Journal of Microbiology and Antimicrobials
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Article Number - EE1342B46782


Vol.6(6), pp. 97-103 , August 2014
DOI: 10.5897/JMA2014.0318
ISSN: 2141-2308



Full Length Research Paper

Control of Fusarium wilt using biological agent Streptomyces sp.CPP-53 isolated from compost with plant growth promoting effect on tomato under greenhouse condition



Ranveer Kamal*
  • Ranveer Kamal*
  • Department of Biological Sciences, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, US Nagar, Uttarakhand 263145, India.
  • Google Scholar
A. K. Sharma
  • A. K. Sharma
  • Department of Biological Sciences, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, US Nagar, Uttarakhand 263145, India.
  • Google Scholar







 Received: 22 May 2014  Accepted: 01 August 2014  Published: 31 August 2014

Copyright © 2014 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


In the present investigation, four different composts were obtained from Supa Biotech (P) Ltd., Muketshwar and LRC, GBPUAT, Pantnagar, India. The highest actinomycetes community was obtained from CPP compost (67.18%), LRC (15.62%), CM (12.5 %) and VE (4.6%). A total of 48 actinomycetes isolated and examined, exhibited ability to degrade starch, solubilize phosphate, produce catalase and siderophores. Isolated actinomycetes strains were checked for their antagonistic potential against seed and soil borne plant pathogens: Fusarium oxysporum (Wilt), Colletotrichum truncatum (Anthracnose in soyabean), Colletotrichum capsici (Anthracnose in chilli) and Helminthosporium oryzae (Brown spot in rice). It restricted mycelium growth of all four pathogens: H. oryzae (61.53%), F. oxysporum (57.5%), C. truncatum (54.05%) and C. capsici (50%) under in vitro condition. A greenhouse study was performed to evaluate efficiency of CPP-53 for controlling disease incidence by F. oxysporum in tomato plants. Out of the four treatments in this experiment, significantly lowest disease severity and higher plant vigour was recorded when CPP-53 was inoculated as compared to the control plants. Observation proved the potential of strain CPP-53 under in vitro condition and as an amendment in soil leading to suppression of pathogenic effect and efficient biocontrol agent. 23S rDNA region of actinomycetes strains were sequenced and the most potent one, CPP-53 has 98% similarity with Streptomyces flavofuscus.

 

Key words: Siderophore production, wilt, disease severity, Streptomyces flavofuscus, 23S rDNA, biocontrol. 

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APA Ranveer, K., & Sharma, A. K. (2014). Control of Fusarium wilt using biological agent Streptomyces sp.CPP-53 isolated from compost with plant growth promoting effect on tomato under greenhouse condition. Journal of Microbiology and Antimicrobials, 6(6), 97-103.
Chicago Ranveer Kamal and A. K. Sharma. "Control of Fusarium wilt using biological agent Streptomyces sp.CPP-53 isolated from compost with plant growth promoting effect on tomato under greenhouse condition." Journal of Microbiology and Antimicrobials 6, no. 6 (2014): 97-103.
MLA Ranveer Kamal and A. K. Sharma. "Control of Fusarium wilt using biological agent Streptomyces sp.CPP-53 isolated from compost with plant growth promoting effect on tomato under greenhouse condition." Journal of Microbiology and Antimicrobials 6.6 (2014): 97-103.
   
DOI 10.5897/JMA2014.0318
URL http://academicjournals.org/journal/JMA/article-abstract/EE1342B46782

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