Journal of
Physiology and Pathophysiology

  • Abbreviation: J. Physiol. Pathophysiol.
  • Language: English
  • ISSN: 2141-260X
  • DOI: 10.5897/JPAP
  • Start Year: 2010
  • Published Articles: 41

Full Length Research Paper

Attenuation of allergic airways inflammation by an extract of Hymenocardia acida

Fatou Bintou Sar*
Mamadou Sarr*
Mama S.Y. Diallo
  • Mama S.Y. Diallo
  • Laboratoire d?Histologie et d?Embryologie, FMPO, UCAD, Dakar, Senegal.
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Saliou Ngom
  • Saliou Ngom
  • Laboratoire d?innovation thérapeutique, UMR 7200, Faculté de Pharmacie, Illkirch, Université de Strasbourg, France
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Lamine Gueye
  • Lamine Gueye
  • Laboratoire de Physiologie et Explorations fonctionnelles, FMPO, UCAD, Dakar, Senegal. Unité Mixte Internationale de Recherches (UMI 3189) ?Environnement, Santé, Sociétés? CNRS-UCAD-CNRST- USTTB-UGB, Dakar, Senegal.
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Abdoulaye Samb
  • Abdoulaye Samb
  • Laboratoire de Physiologie et Explorations fonctionnelles, FMPO, UCAD, Dakar, Senegal. Unité Mixte Internationale de Recherches (UMI 3189) ?Environnement, Santé, Sociétés? CNRS-UCAD-CNRST- USTTB-UGB, Dakar, Senegal.
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Ramaroson Andriantsitohaina
  • Ramaroson Andriantsitohaina
  • INSERM U1063, Stress oxydant et pathologies métaboliques, Angers, France
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Annelise Lobstein
  • Annelise Lobstein
  • Laboratoire d?innovation thérapeutique, UMR 7200, Faculté de Pharmacie, Illkirch, Université de Strasbourg, France.
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  •  Received: 21 June 2014
  •  Accepted: 23 October 2014
  •  Published: 30 November 2014

Abstract

Tracheal hyperresponsiveness, airway mucus production and bronchoalveolar inflammation are the major components of asthma. Here, we aim to investigate the role in the control of asthma of a bioactive plant extracted from Hymenocardia acida in a physiological and pathophysiological model. The effect of H. acida crude extract (HACE) on total cellular components of bronchoalveolar (BAL) fluids was performed on ovalbumin (OVA) and lipopolysaccaride (LPS)-challenged Swiss mice for induction of allergic asthma and airways inflammation, respectively. Mice were pretreated with 0.9% sodium chloride (NaCl), HACE (oral doses at 100 mg/kg/body weight) for a week and then by intranasal instillation with OVA (0.5 mg/ml) + aluminium hydroxyde (20 mg/ml), during three days after intraperitoneally sensitization or with LPS (0.4 mg/ml) for a day (OVA or LPS + HACE). The BAL cells were collected in a mixed solution (0.9% NaCl and 2.6 mm Ethylenediaminetetraacetic acid EDTA) one day after the last challenge and total cells were numbered in a Neubauer chamber. The HACE: (i) significantly inhibited the airways inflammation induced by a single intranasal instillation of LPS or allergic asthma on mice challenged with 3 consecutive days intranasal instillation of OVA in comparison to control mice only instilled with 0.9% sterile. NaCl : (ii) significantly impaired the increased levels of total cells in OVA and LPS-treated mice, without changing the basal cellularity after NaCl or HACE treatment; (iii) and significantly inhibitshydroxyl radicalsandsuperoxideanions production. Taken together, these results suggest that HACE exposure induces a marked reduction of cellular component in the BAL fluid, which is only partially lymphocytes dependent.

 

Key words: Asthma, prevention, hymenocardia acida, mice.