Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3538

Full Length Research Paper

Biological and antioxidant activity of Gunnera tinctoria (Nalca)

Pedro Zamorano
  • Pedro Zamorano
  • Graduate School, Faculty of Agricultural Science, Universidad Austral de Chile, Valdivia, Chile.
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Benjamín Rojano
  • Benjamín Rojano
  • Laboratorio Ciencia de los Alimentos, Facultad de Ciencias, Universidad Nacional de Colombia, Medellín, Colombia.
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Marcela Morales
  • Marcela Morales
  • Laboratorio Ciencia de los Alimentos, Facultad de Ciencias, Universidad Nacional de Colombia, Medellín, Colombia.
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Haroldo Magariños
  • Haroldo Magariños
  • Food Science and Technology Institute (ICYTAL), Faculty of Agricultural Sciences, Universidad Austral de Chile, Campus Isla Teja s/n, Valdivia, Chile.
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Patricio Godoy
  • Patricio Godoy
  • Clinical Microbiology Institute, Medicine Faculty, Universidad Austral de Chile, Valdivia, Chile.
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Ociel Muñoz*
  • Ociel Muñoz*
  • Food Science and Technology Institute (ICYTAL), Faculty of Agricultural Sciences, Universidad Austral de Chile, Campus Isla Teja s/n, Valdivia, Chile.
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  •  Received: 23 March 2017
  •  Accepted: 26 April 2017
  •  Published: 03 May 2017

Abstract

The endemic Chilean edible plant Gunnera tinctoria (Nalca) is highly appreciated in the south of Chile by the small farmers. Nevertheless, no background exists about his secondary metabolites. In the present study, in the leaf from G. tinctoria was investigated the content of bioactive compounds like coumaric acid, ascorbic acid and total phenols; the antioxidant capacity was evaluated by 3-ethylbenzothiazoline-6-suslfonic acid (ABTS), oxygen radical absorbance capacity (ORAC), ferric reducing antioxidant power), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods reducing sugars were measured. Finally, the biological activity was evaluated against Cladophialophora and Cryptococcus laurentii. The results suggest that the most abundant constituent in the extract were catechin (1344.97 mg/100 g dry weight) and epicatechin (1429.28 mg/100g dry weight), and was confirmed and quantified by high performance liquid chromatografy (HPLC-PDA); while the ORAC methodology showed a high antioxidant capacity (192000.0±5.91 umol Trolox Eq/100 g dry weight). On the other hand, the extract had a fungicide effect against both microorganism assayed, inhibiting the growth of Cladophialophora´s mold- and the yeast Cryptococcus laurentii. This is the first report of antioxidant capacity, bioactive compounds and biological activity of G. tinctoria, and these findings suggest that an extract prepared from the Nalca leaf may be a promising source of antioxidant and bioactive compounds and as a research object by being an antifungal and therapeutic alternative in development.

Key words: Gunnera tinctoria, Nalca, antioxidant capacity, biological activity, nutraceutical.