Journal of Medicinal Plants Research
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Article Number - DE4F1DB55875


Vol.12(3), pp. 42-49 , January 2018
https://doi.org/10.5897/JMPR2017.6528
ISSN: 1996-0875


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Full Length Research Paper

Evaluation of essential oils from 22 Guatemalan medicinal plants for in vitro activity against cancer and established cell lines



Andrew Byron Miller
  • Andrew Byron Miller
  • College of Life Sciences, Brigham Young University (BYU), Provo, UT USA.
  • Google Scholar
Rex Gordon Cates
  • Rex Gordon Cates
  • College of Life Sciences, Brigham Young University (BYU), Provo, UT USA.
  • Google Scholar
Kim O’Neill
  • Kim O’Neill
  • College of Life Sciences, Brigham Young University (BYU), Provo, UT USA.
  • Google Scholar
Juan Alfonso Fuentes Soria
  • Juan Alfonso Fuentes Soria
  • Secretaria General del Consejo, Superior Universitario de Centroamerica (CSUCA), Ave. Las Americas 1-03, Zona 14, Interior Club Los Arcos, Guatemala City, Guatermala.
  • Google Scholar
Luis Vicente Espinoza
  • Luis Vicente Espinoza
  • Benson Agriculture and Food Institute, Welfare Services, Salt Lake City, Utah (UT), USA.
  • Google Scholar
Ballantines Fabrizio Alegre
  • Ballantines Fabrizio Alegre
  • College of Life Sciences, Brigham Young University (BYU), Provo, UT USA.
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Jose Vicente Martinez
  • Jose Vicente Martinez
  • Facultad de Agronomía, Edificio T-8, Ciudad Universitaria, Zona No.12, Guatemala City, Guatemala.
  • Google Scholar
Dany Raul Arbizu
  • Dany Raul Arbizu
  • Benson Institute Guatemala, Chiquimula, Guatemala.
  • Google Scholar







 Received: 24 October 2017  Accepted: 21 December 2017  Published: 17 January 2018

Copyright © 2018 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Plant species which produce essential oils are important in the healthcare of rural Guatemalans. Steam distilled essential oils from 22 medicinal plant species were analyzed for activity against tongue, skin, and stomach cancer cell lines using a neutral red assay, Vero C1008 cells to assess cytotoxicity, and [3H]-thymidine incorporation assay to assess inhibition of cancer cell proliferation. IC50, CC50, and therapeutic indices were determined. IC50 values indicated that all oils showed inhibitory activity against one or more cancer cell lines.  Highly inhibitory IC50 values (0.10 μL/mL or less) indicated that Citrus aurantiifolia (Christm.) Swingle (Rutaceae) oil was significantly inhibitory to all three cancer cell lines, Origanum vulgare L. (Lamiaceae) and Teloxys ambrosioides (L.) W. A. Weber (Chenopodiaceae) oils were highly inhibitory to two cell lines, and Lippia graveolens Kunth (Verbenaceae) oil was highly inhibitory to one cell line.  TI values equal to or greater than one showed significantly higher cytotoxicity to cancer cells compared to the Vero cell line for Ruta chalepensis L. (Rutaceae), Citrus limetta Risso (Rutaceae), C. aurantium L. (Rutaceae), Rosmarinus officinalis L. (Lamiaceae), and O. vulgare.  Essential oils from L. graveolens, O. vulgare, and T. ambrosioides yielded high percentages (>96%) of decreased cell proliferation at low oil concentration (0.05 μL/mL). Results indicate that essential oils were more toxic to cancer cells than to cells from an established cell line, and such oils can be highly suppressive to DNA synthesis and cancer cell growth. 

Key words:  Essential oils, medicinal plants, IC50, anticancer activity, Guatemala.

 

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APA Miller, A. B., Cates., R. G., O’Neill, K., Soria, J. A. F., Espinoza, L. V., Alegre, B. F., Martinez, J. V., & Arbizu, D. R. (2018). Evaluation of essential oils from 22 Guatemalan medicinal plants for in vitro activity against cancer and established cell lines. Journal of Medicinal Plants Research, 12(3), 42-49.
Chicago Andrew Byron Miller, Rex Gordon Cates, Kim O’Neill, Juan Alfonso Fuentes Soria, Luis Vicente Espinoza, Ballantines Fabrizio Alegre, Jose Vicente Martinez and  Dany Raul Arbizu  . "Evaluation of essential oils from 22 Guatemalan medicinal plants for in vitro activity against cancer and established cell lines." Journal of Medicinal Plants Research 12, no. 3 (2018): 42-49.
MLA , et al. "Evaluation of essential oils from 22 Guatemalan medicinal plants for in vitro activity against cancer and established cell lines." Journal of Medicinal Plants Research 12.3 (2018): 42-49.
   
DOI https://doi.org/10.5897/JMPR2017.6528
URL http://academicjournals.org/journal/JMPR/article-abstract/DE4F1DB55875

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