Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3545

Full Length Research Paper

Secondary metabolites from endophytic fungus from Lippia sidoides Cham.

Talita Pereira de Souza Ferreira*
  • Talita Pereira de Souza Ferreira*
  • Bioprocess Engineering and Biotechnology, Federal University of Tocantins, University Campus of Gurupi, 77410-530 Gurupi, TO, Brazil.
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Gil Rodrigues dos Santos
  • Gil Rodrigues dos Santos
  • Agronomic Engineering, Federal University of Tocantins, University Campus of Gurupi, 77410-530 Gurupi, TO, Brazil.
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Ilsamar Mendes Soares
  • Ilsamar Mendes Soares
  • Bioprocess Engineering and Biotechnology, Federal University of Tocantins, University Campus of Gurupi, 77410-530 Gurupi, TO, Brazil.
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Sergio Donizeti Ascencio
  • Sergio Donizeti Ascencio
  • Medicine, Federal University of Tocantins, University Campus of Palmas, 77001-090 Palmas, TO, Brazil.
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Tarso da Costa Alvim
  • Tarso da Costa Alvim
  • Food Engineering, Federal University of Tocantins, University Campus of Palmas, 77001-090 Palmas, TO, Brazil.
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Chrystian de Assis Siqueira
  • Chrystian de Assis Siqueira
  • Bioprocess Engineering and Biotechnology, Federal University of Tocantins, University Campus of Gurupi, 77410-530 Gurupi, TO, Brazil.
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Raimundo Wagner de Souza Aguiar
  • Raimundo Wagner de Souza Aguiar
  • Bioprocess Engineering and Biotechnology, Federal University of Tocantins, University Campus of Gurupi, 77410-530 Gurupi, TO, Brazil.
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  •  Received: 14 March 2017
  •  Accepted: 18 April 2017
  •  Published: 25 April 2017

Abstract

Lippia sidoides Cham. (Verbenaceae) is a species native to the Brazilian northeast, widely used in popular medicine. Its leaves were used for the isolation of endophytic fungi and extraction of metabolites. Among them, three were selected according to fungitoxicity tests against the maize phytopathogenic fungus, Curvularia lunata (Wakker). However, the objective of this study was to identify the role of L. sidoides extracts associated with their endophytic fungi, necessary to reduce excess of fungicides applied on the maize crop. Metabolites were evaluated for antioxidant activity by 2.2-diphenyl-1-picrylhydrazyl (DPPH), phenols, total flavonoids and one of it endophytic fungus 

were evaluated for synergism (Verticilium sp. and plant extracts). The endophytic fungi and plant extracts evaluated for phenolic content ranged from 0.29 ± 0.05 to 96.94 ± 11.86 mgEAG/g, the content of flavonoids from 14.31 ± 1.56 to 192.33 ± 4.58 mgER/g, and antioxidant activity could only be observed for the plant extract with EC50 81 ± 0.3%. The secondary metabolites identified by HPLC in the plant extract were catechin, quercetin, gallic acid and naringin. Naringenin, catechin, epigallocatechin gallate and quercetin were identified in the extract of the fungi viz. Verticillium sp. and Fusarium sp. Synergistic analysis between a 1:1 proportion of plant and fungal extracts has shown more efficient (79.0%) inhibition of C. lunata. Thus, alternative control of phytopathogenic fungi can be accomplished using plant extracts associated with their endophytic fungi, reducing the excess of fungicides applied on the maize crop.

Key words: Curvularia lunata, endophytic fungal, HPLC, extract, Verticillium sp., Fusarium sp., Colletrotrichum sp.