Journal of Microbiology and Antimicrobials
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Article Number - 70AB0E062123


Vol.8(6), pp. 34-38 , December 2016
DOI: 10.5897/JMA2016.0373
ISSN: 2141-2308



Full Length Research Paper

Antimicrobial analysis of copaiba oil extract from Passiflora cincinnata and endodontic substances



Lucinea Barbosa de Oliveira Santos
  • Lucinea Barbosa de Oliveira Santos
  • Department of Restorative Dentistry, Faculty of Dentistry, UFAL -Federal University of Alagoas, Maceió, AL, Brazil.
  • Google Scholar
Caroline de Souza Leitao
  • Caroline de Souza Leitao
  • Department of Restorative Dentistry, Faculty of Dentistry, UFAL -Federal University of Alagoas, Maceió, AL, Brazil.
  • Google Scholar
Amaro de Mendonca Cavalcante
  • Amaro de Mendonca Cavalcante
  • Department of Restorative Dentistry, Faculty of Dentistry, UFAL -Federal University of Alagoas, Maceió, AL, Brazil.
  • Google Scholar
Marcos Aurelio Bomfim da Silva
  • Marcos Aurelio Bomfim da Silva
  • Department of Restorative Dentistry, Faculty of Dentistry, UFAL -Federal University of Alagoas, Maceió, AL, Brazil.
  • Google Scholar
Zenaldo Porfirio
  • Zenaldo Porfirio
  • Department of Microbiology, university of Health Science of Alagoas,Maceio,Alagoas,Brazil
  • Google Scholar
Antonio Euzebio Goulart Santana
  • Antonio Euzebio Goulart Santana
  • Institute of Chemistry and Biotechnology,UFAL-Federal University of Alagoas,Maceio,Al,Brazil.
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 Received: 22 August 2016  Accepted: 04 November 2016  Published: 31 December 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The aim of this study was to evaluate the antimicrobial activity and minimum inhibitory concentration (MIC) of copaiba oil, extracts of Passiflora cincinnata and substances commonly used against endodontic infections of bacterial strains certified by the American Type Culture Collection (ATCC) and against clinical isolates (CI). The methodology involved the preparation of crude extracts of the plants, the selection of copaiba oil and the standardization of samples. The antibacterial activity of these substances was tested against Enterococcus faecalis (ATCC), Escherichia coli (CI) and Pseudomonas aeruginosa (ATCC and CI). The MIC was determined by broth dilution and the nitroblue tetrazolium chloride dye reduction test. The data were statistically analyzed by the Kolmogorov-Smirnov normality test and by the Kruskal-Wallis test with a confidence level of 95%. The analysis of the antimicrobial activity showed that the ethanol extracts of P. cincinnata and the combination of calcium hydroxide with polyethylene glycol with and without camphorated monochlorophenol showed no antimicrobial activity. However, the copaiba oil and other substances evaluated showed some antimicrobial activity against the microorganisms used (p <0.01), exhibiting a MIC ranging from <0.3 to >400 µL/mL (p <0.05). Copaiba oil showed antimicrobial activity and could represent a potential phytotherapeutic agent to be used against microorganisms causing endodontic infections.

Key words: Endodontics, Enterococcus faecalis, Passiflora cincinnata, Pseudomonas aeruginosa.

Andrews JM (2001). Determination of minimum inhibitory concentrations. J. Antimicrob. Chemother. 48:5-16.
Crossref

 

Bauer AW, Kirby WM, Sherris JC, Turck M (1966). Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45:493-496.

 

Birner J, Nicolls J.M (1973). Passicol, an antibacterial and antifungal agent produced by passiflora plant species: preparation and physicochemical characteristics. Antimicrob. Agents Chemother. 3:105-109.
Crossref

 

Costa EMMB, Esmeraldo MRA, Carvalho MGF, Daniel RLAP, Pastro MF, Silva Júnior FL (2008). Evaluation of the Antimicrobial Action of Propolis and Substances Used in Endodontics against Enterococcus faecalis. Pesq. Bras. Odontoped. Clin. Integr. 8:21-25.

 

Dianat O, Saedi S, Kazem M, Alam M (2015). Antimicrobial Activity of Nanoparticle Calcium Hydroxide against Enterococcus Faecalis: An In Vitro Study. Iran Endod J. 10:39-43.

 

Drumond MRS, Castro RD, Almeida RVD, Pereira MSV, Padilha WWN (2004). Comparative Study in vitro of the Antibacterial Activity from Phytotherapeutic Products Against Cariogenic Bacterias. Pesq. Bras. Odontoped. Clin. Integr. 4:33-38.

 

Estrela C, Bammann LL, Estrela CRA, Silva RS, Pécora JD (2000). Antimicrobial and Chemical Study of MTA, Portland Cement, Calcium Hydroxide Paste, Sealapex and Dycal. Braz. Dent. J.11:3-9.

 

Estrela C, Estrela CRA, Pecora JD (2003a). A study of the time necessary for calcium hydroxide to eliminate microorganisms in infected canals. J. Appl. Oral Sci. 11:133-137.
Crossref

 

Estrela C, Pimenta FC, Ito IY, Bammann LL (1998). In vitro determination of direct antimicrobial effect of calcium hydroxide. J. Endod. 24:15-17.
Crossref

 

Estrela C, Ribeiro RG, Estrela CRA, Pécora DJ, Sousa-Neto MD (2003b). Antimicrobial effect of 2% sodium hypochlorite and 2% chlorhexidine tested by different methods. Braz. Dent. J. 14:58-62.
Crossref

 

Ferreira FBA, Torres SA, Rosa OPS, Ferreira CM, Garcia RB, Marcucci MC, Gomes BPFA (2007). Antimicrobial effect of propolis and other substances against selected endodontic pathogens. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 104:709-716.
Crossref

 

Gales AC, Pignatari AC, Jones RN, Baretta M, Sader HS (1997). Evaluation of in vitro activity of new fluoroquinolones, cephalosporins and carbapenems against 569 gram-negative bacteria. Rev. Assoc. Med. Bras. 43:137-44.
Crossref

 

Gomes BPFA, Ferraz CCR, Vianna ME, Rosalen PL, Zaia AA, Teixeira FB, Souza-Filho FJ (2002). In vitro antimicrobial activity of calcium hydroxide pastes and their vehicles against selected microorganisms. Braz. Dent. J.13:155-161.
Crossref

 

Lima MRF, Luna JS, Santos AF, Andrade MCC, Sant'ana AEG, Genet JP, Marquez B, Neuville L, Moreau N (2006). Anti-bacterial activity of some Brazilian medicinal plants. J. Ethnopharmacol. 105:137-147.
Crossref

 

Melo ABP, Albuquerque DS, Castro CMMB (2004). Comparative study in vitro antimicrobial capacity of tricresol. J. Bras. Endod. 17:126-131.

 

Mendonça DE, Onofre SB (2009). Antimicrobial activity of the oil-resin produced by copaiba copaiferamultijugaHayne (Leguminosae). Rev. Bras. Farmac. 19:577-581.
Crossref

 

Nicolls JM, Birner J, Forsell P (1973). Passicol an antibacterial and antifungal agent produced by Passiflora Plant Species: Qualitative and Quantitative Range of Activity. Antimicrob. Agents Chemother. 3:110-117.
Crossref

 

Ohara P, Torabinejad M, Kettering JD (1993). Antibacterial effects of various endodontic medicaments on selected anaerobic bacteria. J. Endod. 19:498-500.
Crossref

 

Orstavik D, Haapasalo M (1990). Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules. Endod. Dent. Traumatol. 6(4):142-9.
Crossref

 

Packer JF, Luz MMS (2007). Evaluation and research method for natural products inhibitory activity. Rev. Bras. Farmacogn. 17:102-107.
Crossref

 

Pieri FA, Mussi MCM, Fiorini JE, Moreira MAS, Schneedorf JM (2012). Bacteriostatic Effect of Copaiba Oil (Copaifera officinalis) against Streptococcus mutans. Braz. Dent. J. 23:36-38.
Crossref

 

Pimenta HC, Violante IM, Musis CR, Borges ÁH, Aranha AM (2015). In vitro effectiveness of Brazilian brown propolis against Enterococcus faecalis. Bras. Oral Res. 29:1-6.
Crossref

 

Sader HS, Mendes RE, Gales AC, Jones RN, Pfaller MA, Zoccoli C, Sampaio J (2001). Antimicrobial susceptibility of bacteria isolated from the lower respiratory tract of inpatients with pneumonia in Brazilian hospitals – Results from the SENTRY surveillance program, 1997 and 1998. J. Pneumol. 27:59-67.
Crossref

 

Santos AO, Ueda-Nakamura T, Dias Filho BP, Veiga Jr VF, Pinto AC, Nakamura CV (2008). Antimicrobial activity of Brazilian copaiba oils obtained from different species of the Copaiferagenus. Mem. Inst. Oswaldo Cruz. 3:277-281.
Crossref

 

Silva KP, Irala LED, Salles AA, Limongi O, Soares RG (2006). Antimicrobial Activity Evaluation of Tricresol Formalin Opposite of Enterococcus Faecalis and Bacillus Subtilis. Rev. Endod. Pesq. Ensi On Line 4:1-11.

 

Siqueira Jr JF, Lopes HP (1999). Comparative study of the antimicrobial activity of drugs intra-canals through direct contact and distance. UFES Rev. Odontol. 1:60-64.

 

Veiga VF, Jr, Zunino L, Calixto JB, Patitucci ML, Pinto AC (2001). Phytochemical and antioedematogenic studies of commercial copaiba oils available in Brazil. Phytother. Res. 15:476-80.
Crossref

 

Wolfman C, Viola H, Paladini A, Dajas F, Medina JH (1994). Possible anxiolytic effects of chrysin, a central benzodiazepine receptor ligand isolated from Passiflora coerulea. Pharmacol. Biochem. Behav. 2(47):1-4.
Crossref

 


APA Santos, L. B. de O., Leitao, C. de S., Cavalcante, A. de M., da Silva, M. A. B., Porfirio, Z., Santana, A. E. G. (2016). Antimicrobial analysis of copaiba oil extract from Passiflora cincinnata and endodontic substances. Journal of Microbiology and Antimicrobials, 8(6), 34-38.
Chicago Lucinea Barbosa de Oliveira Santos,Caroline de Souza Leitao,Amaro de Mendonça Cavalcante,Marcos Aurelio Bomfim da Silva,Zenaldo Porfirio,Antonio Euzebio Goulart Santana. "Antimicrobial analysis of copaiba oil extract from Passiflora cincinnata and endodontic substances." Journal of Microbiology and Antimicrobials 8, no. 6 (2016): 34-38.
MLA Lucinea Barbosa de Oliveira Santos, et al. "Antimicrobial analysis of copaiba oil extract from Passiflora cincinnata and endodontic substances." Journal of Microbiology and Antimicrobials 8.6 (2016): 34-38.
   
DOI 10.5897/JMA2016.0373
URL http://academicjournals.org/journal/JMA/article-abstract/70AB0E062123

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