Journal of Microbiology and Antimicrobials
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Article Number - B9757829602


Vol.1(2), pp. 027-034 , November 2009

ISSN: 2141-2308



Full Length Research Paper

Prevalence of toxigenic traits in native food isolates of Bacillus cereus in the city of Mysore, Southern India


Shivalingsarj V. Desai and Mandyam C. Varadaraj*




Human Resource Development, Central Food Technological Research Institute (Council of Scientific and Industrial Research), Mysore 570 020, India.


Email: varadaraj@cftri.res.in






 Accepted: 09 November 2009  Published: 30 November 2009

Copyright © 2009 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


In the growing concern for microbial food safety of traditional foods, the present study has attempted to characterize toxigenic profile of native food isolates ofBacillus cereus. In a total of 65 traditional foods, 26 isolates were characterized by morphological, cultural and biochemical attributes as B. cereus. Of these, 12 isolates (46%) were confirmed as B. cereus by PCR with 16S rDNA and phosphatidylinositol phospholipase C primers in PCR. Among B. cereus isolates, 8 (67%) and 6 (50%) were positive for binding and lytic components of haemolysin (hbl complex) and sphingomyelinase (sph) in PCR. Besides, 67% of the isolates exhibited discontinuous haemolytic pattern in blood agar. The toxigenic food isolates of B. cereus CFR 1529, 1530, 1534 and 1536 revealed a high degree of sequence homology with selected strains of B. cereus and other species of B. cereus cluster as evidenced in sequence homology of partial nucleotide sequences of respective PCR amplicons of selected target genes. The study does indicate that toxigenic traits appear to be well spread within B. cereus cluster and have become stable traits among food isolates of B. cereus prevalent in the food chain.

Key words: Bacillus cereus, PCR, phosphatidylinositol phospholipase C, haemolysin BL, sphingomyelinase, toxigenic, sequence homology, microbial diversity.

 


APA (2009). Prevalence of toxigenic traits in native food isolates of Bacillus cereus in the city of Mysore, Southern India. Journal of Microbiology and Antimicrobials, 1(2), 027-034.
Chicago Shivalingsarj V. Desai and Mandyam C. Varadaraj. "Prevalence of toxigenic traits in native food isolates of Bacillus cereus in the city of Mysore, Southern India." Journal of Microbiology and Antimicrobials 1, no. 2 (2009): 027-034.
MLA Shivalingsarj V. Desai and Mandyam C. Varadaraj. "Prevalence of toxigenic traits in native food isolates of Bacillus cereus in the city of Mysore, Southern India." Journal of Microbiology and Antimicrobials 1.2 (2009): 027-034.
   
DOI
URL http://academicjournals.org/journal/JMA/article-abstract/B9757829602

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