Journal of Medical Genetics and Genomics
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Article Number - CBC0F609054


Vol.5(1), pp. 6 - 13 , February 2013

ISSN: 2141-2278



Full Length Research Paper

Preparation and partial characterization of monoclonal antibody against human phosphdiesterase 5 (PDE5)


Fawzi Alsheyab1, Nizar Abuharfil1, Mohanad Aldarouish1, Dalal Awad2 and Yousef Mosameh3*




1Department of Biotechnology, Jordan University of Science and Technology (JUST), Irbid- Jordan.

2Department of Biological Sciences, Yarmouk University, Irbid-Jordan.

3Renad Medical Laboratory, Amman-Jordan.


Email: ymosameh@hotmail.com






 Accepted: 01 June 2012  Published: 28 February 2013

Copyright © 2013 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Phosphodiesterase 5 (PDE5) is a catalytic enzyme for degradation of cyclic guanosine monophosphate (cGMP) in human smooth muscle cells. Inhibition of this enzyme by certain chemicals including sildenafil, vardenafil and tadalafil, is considered a breakthrough treatment of erectile dysfunction. Unfortunately, these drugs are associated with several side effects. In this study, another strategy is adopted in the inhibition of the PDE5 enzyme by using a monoclonal antibody (Mab) against the enzyme. The Mab was prepared by immunizing a mouse, taking the spleen to make single cell suspension of lymphocytes. Hybridoma cells were obtained by fusion of BALB/c myeloma cells with the immunized lymphocytes using the polyethylene glycol method. Several clones were produced that have been separated by limiting dilution method. The antibodies were tested by enzyme-linked immunosorbent assay (ELISA) and western blot. The obtained Mab has titer of 625, immunoglobulin G1 (IgG1) isotype and 3.9 × 104 mol-1 affinity constant.

 

Key words: Phosphodiesterase (PDE), chemical inhibition, monoclonal antibody (Mab).


APA (2013). Preparation and partial characterization of monoclonal antibody against human phosphdiesterase 5 (PDE5). Journal of Medical Genetics and Genomics, 5(1), 6 - 13.
Chicago Fawzi Alsheyab, Nizar Abuharfil, Mohanad Aldarouish, Dalal Awad and Yousef Mosameh. "Preparation and partial characterization of monoclonal antibody against human phosphdiesterase 5 (PDE5)." Journal of Medical Genetics and Genomics 5, no. 1 (2013): 6 - 13.
MLA Fawzi Alsheyab, et al. "Preparation and partial characterization of monoclonal antibody against human phosphdiesterase 5 (PDE5)." Journal of Medical Genetics and Genomics 5.1 (2013): 6 - 13.
   
DOI
URL http://academicjournals.org/journal/JMGG/article-abstract/CBC0F609054

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