Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3538

Full Length Research Paper

Optimization of enzyme assisted extraction of polysaccharides from Poria cocos

Ashfaque A. Khaskheli
  • Ashfaque A. Khaskheli
  • College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
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Shahzor G. Khaskheli
  • Shahzor G. Khaskheli
  • Institute of Food Sciences and Technology, Sindh Agriculture University, Tandojam 71000, Pakistan.
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Ying Liu
  • Ying Liu
  • College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
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Saghir A. Sheikh
  • Saghir A. Sheikh
  • Institute of Food Sciences and Technology, Sindh Agriculture University, Tandojam 71000, Pakistan.
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Yan-Feng Wang
  • Yan-Feng Wang
  • Mudanjiang Sub-Academy, Heilongjiang Academy of Agricultural Sciences, Mudanjiang, Heilongjiang, 157041, China.
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Aijaz H. Soomro
  • Aijaz H. Soomro
  • Institute of Food Sciences and Technology, Sindh Agriculture University, Tandojam 71000, Pakistan.
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Xiaojiu Tian
  • Xiaojiu Tian
  • College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
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Mamoun A. Homaida
  • Mamoun A. Homaida
  • College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
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Wen Huang*
  • Wen Huang*
  • College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
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  •  Received: 25 February 2017
  •  Accepted: 06 April 2017
  •  Published: 03 May 2017

Abstract

Water-soluble polysaccharide was isolated from Poria cocus by enzyme-assisted method using orthogonal methodology. By single factor test and orthogonal group, the extraction conditions of water-soluble polysaccharide were investigated, such as liquid-solid ratio, temperature, time and pH. The optimum conditions for single factor were as follows Enzyme concentration (%), Extraction temperature oC, Extraction time/h, Extraction pH. The result revealed that 2% complex enzyme, remained most important factor of polysaccharide extraction, followed by temperature and pH. The liquid-solid ratio was 1:50, temperature was 40°C, time was 3.0 h, and pH was 5. The highest extraction rate of crude polysaccharide remained 4.14%. Results indicated that the complex-enzyme assisted remained best technique for extracting polysaccharide from P. cocus. It proved to be as highly effective as well as energy and time saving extraction techniques.

Key words: Poria cocus enzyme assisted extraction, polysaccharides, alpha amylase, cellulase, Taka-diastase.