Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3553

Full Length Research Paper

Characterization of the bioactive constituents of Nymphaea alba rhizomes and evaluation of anti-biofilm as well as antioxidant and cytotoxic properties

Riham Omar Bakr
  • Riham Omar Bakr
  • Pharmacognosy Department, October University for Modern Sciences and Arts (MSA), Giza, Egypt.
  • Google Scholar
Reham Wasfi
  • Reham Wasfi
  • Microbiology and Immunology Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza, Egypt.
  • Google Scholar
Noha Swilam
  • Noha Swilam
  • Pharmacognosy Department, Faculty of Pharmacy, British University in Egypt (BUE), Cairo, Egypt.
  • Google Scholar
Ibrahim Ezz Sallam
  • Ibrahim Ezz Sallam
  • Pharmacognosy Department, October University for Modern Sciences and Arts (MSA), Giza, Egypt.
  • Google Scholar


  •  Received: 29 May 2016
  •  Accepted: 05 July 2016
  •  Published: 10 July 2016

Abstract

Anti-biofilm represents an urge to face drug resistance. Nymphaea alba L. flowers and rhizomes have been traditionally used in Ayurvedic medicine for dyspepsia, enteritis, diarrhea and as an antiseptic. This study was designed to identify the main constituents of Nymphaea alba L. rhizomes and their anti-biofilm activity. 70% aqueous ethanolic extract (AEE) of N. alba rhizomes was analyzed by liquid chromatography, high resolution, mass spectrometry (LC-HRMS) for its phytoconstituents in the positive and negative modes in addition to column chromatographic separation. Sixty-four phenolic compounds were identified for the first time in N. alba rhizomes. Hydrolysable tannins represent the majority with identification of galloyl hexoside derivative, hexahydroxydiphenic (HHDP) derivatives, glycosylated phenolic acids and glycosylated flavonoids. Five phenolics have been isolated and identified as gallic acid and its methyl and ethyl ester in addition to ellagic acid and pentagalloyl glucose. Minimum inhibitory concentrations (MIC) and anti-biofilm activity for the extract and the major isolated compounds were determined. Radical scavenging activity using 2.2Di (4-tert-octylphenyl)-1-picryl-hydrazyl (DPPH) assay as well as cytotoxic activity using 3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay have also been evaluated. MIC of N. alba rhizomes against Staphylococcus aureus was 0.25 mg/mL compared with 0.1 mg/mL for methyl gallate. The best reduction in biofilm formation (84.9%) as well as the best radical scavenging (IC50 3 µg/mL) and cytotoxic (IC50 9.61 ± 0.3 µg/mL) activities were observed with methyl gallate. This is the first study for in-depth characterization of phenolic compounds in N. alba rhizomes revealing it as a valuable source of phenolic compounds and promising anti-biofilm forming agent of natural origin.

 

Key words: Hydrolysable tannins, Nymphaeaceae, LC-HRMS.