Journal of Microbiology and Antimicrobials
Subscribe to JMA
Full Name*
Email Address*

Article Number - 7186ED065362


Vol.9(3), pp. 23-34 , July 2017
https://doi.org/10.5897/JMA2015.0336
ISSN: 2141-2308


 Total Views: 0
 Downloaded: 0

Full Length Research Paper

Elevated temperature induced resistance markers’ reduction of some selected clinical bacterial pathogens



Otajevwo F. D.
  • Otajevwo F. D.
  • Department of Microbiology and Biotechnology, Western Delta University, Oghara, Nigeria.
  • Google Scholar
Otasowie O. F.
  • Otasowie O. F.
  • Department of Microbiology and Biotechnology, Western Delta University, Oghara, Nigeria.
  • Google Scholar







 Received: 09 January 2015  Accepted: 14 July 2016  Published: 31 July 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Axenic cultures of Klebsiella pneumoniae, Escherichia coli and Staphylococcus aureus obtained from the Medical Microbiology Laboratory of the Delta State University Teaching Hospital, Oghara, were inoculated into ten-fold serial dilutions of 10-1 to 10-7 in sterile Nutrient broth and incubated in water bath at 37°C for 18 h initially. The turbid broth cultures of 10-5, 10-6 and 10-7 dilutions were subcultured aseptically on sterile MacConkey agar, Nutrient agar and Blood agar plates and incubated at 37°C for 18 h. Antibiotic sensitivity testing was done using gentamycin, nalidixic acid, nitrofurantoin, ofloxacin, cotrimoxazole, tetracycline, amoxicillin and amoxicillin/clavulanic acid on isolates from the last three dilutions selected and results were recorded as before temperature treatment. Serial dilutions were repeated and the last three dilutions were subcultured on same media as before treatment and all inoculated plates were incubated at 30, 35, 40 and 45°C in the water bath for 18 h. Antibiotic sensitivity testing was then carried out using the same drugs and results were recorded as after treatment. Mean±standard error zones of inhibition (ZI) recorded for 10-5, 10-6 and 10-7 E. coli broth dilutions at 37°C by gentamycin, nalidixic acid, nitrofurantoin and ofloxacin were 13.8±10.0, 16.8±11.0, and 14.0±8.1 mm, respectively. Same broth dilutions of K. pneumoniae recorded 17.6±9.5, 14.4±6.0 and 13.8±9.3 mm ZI, respectively to same drugs. Similarly, same broth culture dilutions of S. aureus recorded 3.4±7.1, 2.8±6.9 and 2.8±5.7 mm ZI, respectively to same antibiotics. There was resistance reduction (RR) of 76.2±11.6 and 57.0±14.3% by 10-6 and 10-5 broth dilutions of E. coli at 30 and 40°C, respectively. In all three broth culture dilutions of K. pneumoniae, there was a less than 30% RR at all tested temperatures. S. aureus broth dilutions of 10-5, 10-6 and 10-7 recorded 173.9±6.4, 150.0±7.3 and 87.0±6.2% RR, respectively to ofloxacin at 30°C.  At 35°C, 10-7 S. aureus broth dilution recorded 87.0±4.3% RR. There was more than 50% RR in 10-5 and 10-6 S. aureus broth dilutions at 40°C and more than 50% RR in all three dilutions at 45°C. Vertically, the three pathogens in 10-7 broth dilution and at 40°C recorded a common RR of 58.1±11.2% against gentamicin. Broth culture dilutions of 10-5 and 10-6 of all pathogens produced RR of 74.6±22.2 and 74.6±21.0%, respectively against ofloxacin with no significant RR against the other drugs at the temperatures tested. The implications of these findings are discussed.

Key words: Elevated, temperature, resistance reduction, clinical pathogens.

Akortha EE, Filgona J (2009). Transfer of gentimicin resistance genes among enterobacteriaceae isolated from the outpatients with urinary tract infections attending 3 hospitals in Mubi, Adamawa State. Sci. Res. Essays 4(8):745-752.

 

Asheshov EH (1966). Loss of antibiotic resistance in Staphylococcus aureus resulting from growth at high temperature. J. Gen. Microbiol. 42(3):403-410.
Crossref

 

Asheshov EH (1966). Chromosomal location of the genetic element controlling Penicillinase production in a strain of Staphylococcus aureus. Nature 210(5038):804-806.
Crossref

 

Bauer AW, Kirby WM, Sherris JC, Turk M (1966). Antibiotic susceptibility testing by a standardized single disc method. Am. J. Clin. Pathol. 45:493-496.

 

Buchanan RL, Kawitter LA (1992). The effect of incubation temperature,Initial pH and sodium chloride on the growth kinetics of Escherichia coli 0157:h7. Food Microbiol. 9:185-196.
Crossref

 

Chakrabartty PK, Mishra AK, Chakrabarti SK (1984). Loss of plasmid Linked drug resistance after treatment with iodo-deoxyuridine. Indian J. Exp. Biol. 22:333-334.

 

Degerman R, Dinasquet J, Riemann L, de Luna SS, Andersson A (2013). Effect of resource availability on bacterial community responses to increased temperature. Sci. Rep. 68:131-142.
Crossref

 

Fairbrother RW, Parker L, Eaton BR (1954). The stability of penicillinase producing strains of Staphylococcus aureus. J. Gen. Microbiol. 10:309-314.
Crossref

 

Goller C, Romeo T (2008). Environmental influences on biofilm development. Curriculum Top. Microbiol. Immunol. 322:37-66.
Crossref

 

Gupta TD, Bandyopathy T, Dastidar SG, Bandopadhyay M, Mistra A, Chakrabarty AN (1980). R plasmids of Staphylococcus and their Elimination by different agents. Indian J. Exp. Biol. 18:478-481.

 

Hajdu S, Lassnigg A, Graninger W, Hirschl AM, Presterl E (2009). Effect of vancomycin, daptomycin, fosfomycin, tigecycline and ceftriaxone on Staphylococcus epidermidis biofilms. J. Orthop. Res. 27:136-1365.
Crossref

 

Hardy K (1986). Bacterial plasmids. Second edition. American Society for Microbiology. 1913 street N, W. Washington D.C. (20006) USA.

 

Kheder AK (2002). Studies on antibiotic resistance by plasmid of Pseudomonas aeruginosa Ph.D. thesis. College of Education. University of Salahaddin, Irag.

 

Kirchman DL, Rich JH (1997). Regulation of bacterial growth rates by Dissolved organic carbon and temperature in the equatorial Pacific Ocean. Microb. Ecol. 33:11-20.
Crossref

 

Lakshmi VV, Padma S, Polasa H (1989). Loss of plasmid antibiotic resistance in Escherichia coli on treatment with some compounds. FEMS Microbiol. Lett. 57:275-278.
Crossref

 

Madigan M, Martinko J, Parker J (2003). Brock biology of microorganisms (10th edition). Prentice Hall, Upper Saddle River, NJ. USA. 500 p.

 

May JW, Houghton RH, Perret CJ (1964). The effect of growth at elevated temperatures on some heritable properties of Staphylococcus aureus. J. Gen. Microbiol. 2:56-61.
Crossref

 

McGowan AP, Wise R (2001). Establishing MIC breakpoints and the Interpretation of invitro susceptibility tests. J. Antimicrob. Chemother. 48:17-28.
Crossref

 

Neu HC (1989). Overview of mechanisms of bacterial resistance. Diagn. Microbiol. Infect. Dis. 12:109-116.
Crossref

 

Obaseki-Ebor EE (1984). Rifampicin curing of plasmids in Escherichia coli k12 rifampicin resistant host. J. Pharm. Pharmacol. 36:467-470.
Crossref

 

Ochei J, Kolhatka A (2008). Medical Laboratory Science Theory and Practice. 10th Edn. New Delhi: Tata Mc Graw hill Publishing Company Limited. 255 p.

 

Okeke IN, Lamikanra A, Edelman R (1999). Socio economic and behavioural factors leading to acquired bacterial resistance to antibiotics in developing countries. Emerg. Infect. Dis. 5:18-27.
Crossref

 

Oskay M, Oskay D, Kalyoneu F (2009). Activity of some plant extracts against multidrug resistant human pathogens. Iran. J. Pharm. Res. 8(4):293-300.

 

Rachid S, Ohlsen K, Wallner U, Hacker J, Hecker M, Ziebuhr W (2000). Alternative transcription factor is involved in regulation of biofilms. Bacteriology 182:6824-6826.
Crossref

 

Radi OR, Rahman FH (2010). Study of the effects of ethidium bromide, SDS and elevated temperature on stability of multiple antibiotic resistance plasmids of Pseudomonas aeruginosa. Iraqi J. Biotechol. 9(4):797-811.

 

Ramesh A, Heami PM, Chandrasekhar A (2000). Ascorbic acid induced loss of a pediocin- encoding plasmid in Pediococus acidilactivi CFR K7. World J. Microbiol. Biotechnol.16:695-697.
Crossref

 

Reddy G, Shridhar P, Polasa H (1986). Elimination of Col. El (PBR322) and (PBR329) plasmids in Escherichia coli on treatment with hexamine ruthenium III chloride. Curr. Microbiol. 13:243-246.
Crossref

 

Stanier RY, Adelberg EA, Ingraham JL (1984). General Microbiology. 4th edition. The Placmilian Press LTD, London and Basingstoke.

 

Trevors JT (1986). Plasmid curing in bacteria. FEMS Microbiol. Rev. 32:149-152.
Crossref

 

Viljanen P, Boratynski J (1991). The susceptibility of conjugative resistance transfer in gram negative bacteria to physiochemical and biochemical agents. FEMS Microbiol. Rev. 88:43-45.
Crossref

 

Zhao BY, Hu KA, Wu RJ (2001). Primary study on the phenollic modification of sodium lignosulphate. Polym. Mater. Sci. Eng. 16(1):158-161.

 

Zielenkiewicz U, Ceglowski P (2001). Mechanisms of plasmids stable Maintainance with special focus on plasmid addiction system. Acta Biochem. Pol. 48(4):1003-1023.

 


APA Otajevwo, F. D., & Otasowie, O. F. (2017). Elevated temperature induced resistance markers’ reduction of some selected clinical bacterial pathogens. Journal of Microbiology and Antimicrobials, 9(3), 23-34.
Chicago Otajevwo F. D. and Otasowie O. F.. "Elevated temperature induced resistance markers’ reduction of some selected clinical bacterial pathogens." Journal of Microbiology and Antimicrobials 9, no. 3 (2017): 23-34.
MLA Otajevwo F. D. and Otasowie O. F.. "Elevated temperature induced resistance markers’ reduction of some selected clinical bacterial pathogens." Journal of Microbiology and Antimicrobials 9.3 (2017): 23-34.
   
DOI https://doi.org/10.5897/JMA2015.0336
URL http://academicjournals.org/journal/JMA/article-abstract/7186ED065362

Subscription Form