Journal of Medicinal Plants Research
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Article Number - 2788F9F64150


Vol.11(17), pp. 331-337 , May 2017
DOI: 10.5897/JMPR2017.6366
ISSN: 1996-0875



Full Length Research Paper

Optimization of enzyme assisted extraction of polysaccharides from Poria cocos



Ashfaque A. Khaskheli
  • Ashfaque A. Khaskheli
  • College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
  • Google Scholar
Shahzor G. Khaskheli
  • Shahzor G. Khaskheli
  • Institute of Food Sciences and Technology, Sindh Agriculture University, Tandojam 71000, Pakistan.
  • Google Scholar
Ying Liu
  • Ying Liu
  • College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
  • Google Scholar
Saghir A. Sheikh
  • Saghir A. Sheikh
  • Institute of Food Sciences and Technology, Sindh Agriculture University, Tandojam 71000, Pakistan.
  • Google Scholar
Yan-Feng Wang
  • Yan-Feng Wang
  • Mudanjiang Sub-Academy, Heilongjiang Academy of Agricultural Sciences, Mudanjiang, Heilongjiang, 157041, China.
  • Google Scholar
Aijaz H. Soomro
  • Aijaz H. Soomro
  • Institute of Food Sciences and Technology, Sindh Agriculture University, Tandojam 71000, Pakistan.
  • Google Scholar
Xiaojiu Tian
  • Xiaojiu Tian
  • College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
  • Google Scholar
Mamoun A. Homaida
  • Mamoun A. Homaida
  • College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
  • Google Scholar
Wen Huang*
  • Wen Huang*
  • College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
  • Google Scholar







 Received: 25 February 2017  Accepted: 06 April 2017  Published: 03 May 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Water-soluble polysaccharide was isolated from Poria cocus by enzyme-assisted method using orthogonal methodology. By single factor test and orthogonal group, the extraction conditions of water-soluble polysaccharide were investigated, such as liquid-solid ratio, temperature, time and pH. The optimum conditions for single factor were as follows Enzyme concentration (%), Extraction temperature oC, Extraction time/h, Extraction pH. The result revealed that 2% complex enzyme, remained most important factor of polysaccharide extraction, followed by temperature and pH. The liquid-solid ratio was 1:50, temperature was 40°C, time was 3.0 h, and pH was 5. The highest extraction rate of crude polysaccharide remained 4.14%. Results indicated that the complex-enzyme assisted remained best technique for extracting polysaccharide from P. cocus. It proved to be as highly effective as well as energy and time saving extraction techniques.

Key words: Poria cocus enzyme assisted extraction, polysaccharides, alpha amylase, cellulase, Taka-diastase.

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APA Khaskheli, A. A., Khaskheli, S. G., Liu, Y., Sheikh, S. A., Wang, Y., Soomro, A. H., Tian, X., Homaida, M. A., & Huang, W. (2017). Optimization of enzyme assisted extraction of polysaccharides from Poria cocos. Journal of Medicinal Plants Research, 11(17), 331-337.
Chicago Ashfaque A. Khaskheli, Shahzor G. Khaskheli, Ying Liu, Saghir A. Sheikh, Yan-Feng Wang, Aijaz H. Soomro, Xiaojiu Tian, Mamoun A. Homaida and Wen Huang. "Optimization of enzyme assisted extraction of polysaccharides from Poria cocos." Journal of Medicinal Plants Research 11, no. 17 (2017): 331-337.
MLA Ashfaque A. Khaskheli, et al. "Optimization of enzyme assisted extraction of polysaccharides from Poria cocos." Journal of Medicinal Plants Research 11.17 (2017): 331-337.
   
DOI 10.5897/JMPR2017.6366
URL http://academicjournals.org/journal/JMPR/article-abstract/2788F9F64150

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