Full Length Research Paper
Fawzi Alsheyab1, Nizar Abuharfil1, Mohanad Aldarouish1, Dalal Awad2 and Yousef Mosameh3*
1Department of Biotechnology, Jordan University of Science and Technology (JUST), Irbid- Jordan.
2Department of Biological Sciences, Yarmouk University, Irbid-Jordan.
3Renad Medical Laboratory, Amman-Jordan.
Phosphodiesterase 5 (PDE5) is a catalytic enzyme for degradation of cyclic guanosine monophosphate (cGMP) in human smooth muscle cells. Inhibition of this enzyme by certain chemicals including sildenafil, vardenafil and tadalafil, is considered a breakthrough treatment of erectile dysfunction. Unfortunately, these drugs are associated with several side effects. In this study, another strategy is adopted in the inhibition of the PDE5 enzyme by using a monoclonal antibody (Mab) against the enzyme. The Mab was prepared by immunizing a mouse, taking the spleen to make single cell suspension of lymphocytes. Hybridoma cells were obtained by fusion of BALB/c myeloma cells with the immunized lymphocytes using the polyethylene glycol method. Several clones were produced that have been separated by limiting dilution method. The antibodies were tested by enzyme-linked immunosorbent assay (ELISA) and western blot. The obtained Mab has titer of 625, immunoglobulin G1 (IgG1) isotype and 3.9 × 104 mol-1 affinity constant.
Key words: Phosphodiesterase (PDE), chemical inhibition, monoclonal antibody (Mab).
|APA||(2013). Preparation and partial characterization of monoclonal antibody against human phosphdiesterase 5 (PDE5). Journal of Medical Genetics and Genomics, 5(1), 6 - 13.|
|Chicago||Fawzi Alsheyab, Nizar Abuharfil, Mohanad Aldarouish, Dalal Awad and Yousef Mosameh. "Preparation and partial characterization of monoclonal antibody against human phosphdiesterase 5 (PDE5)." Journal of Medical Genetics and Genomics 5, no. 1 (2013): 6 - 13.|
|MLA||Fawzi Alsheyab, et al. "Preparation and partial characterization of monoclonal antibody against human phosphdiesterase 5 (PDE5)." Journal of Medical Genetics and Genomics 5.1 (2013): 6 - 13.|