Journal of Medicinal Plants Research
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Article Number - A3BACEF14571


Vol.3(1), pp. 001-008 , January 2009

ISSN: 1996-0875



Full Length Research Paper

Anti-inflammatory activity of brown alga Dictyota dichotoma in murine macrophage RAW 264.7 cells


Weon-Jong Yoon1, Young Min Ham1, Kil-Nam Kim1, Soo-Yeung Park1, Nam Ho Lee2, Chang-Gu Hyun1* and Wook Jae Lee1*




1Research Group for Cosmetic Materials, Jeju Biodiversity Research Institute (JBRI) and Jeju Hi-Tech Industry Development Institute (HiDI), Jeju 697-943, Korea.

2Department of Chemistry, Cheju National University, Jeju 690-756, Korea.


Email: cghyun@jejuhidi.or.kr or algae@jejuhidi.or.kr






 Accepted: 26 November 2008  Published: 31 January 2009

Copyright © 2009 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


COX-2 (Cyclooxygenase-2) and iNOS (inducible nitric oxide synthase) are involved in various pathophysiological processes such as inflammation. In a search for inhibitors of COX-2 and iNOS, we found that extracts of Dictyota dichotoma inhibit Nitric oxide (NO) and PGE2 generation in LPS-stimulated RAW 264.7 macrophage cells, as well as levels of iNOS and COX-2 mRNA and protein.D. dichotoma were extracted with 80% EtOH. The extract was then partitioned with hexane, CH2Cl2, EtOAc, BuOH, and water, successively. The results indicate that the CH2Cl2 fraction of D. dichotoma extract is an effective inhibitor of LPS-induced NO and PGE2 production in RAW 264.7 cells. These inhibitory effects of the CH2Cl2 fraction of D. dichotoma were accompanied by decreases in the expression of iNOS and COX-2 proteins and iNOS and COX-2 mRNA in dose-dependent pattern. To test the inhibition effects of D. dichotoma fractions on other cytokines, we also performed RT-PCR on TNF-α, IL-1β, and IL-6 in LPS-stimulated RAW 264.7 macrophage cells. In these assays, the CH2Cl2 fraction ofD. dichotoma also showed decreases in the expression of TNF-α, IL-1β, and IL-6 mRNA. Based on these results, we suggest that D. dichotoma extracts may be considered possible anti-inflammatory candidates for human health.

 

Key words: COX-2, Cytokine, Dictyota dichotoma, inflammation, iNOS (inducible nitric oxide synthase), PGE2.


APA (2009). Anti-inflammatory activity of brown alga Dictyota dichotoma in murine macrophage RAW 264.7 cells. Journal of Medicinal Plants Research, 3(1), 001-008.
Chicago Weon-Jong Yoon, Young Min Ham, Kil-Nam Kim, Soo-Yeung Park, Nam Ho Lee, Chang-Gu Hyun and Wook Jae Lee. "Anti-inflammatory activity of brown alga Dictyota dichotoma in murine macrophage RAW 264.7 cells." Journal of Medicinal Plants Research 3, no. 1 (2009): 001-008.
MLA Weon-Jong Yoon, et al. "Anti-inflammatory activity of brown alga Dictyota dichotoma in murine macrophage RAW 264.7 cells." Journal of Medicinal Plants Research 3.1 (2009): 001-008.
   
DOI
URL http://academicjournals.org/journal/JMPR/article-abstract/A3BACEF14571

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