Journal of Medical Laboratory and Diagnosis
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Article Number - 9CB0A4C829


Vol.2(1), pp. 1-4 , January 2011

ISSN: 2141-2618



Full Length Research Paper

Comparison of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) with conventional culture and biochemical method of bacteria identification to species level



Iroha Ifeanyichukwu Romanus
  • Iroha Ifeanyichukwu Romanus
  • Department of Applied Microbiology, Faculty of Biological Sciences Ebonyi State University, P.M.B. 053, Abakaliki, Ebonyi State, Nigeria.
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Ayogu Thomas Eze
  • Ayogu Thomas Eze
  • Department of Food Technology, Institute of Management and technology, Enugu, P.M.B. 01079, Enugu State, Nigeria.
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Oji Anthonia Egwu
  • Oji Anthonia Egwu
  • Department of Applied Microbiology, Faculty of Biological Sciences Ebonyi State University, P.M.B. 053, Abakaliki, Ebonyi State, Nigeria.
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Afiukwa Felicitas Ngozi
  • Afiukwa Felicitas Ngozi
  • Department of Applied Microbiology, Faculty of Biological Sciences Ebonyi State University, P.M.B. 053, Abakaliki, Ebonyi State, Nigeria.
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Nwuzo Agabus Chidiebube
  • Nwuzo Agabus Chidiebube
  • Department of Applied Microbiology, Faculty of Biological Sciences Ebonyi State University, P.M.B. 053, Abakaliki, Ebonyi State, Nigeria.
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 Accepted: 28 November 2010  Published: 31 January 2011

Copyright © 2011 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Identification of bacteria to species level is based on culturing in different media which takes 48 to 72 h from the time of isolation. Matrix-assisted desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a new technology in bacteria identification. We evaluated MALDI-TOF-MS system with 101 consecutively isolated bacteria colonies under routine laboratory conditions by comparing its identification efficiency with conventional culturing and biochemical tests. The MALDI-TOF MS identified bacteria colonies as Escherichia coli44(43.5%), Klebsiella pneumoniae  56(55.4%) and Stenotrophomonas maltophila 1(1.4%) while cultural and biochemical method identified  bacteria colonies as E. coli 43(42.5%), K. pneumoniae  56(55.4%) and failed to identify one bacteria colony which was identified as S. maltophila by MALDI-TOF MS. Our data suggests that although both methods are good for bacteria identification, MALDI-TOF MS is better than conventional culturing and biochemical tests because of its short turn around time with no loss of accuracy.

 

Key words: Bacteria identification, MALDI- TOF MS, culture, biochemical test, bacteria colony.


APA (2011). Comparison of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) with conventional culture and biochemical method of bacteria identification to species level. Journal of Medical Laboratory and Diagnosis, 2(1), 1-4.
Chicago Iroha Ifeanyichukwu Romanus, Ayogu Thomas Eze, Oji Anthonia Egwu, Afiukwa Felicitas Ngozi and Nwuzo Agabus Chidiebube. "Comparison of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) with conventional culture and biochemical method of bacteria identification to species level." Journal of Medical Laboratory and Diagnosis 2, no. 1 (2011): 1-4.
MLA Iroha Ifeanyichukwu Romanus, et al. "Comparison of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) with conventional culture and biochemical method of bacteria identification to species level." Journal of Medical Laboratory and Diagnosis 2.1 (2011): 1-4.
   
DOI
URL http://academicjournals.org/journal/JMLD/article-abstract/9CB0A4C829

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