Journal of Medical Genetics and Genomics
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Article Number - 407E17A9026


Vol.5(1), pp. 1 - 5 , February 2013

ISSN: 2141-2278



Full Length Research Paper

Association of insertion/deletion polymorphism of Alu angiotensin converting enzyme insertion/deletion genotype with type 2 diabetes mellitus and hypertension in J&K population: A case control study


Rakesh K. Panjaliya*, Surbhi Sethi, Minakashee Sharma, Ravi Sharma, Parvinder Kumar and Subash Gupta




The objective of the study is to know the frequencies of insertion/deletion (I/D) allele and association of angiotensin converting enzyme (ACE), I/D polymorphism in Jammu and Kashmir (J&K) populations in relation to type 2 diabetes mellitus (T2DM) and hypertension (HTN). A total of 500 individuals were recruited for the present study. Out of these 500 individuals, 250 individuals had T2DM and HTN and 250 were healthy controls. Genotyping was performed using polymerase chain reaction (PCR) using allele specific oligonucleotide primers. The allele frequency for I allele and D allele was found to be 63% and 37% in patients with T2DM and HTN and 48% and 52% for healthy controls. Genotype frequency for homozygote insertion (II), heterozygote (ID) and homozygote deletion (DD) allele was in range of 99.23, 116.55 and 34.23 for patients with T2DM and HTN and 56.64, 124.71 and 68.64 for healthy controls. ID versus II+ID model for odds showed a significant association of ACE I/D polymorphism with T2DM and HTN. The I allele and ID genotype of ACE gene is associated with T2DM and HTN.

 

Key words:  ACE, insertion, deletion, type 2 diabetes mellitus (T2DM) and hypertension (HTN).


Email: rakesh_chinab@yahoo.co.in






 Accepted: 04 February 2013  Published: 28 February 2013

Copyright © 2013 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The objective of the study is to know the frequencies of insertion/deletion (I/D) allele and association of angiotensin converting enzyme (ACE), I/D polymorphism in Jammu and Kashmir (J&K) populations in relation to type 2 diabetes mellitus (T2DM) and hypertension (HTN). A total of 500 individuals were recruited for the present study. Out of these 500 individuals, 250 individuals had T2DM and HTN and 250 were healthy controls. Genotyping was performed using polymerase chain reaction (PCR) using allele specific oligonucleotide primers. The allele frequency for I allele and D allele was found to be 63% and 37% in patients with T2DM and HTN and 48% and 52% for healthy controls. Genotype frequency for homozygote insertion (II), heterozygote (ID) and homozygote deletion (DD) allele was in range of 99.23, 116.55 and 34.23 for patients with T2DM and HTN and 56.64, 124.71 and 68.64 for healthy controls. ID versus II+ID model for odds showed a significant association of ACE I/D polymorphism with T2DM and HTN. The I allele and ID genotype of ACE gene is associated with T2DM and HTN.

 

Key words:  ACE, insertion, deletion, type 2 diabetes mellitus (T2DM) and hypertension (HTN).


APA (2013). Association of insertion/deletion polymorphism of Alu angiotensin converting enzyme insertion/deletion genotype with type 2 diabetes mellitus and hypertension in J&K population: A case control study. Journal of Medical Genetics and Genomics, 5(1), 1 - 5.
Chicago Rakesh K. Panjaliya, Surbhi Sethi, Minakashee Sharma, Ravi Sharma, Parvinder Kumar and Subash Gupta. "Association of insertion/deletion polymorphism of Alu angiotensin converting enzyme insertion/deletion genotype with type 2 diabetes mellitus and hypertension in J&K population: A case control study." Journal of Medical Genetics and Genomics 5, no. 1 (2013): 1 - 5.
MLA Rakesh K. Panjaliya, et al. "Association of insertion/deletion polymorphism of Alu angiotensin converting enzyme insertion/deletion genotype with type 2 diabetes mellitus and hypertension in J&K population: A case control study." Journal of Medical Genetics and Genomics 5.1 (2013): 1 - 5.
   
DOI
URL http://academicjournals.org/journal/JMGG/article-abstract/407E17A9026

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