Journal of
Medical Laboratory and Diagnosis

  • Abbreviation: J. Med. Lab. Diagn.
  • Language: English
  • ISSN: 2141-2618
  • DOI: 10.5897/JMLD
  • Start Year: 2010
  • Published Articles: 51

Full Length Research Paper

Comparison of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) with conventional culture and biochemical method of bacteria identification to species level

Iroha Ifeanyichukwu Romanus
  • Iroha Ifeanyichukwu Romanus
  • Department of Applied Microbiology, Faculty of Biological Sciences Ebonyi State University, P.M.B. 053, Abakaliki, Ebonyi State, Nigeria.
  • Google Scholar
Ayogu Thomas Eze
  • Ayogu Thomas Eze
  • Department of Food Technology, Institute of Management and technology, Enugu, P.M.B. 01079, Enugu State, Nigeria.
  • Google Scholar
Oji Anthonia Egwu
  • Oji Anthonia Egwu
  • Department of Applied Microbiology, Faculty of Biological Sciences Ebonyi State University, P.M.B. 053, Abakaliki, Ebonyi State, Nigeria.
  • Google Scholar
Afiukwa Felicitas Ngozi
  • Afiukwa Felicitas Ngozi
  • Department of Applied Microbiology, Faculty of Biological Sciences Ebonyi State University, P.M.B. 053, Abakaliki, Ebonyi State, Nigeria.
  • Google Scholar
Nwuzo Agabus Chidiebube
  • Nwuzo Agabus Chidiebube
  • Department of Applied Microbiology, Faculty of Biological Sciences Ebonyi State University, P.M.B. 053, Abakaliki, Ebonyi State, Nigeria.
  • Google Scholar


  • Article Number - 9CB0A4C829
  • Vol.2(1), pp. 1-4, January 2011
  •  Accepted: 28 November 2010
  •  Published: 31 January 2011

Abstract

Identification of bacteria to species level is based on culturing in different media which takes 48 to 72 h from the time of isolation. Matrix-assisted desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a new technology in bacteria identification. We evaluated MALDI-TOF-MS system with 101 consecutively isolated bacteria colonies under routine laboratory conditions by comparing its identification efficiency with conventional culturing and biochemical tests. The MALDI-TOF MS identified bacteria colonies as Escherichia coli44(43.5%), Klebsiella pneumoniae  56(55.4%) and Stenotrophomonas maltophila 1(1.4%) while cultural and biochemical method identified  bacteria colonies as E. coli 43(42.5%), K. pneumoniae  56(55.4%) and failed to identify one bacteria colony which was identified as S. maltophila by MALDI-TOF MS. Our data suggests that although both methods are good for bacteria identification, MALDI-TOF MS is better than conventional culturing and biochemical tests because of its short turn around time with no loss of accuracy.

 

Key words: Bacteria identification, MALDI- TOF MS, culture, biochemical test, bacteria colony.