ocimum gratissimum. Sci. Res. Essay 2(5):163-166

 
 

Afolabi FE (2007). Chemical composition and antibacterial activity of Gongrenema latifolium. J. Zhejilang Univ. Sci. 8(5):352-358.
Crossref

 
 

Aiyegoro OA, Akinpelu DA, Afolayan AJ, Okoh AI (2008). Antibacterial activities of crude stem bark extracts of Distemonathus benthamianus Baill. J. Biol. Sci. 8(2):356-361.
Crossref

 
 

Akinyemi KO, Oladapo O, Okwara CE, Ibe CC, Fasure KA (2005). Screening of crude extracts of six medicinal plants used in South-west Nigerian unorthodox medicine for antimethicillin resistant S. aureus activity. BMC Complement. Altern. Med. 5:6-8.
Crossref

 
 

Anwar F, Sajib L, Muhammed A, Anwarul HG (2007). Moringa oleifera, a food plant with multiple medicinal uses. Phytol. Res. 21:17-25
Crossref

 
 

Ashafa AOT, Afolayan AJ (2009). Screening the root extract from Biden pilosa L. Var. radiate (Asteraceae) for antimicrobial potentials. J. Med. Plant Res. 3(8):568-572.

 
 

Boussaada O, Ammar S, Saidana D, Chriaa J, Chraif J, Daami M, Helel AN, Mighri Z (2008). Chemical composition and antimicrobial activity of volatile components from captitula and aerial parts of Rhaponticum acaule DC growing wild in Tunisia. Microbial. Res. 163:87-95.
Crossref

 
 

Brisibe EA, Umoren UE, Brisibe F, Magalhaes PM, Ferreira JFS, Luthria D, Wu X, Prior RL (2009). Nutritional characterization and antioxidant capacity of different tissues of Artemisia annua L. Food Chem. 115:1240-1246.
Crossref

 
 

Bukar A, Uba A, Oyeyi TI (2010). Antimicrobial profile of extract Lam extract against some food-borne microorganisms. Bayero J. Pure Appl. Sci. 3(1):43-48.

 
 

Kawo AH (2007). Water purification potentials and in vivo toxicity evaluation of the aqueous and petroleum either of catotropisprocera (Ait.F) Ait.F Latex and Moringa oleifera lam seed powder. PhD thesis, Microbiology Unit, Department of Biological sciences, Bayero University Kano. P 184.

 
 

Mahalingam R, Bharathidasan R, Ambikapathy V, Panneerselvam A (2011). Study on antibacterial activity of some medicinal plant against human pathogenic microorganism. Asian J. Plant Sci. Res. 1(3):86-90.

 
 

Mahesh B, Satish S (2008). Antimicrobial activity of some important medicinal plant against plant and human pathogens.World j. Agric. Sci. 4(5):839-843.

 
 

Makanjuola OO, Dada EO Ekundayo FO (2013). Antibacterial activities of Moringa oleifera (Lam) on coliforms isolated from some surface water in Akure Nigeria. FUTA J. Res. Sci. (1):63-71.

 
 

Moyo B, Masika PJ, Muchenje V (2012). Antimicrobial activities of Moringa oleifera lam Leaf extract. Afr. J. Biotechnol. 11(11):2797-2802.
Crossref

 
 

Parekh J, Chanda SV (2007). In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turk. J. Biol. 31:53-58.

 
 

Parekh J, Jadeja D, Chanda S (2005). Efficacy of aqueous and methanol extracts of some medicinal plants for potential antibacterial activity. Turk. J. Biol. 29:203-210.

 
 

Preethi RM Devanathan VV, Longanathan M (2010). Antimicrobial and antioxidant efficacy of some medicinal plant against food borne pathogens. Adv. Biol. Res. 4:122-125.

 
 

Schneider G, Wolfhing J (2004). Sherriesmedical microbiology. An introduction to infectious disease 4th ed. pp. 261-273.

 
 

Taylor JLS Van Staden J (2001). The effect of age season and growth conditions on anti-inflammatory activity in Eucomisautumnalis(mill). Chitt.plant extracts. Plant Growth Regul. 34(1):39-47.
Crossref

 
 

Theophilus UO, Omojate GC Anowi CF (2013). Phytochemical screening and investigation of antibacterial activities of various fractions of the ethanol leaves extract of Moringa oleifera Lam (Moringaceae). J. Pharm. Chem. Biol. Sci. 3(3):962-973.

 
 

Thilza IB, Sanni S, Zakari AI, Sanni FS, Muhammed T, Musa BJ (2010). In vito antimicrbial activity of water extract of Moringao leifera leaf stalk on bacteria normally implicated in eye diseaces. Acad. Arena. 2(6):80-82.

 
 

Voravuthikunchai S, Lortheeranuwat A, Jeeju W, Sririrak T, Phongpaichit S, Supawita T (2004). Effective medicinal plants against Enterohaemorrhagic Escherichia coli O157,H7. J. Ethanopharmacol. 94:49-54.
Crossref

 

 


APA Abubakar, I., & Usman, A. (2016). Phytochemical and antibacterial investigations of moringa (Moringa oleifera) leaf extract on selected bacterial pathogens. Journal of Microbiology and Antimicrobials, 8(5), 28-33.
Chicago Abubakar Idris and Usman Abubakar. "Phytochemical and antibacterial investigations of moringa (Moringa oleifera) leaf extract on selected bacterial pathogens." Journal of Microbiology and Antimicrobials 8, no. 5 (2016): 28-33.
MLA Abubakar Idris and Usman Abubakar. "Phytochemical and antibacterial investigations of moringa (Moringa oleifera) leaf extract on selected bacterial pathogens." Journal of Microbiology and Antimicrobials 8.5 (2016): 28-33.
   
DOI 10.5897/JMA2016.0361
URL http://academicjournals.org/journal/JMA/article-abstract/45083DB59795

Subscription Form